Growth Inhibition and Differentiating Effects of Protein Hydrolysates From Bovine Colostrums on Human Leukemic U937 Cells

Chen, Chih‐Wei; Chiang, Shu-Hua; Wang, Shiu-Yu; Lin, You-Tung; Chang, Ching-Yu

doi:10.1111/j.1745-4514.2011.00590.x

Cited by 6 publications

(14 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bovine colostrum is the first milk produced postpartum and is typically defined as the first six postpartum milkings collected during the period of transition from colostrums to milk [ 1 , 2 ]. Several researchers have compared the composition of colostrums with those of mature milk and concluded that colostrums have higher levels of protein, lower levels of fat, a lactose solution rich in immunoglobulins, and other important immune elements and mediators [ 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…The minor constituents include immunoglobulins (9%), bovine serum albumin (5.5%), lactoferrin, phospholipoproteins, bioactive factors, and enzymes [ 7 ]. Several functional and biological activities of bovine colostrum whey protein and whey protein hydrolysates have been reported, such as U937 cell growth inhibition and immunomodulatory activities [ 1 ], antioxidation activities [ 8 ], inhibition of angiotensin I-converting enzyme and downregulation of fatty acid synthesis in liver [ 7 ], and inhibition of DNA oxidative damage and LDL oxidation [ 2 ]. Protein hydrolysate is a mixture of proteoses, peptones, peptides, and free amino acids.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bovine Colostrum Whey Protein Hydrolysate Inhibits Cell DNA Damage and LDL Oxidation In Vitro

et al. 2017

Self Cite

View full text Add to dashboard Cite

Whey protein isolated from bovine colostrums collected on the second day postpartum was two-stage hydrolyzed by alcalase and flavourzyme. The whey hydrolysates were finally fractionated by ultrafiltration (UF) with a 10 kDa molecular weight (MW) cutoff membrane and subsequently used to evaluate the effect of whey protein hydrolysis on inhibition of DNA oxidative damage and low-density lipoprotein (LDL) oxidation in vitro. Results showed that whey hydrolysis exhibited not only higher inhibitory activities of oxidative damage of deoxyribose but also an inhibitory effect on the breakdown of supercoiled DNA into open circular DNA and linear DNA. The quantities of 8-hydroxy-2′-deoxyguanosine (8-OH-2′-dG) formed with the addition of whey hydrolysate protein, the hydrolysate fraction of MW >10 kDa, and the hydrolysate fraction of MW <10 kDa were 0.25, 0.06, and 0.09 μg/mL, respectively. The lag time of conjugated diene formation of the control sample, which was only combined with cupric ions and LDL, was 90 min. The samples added with the hydrolysate fractions exhibited higher inhibitory activity on LDL oxidation. The whey hydrolysate fractions extended the lag time of conjugated diene formation to 270 min. The lag time of the whey hydrolysate fractions was 3 times that of the control.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bovine Colostrum Whey Protein Hydrolysate Inhibits Cell DNA Damage and LDL Oxidation In Vitro

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Bovine colostrums is the first milk produced postpartum and is typically defined as the first six postpartum milking collected during the period of transition from colostrums to milk [7,8]. Several researchers have compared the composition of colostrums with those of mature milk and concluded that colostrums have higher levels of protein, lower levels of fat, a lactose solution rich in immunoglobulins, and other important immune elements and mediators [9,10,11].…”

Section: Introductionmentioning

confidence: 99%

The Inhibition Effect of Cell DNA Oxidative Damage and LDL Oxidation by Bovine Colostrums

2016

Self Cite

View full text Add to dashboard Cite

Abstract:In the present study, we investigated the effect of bovine colostrums on inhibition of DNA oxidative damage and low density lipoprotein (LDL) oxidation in vitro. Results showed that whey and skimmed milk exhibited not only higher inhibitory activities of oxidative damage of deoxyribose but also an inhibitory effect on the breakdown of supercoiled DNA into open circular DNA and linear DNA. The quantities of 8-OH-2 -dG formed under whey, caseins and skimmed milk treatment were 0.24, 0.24 and 1.24 µg/mL, respectively. The quantity of malondialdehyde formed through LDL oxidation induced by copprous ion was significantly decreased as colostrums protein solutions were added, in which whey and caseins led to a more significant decrease than skimmed milk. The formation of conjugated dienes could be inhibited by treatment with colostrums protein solutions. Whey exhibited the longest lag time of conjugated dienes formation among the colostrums proteins. The lag time of the whey was 2.33 times that of the control. From the results of foregoing, the bovine colostrums protein has potential value in the inhibition of DNA oxidation damage and LDL oxidation.

show abstract

“…A suspension of 5 × 10 5 cells/mL in an RPMI 1640 medium was prepared. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assays for determining the growth of MNCs were modified from those of Liao et al [ 26 ] and Chen et al [ 27 ]. MNCs were incubated with various concentrations of different T. triangulare extracts in medium, medium only, and medium with same extract volume of phosphate buffer saline (PBS; control) at 37°C in a humidified atmosphere containing 5% CO 2 for 24 and 72 hours, respectively.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant activities and contents of flavonoids and phenolic acids of Talinum triangulare extracts and their immunomodulatory effects

Liao

Chai

Wang

et al. 2015

Journal of Food and Drug Analysis

View full text Add to dashboard Cite

In this study, leaves and stems of Talinum triangulare were sequentially extracted with phosphate buffer solution to obtain PTL and PTS (phosphate buffered extracts of T. triangulare leaves and stems), with 75% ethanol to obtained ETL and ETS (ethanol extracts of T. triangulare leaves and stems), or with 90°C boiling water to obtain WTL and WTS (water extracts of T. triangulare leaves and stems). We investigated the antioxidant activities of various T. triangulare extracts, analyzed the extracts' stimulations on human mononuclear cell (MNC) growth and secretion of cytokines (interleukin-1 beta, interferon-γ, and tumor necrosis factor-α) and nitric oxide, and then assayed their subsequent inhibitions on human leukemic U937 cell growth. Results indicated that extracts of T. triangulare showed significant antioxidant activities. Among these extracts, WTS showed the highest stimulatory effect on human MNC growth. The secretion levels of interleukin-1 beta, interferon-γ, and tumor necrosis factor-α in the conditioned medium, wherein human MNC was treated with 500 μg/mL WTS for 72 hours, were 1275, 859, and 2222 pg/mL, respectively. All conditioned media obtained from human MNCs cultured with various T. triangulare extracts showed significant inhibition against U937 cell growth of over 40%. These results suggest that T. triangulare extracts may be used in health foods for their immunomodulatory potential.

show abstract

Growth Inhibition and Differentiating Effects of Protein Hydrolysates From Bovine Colostrums on Human Leukemic U937 Cells

Cited by 6 publications

References 29 publications

Bovine Colostrum Whey Protein Hydrolysate Inhibits Cell DNA Damage and LDL Oxidation In Vitro

Bovine Colostrum Whey Protein Hydrolysate Inhibits Cell DNA Damage and LDL Oxidation In Vitro

The Inhibition Effect of Cell DNA Oxidative Damage and LDL Oxidation by Bovine Colostrums

Antioxidant activities and contents of flavonoids and phenolic acids of Talinum triangulare extracts and their immunomodulatory effects

Contact Info

Product

Resources

About