Effect of cationic cholesterol derivatives on gene transfer and protein kinase C activity

Lipofection of cultured mouse muscle cells: a direct comparison of Lipofectamine and DOSPER

“…This contrasted with Lipofectamine which, unless used in serum-free medium, was unable to induce any gene transfer. This phenomenon of inactivation in the presence of serum has previously been reported for other cationic lipids 24 and is likely to be due to the neutralising effect of anionic components of the serum. Although Lipofectamine promotes transfection at a higher efficiency relative to DOSPER in serum-free medium, this can be considered an artificial environment.…”

Polyion complex micelles as vectors in gene therapy – pharmacokinetics and in vivo gene transfer

“…The protocol with lipofection seems to be most promising, 20 but this also has some problems involving cytotoxicity. 21 The approach employing PIC is an attractive alternative for targeted gene delivery systems. Intravenous administration of polymer particles is often accompanied by interactions with blood components such as IgM, fibrinogen, fibronectin and complement C3.…”

In vivo gene transfer via intravenous administration of cationic lipid–protamine–DNA (LPD) complexes

“…Viral vectors, although highly ship of the cationic lipids. [15][16][17] For example, transfection efficient, suffer from a number of problems such as efficiency can be improved significantly by designing and immunogenicity, 3 toxicity 4 and potential recombination synthesizing new lipids. 17,18 Cationic lipid-DNA comor complementation.…”