Effect of Calcium Hydroxide-Propolis Combination  on the Number of Fibroblas Cells and Angiogenesis in Wistar Rats Pulp

Pribadi, Nirawati; Widjiastuti, Ira; Nadia, Agata

doi:10.20473/cdj.v10i1.2020.14-18

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…Topoisomerase is an enzyme that plays important role in chromosome transcription, replication and has the ability to regulate the superhelical area of chromosomal DNA. Therefore, propolis does not cause DNA damage (28) . Because propolis has a potential cytotoxic effect on various cancer cell lines, it was necessary to investigate its cytotoxicity in stem cells.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic Effect of Different Concentrations of Chitosan and Propolis Nanoparticles on Periodontal Ligament Stem Cells

et al. 2022

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Purpose:The purpose of this study was to assess the cytotoxic effect of different concentrations of Chitosan Nanoparticles (CNPs) and Propolis Nanoparticles (PNPs) on periodontal ligament stem cells using MTT assay. Materials and Methods:Immature, impacted third molars were used to isolate stem cells. Cells were seeded in 96-well culture plates. Groups were divided into six experimental groups: Group I: 17% EDTA, Group II: 1% propolis NPs, Group III: 2% propolis NPs, Group IV: 1% chitosan NPs, Group V: 2% chitosan NPs, Group VI: cultured cells not receive any material. Cytotoxic effect of these materials on stem cells was evaluated using MTT assay at 24, 72,120 hours and 14 days. Results: In all assay intervals; 2%CNPs group showed the statistically significant highest median value and control group showed the statistically significant lowest median values. After 24 and 72 hours; there was no statistically significant difference between 2% PNPs and 1% CNPs groups followed by 1% PNPs group. Both EDTA and control groups; showed the statistically significantly lowest median values with no statistically significant difference between them. After 120 hours; there was no statistically significant difference between 2% PNPs and 1% CNPs groups in median value followed by 1% PNPs then EDTA groups. After 14 days; there was no statistically significant difference between 1% PNPs, 2% PNPs and 1% CNPs groups in median value followed by EDTA group. Conclusion: The ability of 2% Chitosan NPs to promote periodontal ligament stem cell viability makes them a promising irrigating solution. The cell viability of 2% Propolis NPs was comparable to that of 1% Chitosan NPs, which was better than EDTA.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic Effect of Different Concentrations of Chitosan and Propolis Nanoparticles on Periodontal Ligament Stem Cells

et al. 2022

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Inhibitory effect of nano Stolephorus insularis and calcium hydroxide on glucosyltransferase (GTF) activity of Lactobacillus aciophilus

Yuanita

Juniarti

Marbun

2023

CDJ

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Background: Dental caries is the most common multifactorial infectious disease worldwide which referes to the process of destruction tooth hard tissue caused by bacterial by-products in the form of acids through carbohydrate fermentation. Lactobacillus acidophilus is one of the main cariogenic bacteria that causes caries by forming biofilms with the help ofglucosyltransferase (GTF) enzymes. Calcium hydroxide has limited antibacterial effects. Nano brown anchovy contains fluor as the active compound which has the potential as an antibacterial alternative agent. Purpose: To explain the inhibition of the combination of nano Stolephorus insularis and calcium hydroxide on the activity of the glucosyltransferase enzyme of Lactobacillus acidophilus bacteria. Methods: This study used a combination of nano Stolephorus insularis 3.125% with calcium hydroxide as a treatment and aquades as a control. The GTF enzyme was obtained from the supernatant centrifuged by Lactobacillus acidophilus in BHIB. The activity of the GTF enzyme was considered by calculating the fructose levels using High Performance Liquid Chromatography (HPLC) in a certain formula.Results: Decreased levels of fructose was obtained in the treatment group. From the results of Mann- Whitney data analysis, there were significant difference in the study groups. Conclusion: the combination of nano Stolephorus insularis with calcium hydroxide is effective to inhibit the glucosyltransferase enzyme activity of Lactobacillus acidophilus bacteria.

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Effect of Calcium Hydroxide-Propolis Combination on the Number of Fibroblas Cells and Angiogenesis in Wistar Rats Pulp

Cited by 2 publications

References 10 publications

Cytotoxic Effect of Different Concentrations of Chitosan and Propolis Nanoparticles on Periodontal Ligament Stem Cells

Cytotoxic Effect of Different Concentrations of Chitosan and Propolis Nanoparticles on Periodontal Ligament Stem Cells

Inhibitory effect of nano Stolephorus insularis and calcium hydroxide on glucosyltransferase (GTF) activity of Lactobacillus aciophilus

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