Raising the prices of crude petroleum is affected to good performance for the asphalt paving and has encouraged to modify or replace asphalt bitumen. Increasing numbers of commercial vehicles and increase axle load that this trend will continue yearly. Design, construction and maintenance facilitate asphalt roads to provide the high standards of safety and comfortable but certain areas of a road are higher stressed than others. For that reason road paving industry is interested in utilizing alternative and sustainable binder materials for modified asphalt to improve the production, placement and performance of asphalt mixtures. However, sources of materials should be considered economically and environtmentally viable after applied in pavement. According to previous research, that has been developed that non crude petroleum binder derived from the production of bio-oil through fast pyrolysis of biomass. The main source of bio-oil can be contributed from biomass industry or renewable organic industry such as timber waste, oil palm waste, rice husk; coconut trunk fibers, municipal waste and sugar cane waste. Alternatively, the bio-oil can be as substitute material as modification of asphalt. This paper presents a review on the source, characteristic of bio-oil and the effects of bio-oil on the properties of asphalt bitumen.