Effect of calcium-binding peptide from Pacific cod (Gadus macrocephalus) bone on calcium bioavailability in rats

Peng, Zhe; Hou, Hu; Zhang, Kai; Li, Bafang

doi:10.1016/j.foodchem.2016.10.078

Cited by 102 publications

(69 citation statements)

References 36 publications

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“…Amino acids are known to enhance calcium absorption via the Na + ‐dependent mechanism. Moreover, it was recently reported that rats given calcium chelated with peptides from fish bone exhibited higher fractional intestinal calcium absorption and calcium retention than those given purified CaCO 3 …”

Section: Discussionmentioning

confidence: 99%

Fortified tuna bone powder supplementation increases bone mineral density of lactating rats and their offspring

2017

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Section: Discussionmentioning

confidence: 99%

Fortified tuna bone powder supplementation increases bone mineral density of lactating rats and their offspring

2017

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“…In the recent decade, calcium binding peptides and the peptide-calcium complex have attracted great attention and many food proteins have been explored to prepare and isolate calcium binding peptides, including tilapia protein [ 10 ], Antarctic krill protein [ 11 ], Pacific cod skin protein [ 12 ], whey protein [ 13 ], duck egg white [ 14 ], etc. Furthermore, previous studies reported that fishbone derived peptides also possess high calcium binding activity and can improve calcium bioavailability [ 15 , 16 ]. Thus, this provides a possibility for their anti-osteoporosis activities [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, it contains a high protein content (about 30 g protein per 100 g dry fishbone) [ 19 ]. This makes fishbone an excellent resource of proteins and peptides [ 16 , 18 ]. Therefore, many researches have been devoted to the utilization of fishbone derived bioactive peptides [ 18 ], especially the calcium binding peptides and peptide-calcium complex [ 8 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Calcium Binding Peptides from Pacific Cod (Gadus macrocephalus) Bone: Calcium Bioavailability Enhancing Activity and Anti-Osteoporosis Effects in the Ovariectomy-Induced Osteoporosis Rat Model

Zhang

Chen

et al. 2018

Nutrients

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Calcium binding peptides from Pacific cod (Gadus macrocephalus) bone have attracted attention due to their potential effects on bone health. In this study, calcium binding peptides (CBP) were prepared from Pacific cod bone by trypsin and neutral protease. Ultraviolet spectra, circular dichroism (CD), and Fourier transform infrared spectroscopy (FTIR) revealed that carboxyl and amino groups in CBP could bind to Ca2+, and form the peptide-calcium complex (CBP-Ca). Single-pass intestinal perfusion (SPIP) experiments indicated that the intestinal calcium absorption was significantly enhanced (p < 0.01) in CBP-Ca treated Wistar rats. The anti-osteoporosis activity of CBP-Ca was investigated in the ovariectomized (OVX) Wistar rat model. The administration of CBP-Ca significantly (p < 0.01) improved the calcium bioavailability, trabecular bone structure, bone biomechanical properties, bone mineral density, and bone mineralization degree. CBP-Ca notably (p < 0.01) increased serum calcium, however, it remarkably (p < 0.01) reduced the levels of osteocalcin (OCN), bone alkaline phosphatase (BALP), tartrate-resistant acid phosphatase isoform 5b (TRAP5b), and C-telopeptide of type I collagen (CTX-1) in serum. Results suggested that the cod bone derived CBP could bind with calcium, improve the intestinal calcium absorption, calcium bioavailability, and serum calcium, then reduce the bone turnover rate, and thus ameliorate osteoporosis.

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“…Secondly, the added Ca 2+ shielded the negative charges on polypeptide chains and formed a "salt bridge" to induce protein aggregation (Chen et al, 2014). The similar phenomenon was also observed by (Peng et al, 2017). All of the structural changes between the DBP and the DBPCC proved that the DBP chelated with Ca 2+ to form the DBPCC, so the DBP and the DBPCC were two completely different substances.…”

Section: Chemical Analysismentioning

confidence: 64%

Preparation and Characterization for Peptide-Chelated Calcium of Deer Bone

Wang

Zhou

et al. 2018

FSTR

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The deer bone peptides (DBP) was used to chelate with Ca 2+ to form the deer bone peptides chelated calcium (DBPCC), which could be a calcium nutraceutical supplement. The optimal condition for the chelation reaction was that 1.535 mg mL _ 1 DBP reacted with 7.5 mmol L _ 1 CaCl 2 at pH 7.0 and 40 ℃ for 15 min. Negatively charged amino acids, positively charged amino acids and hydrophobic amino acids in the DBPCC were probably important to the chelation reaction. And the DBP could chelate with Ca 2+ through metal-binding sites (carboxyl oxygen atoms and amino nitrogen atoms). The Ca 2+ would cause the DBP folding and conformation modification. Furthermore, the DBPCC was stable under ileum conditions (pH 7.0, 37 ℃), indicating that it would be well absorbed in the human body and had the potential to improve the traditional calcium nutrition fortifier.

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Effect of calcium-binding peptide from Pacific cod (Gadus macrocephalus) bone on calcium bioavailability in rats

Cited by 102 publications

References 36 publications

Fortified tuna bone powder supplementation increases bone mineral density of lactating rats and their offspring

Fortified tuna bone powder supplementation increases bone mineral density of lactating rats and their offspring

Functional Calcium Binding Peptides from Pacific Cod (Gadus macrocephalus) Bone: Calcium Bioavailability Enhancing Activity and Anti-Osteoporosis Effects in the Ovariectomy-Induced Osteoporosis Rat Model

Preparation and Characterization for Peptide-Chelated Calcium of Deer Bone

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