Effect of biotin limitation on the conversion of xylose to ethanol and xylitol by Pachysolen tannophilus and Candida guilliermondii

Lee, Hung; Atkin, Audrey L.; Barbosa, M.F.S.; Dorscheid, Delbert R.; Schneider, Henry

doi:10.1016/0141-0229(88)90002-6

Cited by 36 publications

(5 citation statements)

References 16 publications

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“…As the hemicellulosic fraction contains polymers of other sugars, the chemical process includes expensive separation and purification steps to remove these byproducts from xylose or xylitol (Hyvo ¨nen and Koivistoinen, 1983). It can also be produced by microbiological methods with xylose utilizing yeasts (Du Preez, 1994;Du Preez et al, 1989a;Gong et al, 1981;Gong et al, 1983;Horitsu et al, 1992;Lee et al, 1988;Meyrial et al, 1991;Nishio et al, 1989;Smiley and Bolen, 1982;Vongsuvanlert and Tani, 1989;Yahashi et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Increase of xylitol production rate by controlling redox potential inCandida parapsilosis

Oh¹,

Kim²,

Kim³

1998

Biotechnol. Bioeng.

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The effect of redox potential on xylitol production by Candida parapsilosis was investigated. The redox potential was found to be useful for monitoring the dissolved oxygen (DO) level in culture media, especially when the DO level was low. An increase in the agitation speed in a 5 L fermentor resulted in an increased culture redox potential as well as enhanced cell growth. Production of xylitol was maximized at a redox potential of 100 mV. As the initial cell concentration increased from 8 g/L to 30 g/L, the volumetric productivity of xylitol increased from 1.38 g/L · h to 4.62 g/L · h. A two‐stage xylitol production strategy was devised, with stage 1 involving rapid production of cells under well‐aerated conditions, and stage 2 involving cultivation with reduced aeration such that the culture redox potential was 100 mV. Using this technique, a final xylitol concentration of 180 g/L was obtained from a culture medium totally containing 254.5 g/L xylose in a 3,000 L pilot scale fermentor after 77 h fermentation. The volumetric productivity of xylitol during the fermentation was 2.34 g/L · h. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:440‐444, 1998.

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Section: Introductionmentioning

confidence: 99%

Increase of xylitol production rate by controlling redox potential inCandida parapsilosis

Oh¹,

Kim²,

Kim³

1998

Biotechnol. Bioeng.

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“…Fermentation of the water-soluble fraction (hydrosylate) obtained after steam pretreatment of hardwood and softwood mixture To characterize Rhodotorula mucilaginosa strain PTD3's fermentability of lignocellulosic hydrolysates, direct comparison with the well-known xylitol and ethanol producer Candida guilliermondii [11,18,32] was carried out.…”

Section: Mixed Synthetic Sugar Fermentationmentioning

confidence: 99%

Novel endophytic yeast Rhodotorula mucilaginosa strain PTD3 I: production of xylitol and ethanol

Bura

Vajzovic

Doty

2012

Journal of Industrial Microbiology and Biotechnology

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An endophytic yeast, Rhodotorula mucilaginosa strain PTD3, that was isolated from stems of hybrid poplar was found to be capable of production of xylitol from xylose, of ethanol from glucose, galactose, and mannose, and of arabitol from arabinose. The utilization of 30 g/L of each of the five sugars during fermentation by PTD3 was studied in liquid batch cultures. Glucose-acclimated PTD3 produced enhanced yields of xylitol (67% of theoretical yield) from xylose and of ethanol (84, 86, and 94% of theoretical yield, respectively) from glucose, galactose, and mannose. Additionally, this yeast was capable of metabolizing high concentrations of mixed sugars (150 g/L), with high yields of xylitol (61% of theoretical yield) and ethanol (83% of theoretical yield). A 1:1 glucose:xylose ratio with 30 g/L of each during double sugar fermentation did not affect PTD3's ability to produce high yields of xylitol (65% of theoretical yield) and ethanol (92% of theoretical yield). Surprisingly, the highest yields of xylitol (76% of theoretical yield) and ethanol (100% of theoretical yield) were observed during fermentation of sugars present in the lignocellulosic hydrolysate obtained after steam pretreatment of a mixture of hybrid poplar and Douglas fir. PTD3 demonstrated an exceptional ability to ferment the hydrolysate, overcome hexose repression of xylose utilization with a short lag period of 10 h, and tolerate sugar degradation products. In direct comparison, PTD3 had higher xylitol yields from the mixed sugar hydrolysate compared with the widely studied and used xylitol producer Candida guilliermondii.

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“…At various times, 1 ml samples were removed and processed for metabolite analyses [1,10]. To study the effect of a hexose on D-xylose utilization, both D-xylose and the hexose were added to the medium.…”

Section: Sugar Utilization In Liquid Culturesmentioning

confidence: 99%

Differential fructose effect in Pachysolen tannophilus and Pichia stipitis

Bicho

Cunningham

Lee

1989

FEMS Microbiology Letters

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The yeasts Pachysolen tannophilus and Pichia stipitis differed in their ability to utilize d‐xylose in the presence of d‐fructose. When P. tannophilus was grown aerobically in a fructose‐xylose mixture, the ketohexose was utilized preferentially over the pentose. However, in P. stipitis cultures, the converse was observed. The effect was associated with the ability of d‐fructose to repress the induction of xylose reductase and xylitol dehydrogenase activities in P. tannophilus but not in P. stipitis. Both yeasts grew on d‐fructose and fermented it to ethanol when it was supplied as the sole carbon source. The results suggest that there may exist some fundamental difference in the regulation of d‐fructose metabolism between P. tannophilus and P. stipitis.

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Effect of biotin limitation on the conversion of xylose to ethanol and xylitol by Pachysolen tannophilus and Candida guilliermondii

Cited by 36 publications

References 16 publications

Increase of xylitol production rate by controlling redox potential inCandida parapsilosis

Increase of xylitol production rate by controlling redox potential inCandida parapsilosis

Novel endophytic yeast Rhodotorula mucilaginosa strain PTD3 I: production of xylitol and ethanol

Differential fructose effect in Pachysolen tannophilus and Pichia stipitis

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