Effect of chloride and diamide on serum angiotensin i-converting enzyme activity from eight mammalian species

Ibarra-Rubio, María Elena; Gómez-Velez, Luis Angel; Cruz, Cristino; Pedraza‐Chaverrí, José

doi:10.1016/0742-8413(93)90169-l

Cited by 1 publication

(2 citation statements)

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“…10 μl of 100 mM diamide (Prepared in 0.1M phosphate buffer pH 8.3 ) were added to 90 μl of tissue extract or serum to give 10mM finalconcentration (6).10μl of deionized water were added to blank tubes. Tissue extracts and serum were chilled for 30 minutes at 0°C and used for determination of ACE activity.…”

Section: Methodsmentioning

confidence: 99%

“…The mechanism by which chloride activates ACE has not been established clearly (5). Further,effect of chloride on serum ACE from eight mammalian species with varried ACE activity showed differences in optimum chloride concentration among species (6). Relationship between difference in ACE activity among tissues and chloride dependence is unknown in any animal species.…”

Section: Introductionmentioning

confidence: 99%

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Effect of chloride and diamide on sheep kidney, lung and serum angiotensin converting enzyme

Rao¹,

Udupa²

2008

Indian J Clin Biochem

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The effect of chloride and diamide on angiotensin converting enzyme (ACE) activity from sheep kidney, lung and serum was investigated by using Hip-His-Leu as substrate. Optimum chloride concentrations were 400 -1000 mM for kidney, 700 mM for lung and 1000 mM for serum. Optimum chloride concentration increased ACE activity of serum and lung 1.70 folds and 2.73 folds respectively of the activity at physiological chloride concentrations, suggesting that the effect of salt on blood pressure may be due to the chloride sensitivity of ACE. The difference in effect of chloride on lung, kidney and serum ACE suggest that each tissue ACE has unique three dimentional structure. Increased pulmonary and serum ACE activity on pretreatment with diamide indicates that tissue oxidation may alter blood pressure.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%