Effect of carbon source and concentration on the molecular mass of poly(3-hydroxybutyrate) produced by Methylobacterium extorquens and Alcaligenes eutrophus

Taidi, Behnam; Anderson, Angelika; Dawes, E. A.; Byrom, David

doi:10.1007/bf00173975

Cited by 83 publications

(44 citation statements)

References 24 publications

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“…This result indicates that the PHB produced by the glycerolsupplemented cultures had much lower Mn and Mw than the cultures grown in glucose. Taidi et al also reported a decrease in the molecular weight of the PHB production when glycerol was used as the sole carbon source, 10) as it reacts as end-cap to stop polymerization. 11) In future studies, the composition of the medium and the growth conditions used should be modified to obtain high molecular weight PHB for practical applications.…”

mentioning

confidence: 99%

Poly(3-hydroxybutyrate) Production by IsolatedHalomonassp. KM-1 Using Waste Glycerol

Kawata

Aiba²

2010

Bioscience, Biotechnology, and Biochemistry

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Biodiesel fuel is favored as a type of carbon neutral energy. To popularize its usage, by-product waste glycerol utilization is a critical problem. We tried to isolate waste glycerol utilizing bacteria, and obtained the alkalo-and halophile bacteria Halomonas sp. KM-1. This strain produced bioplastic poly(3-hydroxybutyrate) (PHB) in a simple medium and diluted waste glycerol as a sole carbon source.

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confidence: 99%

Poly(3-hydroxybutyrate) Production by IsolatedHalomonassp. KM-1 Using Waste Glycerol

Kawata

Aiba²

2010

Bioscience, Biotechnology, and Biochemistry

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“…The glycerol utilization as a carbon source was reported by Taidi et al for Ralstonia. eutroph (Taidi et al., 1994). The culture showed no difference in the PHB production in media containing sucrose and glycerol as carbon source.…”

Section: Evaluation Of Agro-industrial Residues As Carbon Substratementioning

confidence: 85%

Polyhydroxybutyrate production using agro-industrial residue as substrate by Bacillus sphaericus NCIM 5149

Ramadas

Singh

Soccol

et al. 2009

Braz. arch. biol. technol.

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The aim of this work was to study the production of polyhydroxybutyrate (PHB) using agro-industrial residues as the carbon source. Seven substrates, viz., wheat bran, potato starch, sesame oil cake, groundnut oil cake, cassava powder, jackfruit seed powder and corn flour were hydrolyzed using commercial enzymes and the hydrolyzates assessed for selecting the best substrate for PHB production. Jackfruit seed powder gave the maximum production of PHB under submerged fermentation using Bacillus sphaericus (19%) at the initial pH of 7.5.

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“…In order to understand the function of phasins, we have generated new tools and carried out a systematic study to examine the kinetics and amounts of the Ralstonia eutropha phasin PhaP in R. eutropha under a variety of growth conditions. R. eutropha is well suited for studies on phasins, given that the strain produces a PHA, poly-[(R)-3-hydroxybutyrate] (PHB), under many standard cultivation conditions (5,17) and is amenable to genetic manipulation (8,12,14). The first genetic analysis of the role of PhaP in PHB synthesis in R. eutropha was conducted by Wieczorek et al (18).…”

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New Insight into the Role of the PhaP Phasin of Ralstonia eutropha in Promoting Synthesis of Polyhydroxybutyrate

York¹,

Stubbe

Sinskey³

2001

J Bacteriol

122

145

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Phasins are proteins that are proposed to play important roles in polyhydroxyalkanoate synthesis and granule formation. Here the phasin PhaP of Ralstonia eutropha has been analyzed with regard to its role in the synthesis of polyhydroxybutyrate (PHB). Purified recombinant PhaP, antibodies against PhaP, and an R. eutropha phaP deletion strain have been generated for this analysis. Studies with the phaP deletion strain show that PhaP must accumulate to high levels in order to play its normal role in PHB synthesis and that the accumulation of PhaP to low levels is functionally equivalent to the absence of PhaP. PhaP positively affects PHB synthesis under growth conditions which promote production of PHB to low, intermediate, or high levels. The levels of PhaP generally parallel levels of PHB in cells. The results are consistent with models whereby PhaP promotes PHB synthesis by regulating the surface/volume ratio of PHB granules or by interacting with polyhydroxyalkanoate synthase and indicate that PhaP plays an important role in PHB synthesis from the early stages in PHB production and across a range of growth conditions.Polyhydroxyalkanoates (PHAs) are polyoxoesters that are synthesized intracellularly in diverse bacteria under conditions of limitation for a nutrient other than carbon (5). The polymers are water insoluble and accumulate as intracellular granules (5). Phasins are low-molecular-weight proteins that are proposed to promote PHA synthesis in cells (9,10,15,18). Three different mechanisms for the function of phasins have been proposed. First, phasins may enhance PHA production by binding to granules and increasing the surface/volume ratio of the granules (18). Second, phasins may activate the rate of PHA synthesis by interacting directly with PHA synthase (18). Third, phasins may promote PHA synthesis indirectly by preventing growth defects associated with the binding of other cellular proteins to PHA granules (6, 18). Phasins have also been proposed to function as storage proteins (7), a role that could also conceivably affect PHA synthesis. Studies on the function of phasins in recombinant host strains (6, 9) and in vitro (3) have yielded anomalous results which hint that the timing and levels of expression of phasins may be crucial for their function. In order to understand the function of phasins, we have generated new tools and carried out a systematic study to examine the kinetics and amounts of the Ralstonia eutropha phasin PhaP in R. eutropha under a variety of growth conditions. R. eutropha is well suited for studies on phasins, given that the strain produces a PHA, poly-[(R)-3-hydroxybutyrate] (PHB), under many standard cultivation conditions (5, 17) and is amenable to genetic manipulation (8,12,14). The first genetic analysis of the role of PhaP in PHB synthesis in R. eutropha was conducted by Wieczorek et al. (18). They reported the isolation of five phaP::Tn5 mutants that were claimed to be defective in the production of PhaP and to produce half as much PHB as the wild-type (wt) strain (18...

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Effect of carbon source and concentration on the molecular mass of poly(3-hydroxybutyrate) produced by Methylobacterium extorquens and Alcaligenes eutrophus

Cited by 83 publications

References 24 publications

Poly(3-hydroxybutyrate) Production by IsolatedHalomonassp. KM-1 Using Waste Glycerol

Poly(3-hydroxybutyrate) Production by IsolatedHalomonassp. KM-1 Using Waste Glycerol

Polyhydroxybutyrate production using agro-industrial residue as substrate by Bacillus sphaericus NCIM 5149

New Insight into the Role of the PhaP Phasin of Ralstonia eutropha in Promoting Synthesis of Polyhydroxybutyrate

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