Effect of bovine colostrum on the growth inhibition and differentiation of human leukemic U937 cells

Ma, Chia-Yu; Kung, Hua-Ting; Chen, Jiannan; Chang, Ching-Yu; Chang, Huang-Min; Wu, James Swi‐Bea

doi:10.1002/jsfa.3539

Cited by 4 publications

(15 citation statements)

References 26 publications

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“…Growth inhibition of the U937 cells treated with PIS‐, ALS‐ and FLS‐MNC‐CM (500 µg/mL) for 3 days was 59.36, 55.00 and 57.48%, respectively (data not shown). The results were similar to those reported by Ma et al . (2009) who found that the inhibition of U937 growth using MNC‐CM prepared with colostrums collected on the second postpartum day at 100–800 µg/mL was 43.3 to 55.9%.…”

Section: Resultssupporting

confidence: 93%

“…2005); cells prepared with the water extract of rice ( Oryza sativa L.) at 500 µg/mL was 35% (Liao et al . 2006); bovine skimmed colostrums collected on the second day postpartum at 800 µg/mL was 50% (Ma et al . 2009); and semi‐purified polysaccharides of Antrodia camphorate at 100 µg/mL was 55% (Liu et al .…”

Section: Discussionmentioning

confidence: 99%

“…2008); having an impact on bacterial physiological activities (Swiatecka et al . 2010) and immunological regulatory activities (Ma et al . 2009).…”

Section: Introductionmentioning

confidence: 99%

“…(1993) showed that bovine colostrums enhanced immunity against crytosporidiume, staphenterotoxins and pertussis on murine. Ma et al . (2009), through the use of an indirect model, found that bovine colostrums were capable of enhancing immunity against human leukemic U937 cell death.…”

Section: Introductionmentioning

confidence: 99%

“…Some protein hydrolysates have been found to exhibit functional uses. These include acting as antioxidants (Chiang and Chang 2005;Je et al 2005Je et al , 2009Chang et al 2007;Hus 2010); performing angiotensin I-converting enzyme inhibitory activities (Pan et al 2005;Wu et al 2008); having an impact on bacterial physiological activities (Swiatecka et al 2010) and immunological regulatory activities (Ma et al 2009). Watzl et al (1993) showed that bovine colostrums enhanced immunity against crytosporidiume, staphenterotoxins and pertussis on murine.…”

Section: Introductionmentioning

confidence: 99%

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Growth Inhibition and Differentiating Effects of Protein Hydrolysates From Bovine Colostrums on Human Leukemic U937 Cells

Chen¹,

Chiang²,

Wang³

et al. 2011

Journal of Food Biochemistry

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This research investigates the effects of bovine colostrums and their protein hydrolysates on human mononuclear cell (MNC) growth, on the secretion of cytokines (interleukin‐1β, interferon‐γ and tumor necrosis factor‐α) as well as nitric oxide, and on the growth inhibition of human leukemic U937 cells. The results indicate that the colostrum hydrolysates obtained by porcine small‐intestinal enzymes (PIS) exhibit more significant inhibitory effect on U937 cell growth than do the caseins (PIC) and the whey (PIW) hydrolysates (PIS 57.45%, PIC 42.58% and PIW 48.47% at 1,000 µg/mL). The greatest growth index of MNC, up to 1.43, was achieved by a treatment of PIS at 300 µg/mL for 3 days. The cytokines (IL‐1β, TNF‐α and IFN‐γ) secretion of MNC by a treatment of PIS for 3 days (at 800 µg/mL) was 4,443.35, 325.04 and 4,649.67 pg/mL, respectively. The results suggest that bovine colostrum protein hydrolysates may be utilized in functional food. PRACTICAL APPLICATIONS Large quantities of protein‐rich bovine colostrums are used for feeding calves or discarded as waste because this glutinous, yellowish fluid is also bitter tasting and coagulates easily upon heating. Such waste is a good source for the extraction of bioactive molecules such as proteins, growth factors, antimicrobial compounds and immunological components. The results of this research shed light on the effects of bovine colostrums and their protein hydrolysates on the immunomodulatory activities and the growth inhibition of U937 cells. They may help in the discovery of new applications while reducing the considerable problem caused by waste disposal. The use of food protein hydrolysates is widely accepted in the field of cosmetics and healthcare products. Bovine colostrum protein hydrolysate products can be useful to enhance human health. Our results point to the possibility that colostrums may be processed into an antileukemic drug or may be used as an immunomodulatory activity ingredient in health foods.

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Section: Resultssupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

“…2008); having an impact on bacterial physiological activities (Swiatecka et al . 2010) and immunological regulatory activities (Ma et al . 2009).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Growth Inhibition and Differentiating Effects of Protein Hydrolysates From Bovine Colostrums on Human Leukemic U937 Cells

Chen¹,

Chiang²,

Wang³

et al. 2011

Journal of Food Biochemistry

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Effects of vinegar–egg on growth inhibition, differentiation human leukemic U937 cells and its immunomodulatory activity

Wang

Chang

Chen

2018

Journal of Food and Drug Analysis

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Vinegar and eggs have rich nutrients. In this study, the mixed form of both derived products, vinegar-egg solution and its products (vinegar-egg concentrate and vinegar-egg condensate) were chosen for an assessment of their biological activity. To further our understanding regarding the anticancer and immunomodulatory effects of vinegar-egg, we investigated its effects on the proliferation and differentiation of U937 cells. Vinegar-egg was treated using spray drying, freeze drying and vacuum concentration and used to stimulate human mononuclear cells. The conditioned media obtained from these cultures by filtration were used to treat U937 cells. Three conditioned media inhibited U937 cell growth by 22.1-67.25% more effectively than PHA-treated control (22.53%). CD11b and CD14 expression on the treated U937 cells were 29.1-45.4% and 31.6-47.2%, respectively. High levels of cytokines IL-1β, IFN-γ and TNF-α were detected in the three conditioned media. Vinegar-egg stimulates human mononuclear cells to secrete cytokines, which inhibit the growth of U937 cells and induce their differentiation.

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In Vitro Antitumor and Immunomodulatory Effects of the Protein PCP-3A from Mushroom Pleurotus citrinopileatus

Chen

Ma²,

Tsai

et al. 2010

J. Agric. Food Chem.

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A nonlectin glycoprotein (PCP-3A) newly isolated from the fruit body of edible golden oyster mushroom Pleurotus citrinopileatus has been shown to be growth inhibitory against human myeloid leukemic U937 cells in a previous report. There is a well-recognized relation between antitumor activity and immunomodulation. The immunomodulatory activity of PCP-3A was therefore assessed in the present study. Human mononuclear cells (MNC) and the CD4(+) T lymphocytes isolated from them were stimulated separately with PCP-3A for various durations and then filtered to obtain the conditioned media (CM). The conditioned medium from MNC (MNC-CM) was proved effective in inhibiting the growth of U937 cells. Increased secretion of cytokines TNF-α, IL-2, and IFN-γ from the stimulated MNC and CD4(+) T cells was found in CM. The antibody neutralization test of MNC-CM revealed that the growth inhibition on leukemic U937 cells was related to the elevation in cytokine concentration. We propose that PCP-3A stimulated human MNC to secrete cytokines TNF-α, IL-2, and IFN-γ, which subsequently inhibit the growth of U937 cells, and that PCP-3A may be a possible material for developing into an antileukemia ingredient in health food.

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Effect of bovine colostrum on the growth inhibition and differentiation of human leukemic U937 cells

Cited by 4 publications

References 26 publications

Growth Inhibition and Differentiating Effects of Protein Hydrolysates From Bovine Colostrums on Human Leukemic U937 Cells

Growth Inhibition and Differentiating Effects of Protein Hydrolysates From Bovine Colostrums on Human Leukemic U937 Cells

Effects of vinegar–egg on growth inhibition, differentiation human leukemic U937 cells and its immunomodulatory activity

In Vitro Antitumor and Immunomodulatory Effects of the Protein PCP-3A from Mushroom Pleurotus citrinopileatus

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