Effect of bone marrow mesenchymal stem cell transplantation combined with lugua polypeptide injection on osteoarthritis in rabbit knee joint

Huang, Huajun; Zhang, Ping; Xiang, Chunling; Zeng, Canjun; Du, Qing-jun; Huang, Wenhua

doi:10.1080/03008207.2021.1962314

Cited by 6 publications

(8 citation statements)

References 14 publications

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“…Ten studies investigated indirect disease-modifying effects through the measurement of serum or synovial fluid biomarkers related to cartilage metabolism or inflammation. Half of these studies reported positive results compared to OA controls, documenting an increase of the serum levels of IL-10 and a decrease of the synovial fluid levels of IL-1β, IL-6, MMP-1, MMP-13, nitric oxide (NO), prostaglandin E2 (PGE2), TGF-β, and TNF-α [ 28 , 32 , 39 , 44 , 45 ].…”

Section: Resultsmentioning

confidence: 99%

“…Twenty studies compared the disease-modifying effects of bone marrow-derived products differing in dose, preparation, injective protocol, or additional procedures/pre-treatments. In detail, 8 studies evaluated the benefits offered by different additional procedures, reporting better results compared to BMSCs alone in case of preconditioning with Kartogenin or Vitamin E, supplementation with curcumin, transfection with transforming growth factor receptor I (ALK5) plasmid or adenoviral vectors expressing vIL-10 (AdIL-10), and pre-treatment with the autophagy inhibitor 3-Methyladenine or stimulation with low-intensity pulsed ultrasound [ 11 , 33 , 37 , 39 , 49 , 55 , 56 ]. No overall advantages were observed in case of priming with TNF-α and interferon-γ (IFN-γ) [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…Finally, one study demonstrated that the combined use of BMSCs and Lugua polypeptide injections had lower synovial fluid level of inflammatory biomarkers compared to Lugua polypeptide injection alone [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

“…Immunohistochemical evaluations revealed an increased expression of aggrecan and collagen type-II alpha and a decreased expression of collagen type-I alpha [11,33,35,37]. Finally, bone marrow-derived products induced in chondrocytes a higher mRNA/gene expression of type-II collagen, aggrecan, B-cell lymphoma 2 (BCL-2), cyclin D1, proliferating cell nuclear antigen (PCNA), transforming growth factor beta (TGF-β), and tissue inhibitor of metalloproteinases 1 (TIMP-1), and a lower mRNA/gene expression of type-I collagen, Adamts-4, BCL-2-like protein 4 (Bax), Caspase-3, cyclooxygenase 2 (COX-2), interleukin-1 beta (IL-1β), matrix metalloproteinase 1 (MMP-1), MMP-4, MMP-13, nuclear factor-kappa B (NF-Κb) p65, protein P21, and vascular endothelial growth factor VEGF [22,24,33,[38][39][40][41][42].…”

Section: Disease-modifying Efects At Cartilage Levelmentioning

confidence: 99%

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Cell-based therapies have disease-modifying effects on osteoarthritis in animal models. A systematic review by the ESSKA Orthobiologic Initiative. Part 2: bone marrow-derived cell-based injectable therapies

Boffa

Orfei²,

Sourugeon

et al. 2023

Knee Surg Sports Traumatol Arthrosc

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Purpose Aim of this systematic review was to determine if bone marrow-derived cell-based injectable therapies induce disease-modifying effects in joints affected by osteoarthritis (OA) in animal models. Methods A systematic review was performed on three electronic databases (PubMed, Web of Science, Embase) according to PRISMA guidelines. A synthesis of the results was performed investigating disease-modifying effects in preclinical animal studies comparing injectable bone marrow-derived products with OA controls or other products, different formulations or injection intervals, and the combination with other products. The risk of bias was assessed according to the SYRCLE’s tool. Results Fifty-three studies were included (1819 animals) with an increasing publication trend over time. Expanded cells were used in 48 studies, point-of-care products in 3 studies, and both approaches were investigated in 2 studies. Among the 47 studies presenting results on the disease-modifying effects, 40 studies (85%) reported better results with bone marrow-derived products compared to OA controls, with positive findings evident in 14 out of 20 studies (70%) in macroscopic assessment, in 30 out of 41 studies (73%) in histological assessment, and in 10 out of 13 studies (77%) in immunohistochemical evaluations. Clinical evaluations showed positive results in 7 studies out of 9 (78%), positive imaging results in 11 studies out of 17 (65%), and positive biomarker results in 5 studies out of 10 (50%). While 36 out of 46 studies (78%) reported positive results at the cartilage level, only 3 out of 10 studies (30%) could detect positive changes at the synovial level. The risk of bias was low in 42% of items, unclear in 50%, and high in 8%. Conclusion This systematic review of preclinical studies demonstrated that intra-articular injections of bone marrow-derived products can induce disease-modifying effects in the treatment of OA, slowing down the progression of cartilage damage with benefits at macroscopic, histological, and immunohistochemical levels. Positive results have been also observed in terms of clinical and imaging findings, as well as in the modulation of inflammatory and cartilage biomarkers, while poor effects have been described on the synovial membrane. These findings are important to understand the potential of bone marrow-derived products and to guide further research to optimise their use in the clinical practice. Level of evidence II.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Disease-modifying Efects At Cartilage Levelmentioning

confidence: 99%

See 2 more Smart Citations

Cell-based therapies have disease-modifying effects on osteoarthritis in animal models. A systematic review by the ESSKA Orthobiologic Initiative. Part 2: bone marrow-derived cell-based injectable therapies

Boffa

Orfei²,

Sourugeon

et al. 2023

Knee Surg Sports Traumatol Arthrosc

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“…In contrast, KOA rabbits treated in hUCB-MNC group exhibited smoother articular surfaces, more aligned chondrocytes, and relatively intact cartilage layers. One study reported that bone marrow mesenchymal stem cells reduced TNF-α, IL-1β in the joint uid of rabbit KOA models, thereby reducing in ammation [31] .…”

Section: Discussionmentioning

confidence: 99%

The effect of umbilical cord blood mononucleated cells on knee osteoarthritis in rabbits

傅,

张,

杨

et al. 2024

Preprint

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Objective To investigate the effect of umbilical cord blood-mononuclear cells (UCB-MNC) on the treatment of knee osteoarthritis (KOA) in rabbits and its possible mechanism. Methods The rabbit knee osteoarthritis model was prepared by anterior cruciate ligament (ACLT) method. Fifty New Zealand white rabbits were randomly divided into the Control group, Model group, sodium hyaluronate (SH) group, platelet rich plasma (PRP) group, and UCB-MNC group. Knee cavity injection was performed once a week for 5 consecutive weeks. The gross view of the knee joint, the cartilage morphology of the knee joint, and the structural changes of the knee joint in imaging were observed by behavior, gross shape of the knee joint, HE staining, and CT. The levels of TNF-α and IL-1β in rabbit knee fluid were detected by ELISA. Western blot and QRT-PCR were used to detect the expression of MMP-13 and COL-II protein and mRNA in knee cartilage. Results From the behavioral Lequesne MG scores, morphological HE staining Mankin's scores, the scores of model group were significantly higher compared with that of control group. The SH, PRP, and UCB-MNC group had sequentially lower scores compared to theModel group. From CT, KOA features were more pronounced in the model group compared to other groups, and there was a slight improvement in imaging knee osteoarthritis in the CB-MNC group compared to the SH and PRP groups. Compared with the control group, the Model group had increased levels of TNF-α,

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Advances in understanding effects of miRNAs on apoptosis, autophagy, and pyroptosis in knee osteoarthritis

An,

Sun,

Liu

et al. 2023

Mol Genet Genomics

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Effect of bone marrow mesenchymal stem cell transplantation combined with lugua polypeptide injection on osteoarthritis in rabbit knee joint

Cited by 6 publications

References 14 publications

Cell-based therapies have disease-modifying effects on osteoarthritis in animal models. A systematic review by the ESSKA Orthobiologic Initiative. Part 2: bone marrow-derived cell-based injectable therapies

Cell-based therapies have disease-modifying effects on osteoarthritis in animal models. A systematic review by the ESSKA Orthobiologic Initiative. Part 2: bone marrow-derived cell-based injectable therapies

The effect of umbilical cord blood mononucleated cells on knee osteoarthritis in rabbits

Advances in understanding effects of miRNAs on apoptosis, autophagy, and pyroptosis in knee osteoarthritis

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