Effect of cellular products of potential probiotic bacteria on the immune response of Labeo rohita and susceptibility to Aeromonas hydrophila infection

Giri, Sib Sankar; Sen, Shib Sankar; Chi, Cheng; Kim, Hyoun Joong; Yun, Sukyoung; Park, Taesung; Sukumaran, V.

doi:10.1016/j.fsi.2015.08.012

Cited by 50 publications

(44 citation statements)

References 39 publications

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“…In Asia, disease caused by A. hydrophila is most widespread bacterial diseases in freshwater fish, and A. hydrophila infection may result in increased mortality in fishery products (Giri et al, 2015;Ramesh et al, 2015). In this study, a pathogenetic bacterial strain SJ-2 was isolated from the diseased mussel and the strain was basically consistent with the genus Aeromonas further confirmed by biological characteristics (Canals et al, 2006;Hussain et al, 2014).…”

Section: Discussionsupporting

confidence: 52%

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Zhong¹,

Xu²,

Yan³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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To perform pathogenic and pathological investigations on explosive epidemics of Hyriopsis cumingii Lea in Hunan Province, China between 2005 and 2011. We isolated pathogenic bacteria SJ-2 from naturally infected Hyriopsis cumingii Lea, and the SJ-2 strain was artificial infected into the normal Hyriopsis cumingii Lea by axe foot injection method. The strain was identified according to conventional bacteriological isolation and identification and molecular biology methods, and pathological changes caused by the bacteria in diseased mussel tissues were observed. According to morphological and culture characteristics, physiological and biochemical tests and 16S rDNA molecular identification, SJ-2 strain shares 99% sequence identity with the type strain of Aeromonas veronii (A. Veronii ). Results for antibiotic susceptibility tests showed that among 50 drugs, A. Veronii was highly sensitive to 15 drugs, moderately sensitive to 5 drugs, and showed drug resistance to left drugs. Macroscopical lesions, pathological changes and cytopathology were summarized. The pathogenic bacteria SJ-2 was isolated from Hyriopsis cumingii Lea affected by explosive epidemic disease, and was identified as A. Veronii. SJ-2 strain caused multi-organ lesions and function failure in corresponding organs in the Hyriopsis cumingii Leal, resulting in gradually decreased and disappeared normal physiological metabolism, and eventually death.

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Section: Discussionsupporting

confidence: 52%

Untitled

Zhong¹,

Xu²,

Yan³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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“…Lysozyme has substantial bactericidal activity against both gram-positive and gram-negative bacteria in the presence of complement (Giri et al, 2015). In fish, complement alarms the host immune system to the existence of potential pathogens and assists in their subsequent clearance (Holland and Lambris, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…‘Blood samples were collected from the caudal vein using a 2-mL syringe after anesthetisation with MS222 (Sigma-Aldrich, St. Louis, MO, USA) and transferred to centrifuge tubes (Eppendorf, Germany).’ Serum was collected by centrifugation (4,000 × g , 10 min, 4°C) and stored at 4°C until use’ (Giri et al, 2015). Intestinal samples from the fish were dissected aseptically, rinsed with cold distilled water, and used for the analysis of intestinal enzyme activities.…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA concentration and purity were determined using a spectrophotometer, and the quality was checked by agarose gel electrophoresis. The extracted RNA was treated with RNA-Free DNase (Takara, Shiga, Japan) to remove contaminating DNA and then reverse transcribed into cDNA using a Superscript cDNA synthesis kit (Life Technologies) following the manufacturer’s instructions” (Giri et al, 2015). Real-time PCR analysis of TNF-α, IL-1β, IL-10, IL-12, TGF-β, NF-κB p65, IKK-β, IκB-α, and β-actin mRNA expression was performed using the CFX96 TM Real-Time PCR platform (Bio-Rad) following standard protocols with the primers and thermo-cycling conditions indicated in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

“…β-Actin was used as an endogenous control for data analysis. All samples were run in parallel with the housekeeping gene, which was used to normalize cDNA loading” (Giri et al, 2015). Gene expression results were analyzed using the 2 -ΔΔCT method after verification that the primers amplified with an efficiency of approximately 100% (Livak and Schmittgen, 2001); data for all treatment groups were compared to those of the control group.…”

Section: Methodsmentioning

confidence: 99%

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Role of Bacillus licheniformis VS16-Derived Biosurfactant in Mediating Immune Responses in Carp Rohu and its Application to the Food Industry

et al. 2017

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Multifarious applications of Bacillus licheniformis VS16-derived biosurfactant were explored. Labeo rohita fingerlings were injected intraperitoneally with 0.1 mL of phosphate-buffered saline (PBS) containing purified biosurfactant at 0 (control), 55 (S55), 110 (S110), 220 (S220), or 330 (S330) μg mL-1 concentrations. Various immunological parameters and the expression of immune-related genes were measured at 7, 14, and 21 days post-administration (dpa). At 21 dpa, fish were challenged with Aeromonas hydrophila and mortality was recorded for 14 days. Immune parameters such as lysozyme levels (39.29 ± 2.14 U mL-1), alternative complement pathway (61.21 ± 2.38 U mL-1), and phagocytic activities (33.37 ± 1.2%) were maximum (P < 0.05) in the S220 group at 14 dpa; but immunoglobulin levels (11.07 ± 0.83 mg mL-1) were highest in the S220 group at 7 dpa, compared to that in controls. Activities of digestive enzymes (amylase, protease, and lipase) were higher (P < 0.05) in the S220 and S330 groups than in the control group. Regarding cytokine gene expression, pro-inflammatory cytokines (TNF-α and IL-1β) were down-regulated (P < 0.05) in the S220 and S330 groups. Expression of IL-10, TGF-β, and IKB-α were up-regulated in the S220 and S330 groups at 14 dpa, with the highest levels in the S220 group. The expression of NF-κB p65 and IKK-β were down-regulated in treatment groups, and were lowest (P < 0.05) in the S220 group. The highest post-challenge survival rate (72.7%) was recorded in S220 group. Further, the potential of this substance to inhibit biofilm formation, and heavy metal removal from vegetables were also evaluated. Biosurfactant was effective in inhibiting biofilm formation up to 54.71 ± 1.27%. Moreover, it efficiently removed cadmium (Cd) from tested vegetables such as carrot, radish, ginger, and potato, with the highest removal efficiency (60.98 ± 1.29%) recorded in ginger contaminated with Cd. Collectively, these results suggest that isolated biosurfactant could be used in the aquaculture industry, in addition to its potential application to the food industry.

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