Effect of Aromatic Nitroxides on Hemolysis of Human Erythrocytes Entrapped With Isolated Hemoglobin Chains

Gabbianelli, Rosita; Falcioni, Giancarlo; Santroni, Anna Maria; Caulini, G; Greci, Lucedio; Damiani, Elisabetta

doi:10.1016/s0891-5849(96)00611-9

Cited by 20 publications

(8 citation statements)

References 32 publications

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“…The possible involvement of ␤97 His in the shift toward the R-form, is in agreement with the hypothesis of Perutz and Brunori, who suggested an involvement of this His in T-form stabilization. 24 The results of the present work could explain previous data 9 which demonstrated a TBTC induced precipitation of HbIV, which is usually correlated with an increased oxidation rate of Fe(II), 25 probably affected by an increased affinity for oxygen. In the same wavelength range the organotin does not induce any change on HbI spectrum, in line with previous work 9 indicating that TBTC protects the HbI, by stabilizing the Fe(II) against oxidation.…”

Section: Discussionsupporting

confidence: 84%

Steady-state fluorescence and circular dichroism of trout hemoglobins I and IV interacting with tributyltin

et al. 1999

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The effect of tributyltin chloride (TBTC) on rainbow trout (Salmo irideus) hemoglo-bin I (HbI) and hemoglobin IV (HbIV) was characterized by the steady-state fluorescence of intrinsic and extrinsic fluorescent probes. The fluorescence emission spectrum (ex 280 nm) is greatly increased in intensity by the presence of the organotin in both proteins. Circular dichroism spectra in the same samples show a small decrease in 222 , a measure correlated with the percentage of the-helical content. Morever, important changes in near-UV, Soret, and visible regions of CD were induced by TBTC. The correlation of data obtained with trout hemoglo-bins (HbI and HbIV) with similar measurements on globins suggests that the presence of heme is necessary for the interaction of the organotin compound with the proteins. Proteins 1999;34:443-452. 1999 Wiley-Liss, Inc.

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Section: Discussionsupporting

confidence: 84%

Steady-state fluorescence and circular dichroism of trout hemoglobins I and IV interacting with tributyltin

et al. 1999

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“…When the light signal obtained from the excited state of the chemical reaction product is low, it is necessary to amplify it using a probe that does not interfere with the reaction (Murphy and Sies, 1990). Usually, luminol and lucigenin are employed to detect hydrogen peroxide and superoxide anion, respectively (Olek et al, 2005;Gabbianelli et al, 1997). The latter ROS is an important phagocyte respiratory burst product (Babior, 1984(Babior, , 1987.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of ethyl pyruvate on msP rat leukocytes damaged by alcohol intake

Fedeli¹,

Falcioni²,

Olek³

et al. 2007

J of Applied Toxicology

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Alcohol consumption for long periods negatively influences physiological functions of many cells, and leads to organ damage. Reactive oxygen and nitrogen species produced by ethanol metabolism cause adverse effects that might be alleviated by simultaneous treatment with various antioxidants. Here, the ability of ethyl pyruvate (EP) to reduce ethanol-induced oxidative stress was evaluated. Chemiluminescence studies show that EP has a higher capacity than pyruvate to scavenge hydrogen peroxide and superoxide anions. In order to evaluate whether EP can exert a protective effect against ethanol, rats were offered 10% ethanol in drinking burettes, containing or not different concentrations of EP (0.3%, 1% and 3%). The comet assay was employed to quantify the alcohol-induced DNA damage in rat lymphocytes. This test is a promising tool for the estimation of DNA damage at the single cell level. A significant protective effect of EP was observed in rat groups treated with this antioxidant, compared with those drinking only ethanol. Since EP has been shown to decrease the expression of numerous pro-inflammatory mediators, the monocyte respiratory burst was evaluated. The activation of monocyte NADPH oxidase by phorbol esters (PMA) showed that superoxide anion production was higher in the ethanol group than in the control group. The presence of EP considerably reduced superoxide anion production. In conclusion, hypotheses on possible mechanisms of action of EP on rat white blood cells are proposed.

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“…The lipid peroxidation can be evaluated in plasma membrane by the measurement of conjugated dienes formation (Falcioni et al, 1998;Gabbianelli et al, 1997). In fact, in polyunsaturated fatty acids, the oxidative process starts with the formation of a carbon radical which stabilizes to form a conjugated dienes.…”

Section: Effect On Lipid Peroxidationmentioning

confidence: 99%

Lymphocyte DNA damage in rats exposed to pyrethroids: effect of supplementation with Vitamins E and C

et al. 2004

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Effect of Aromatic Nitroxides on Hemolysis of Human Erythrocytes Entrapped With Isolated Hemoglobin Chains

Cited by 20 publications

References 32 publications

Steady-state fluorescence and circular dichroism of trout hemoglobins I and IV interacting with tributyltin

Steady-state fluorescence and circular dichroism of trout hemoglobins I and IV interacting with tributyltin

Protective effect of ethyl pyruvate on msP rat leukocytes damaged by alcohol intake

Lymphocyte DNA damage in rats exposed to pyrethroids: effect of supplementation with Vitamins E and C

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