Effect of arginine on IL-6, IL-17 and TGF-β levels in high-fat diet-induced hypercholesterolemia rat

Abstract

Objective: The aim with the present study was to determine that arginine affect to the level of IL-6, IL-17 and TGF-β in high-fat diet-induced hypercholesterolemia in rats. Material and Methods: Male Wistar rats in eight weeks old (n=35) were divided into five groups, one group as a control (C) were given standard r fed and four groups were given high-fat diet (Hfd) with fats from beef tallow. One group of high-fat diet rats recieved no arginine, three groups were recieved arginine in doses of 100, 200 and 400 mg/kg/day each for three weeks. After two weeks of treatment, blood cholesterol were measured, while serum level of IL-6, IL-17 and TGF-β were measured at the final five weeks of this protocol by ELISA methods. Results: There were no significantly different between groups, which received different doses of arginine (p>0,05). IL-17 of high-fat diet received arginine of doses 100 and 200 mg/kg/day were significantly higher compared to high-fat diet received no arginine and control group. Interestingly, IL-17 in high-fat diet group received arginine of doses 400 mg/kg/day were lower compared to groups received lower doses of arginine. TGF-β levels in the high-fat diet group's recieved arginine were lower compared to control group. Conclusions: The effect of arginine on cytokines plays the role on vascular inflammation due to high-fat diet. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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