Objective: To evaluate the effect of cigarette smoking and pack-year of cigarette smoking on red blood cell counts and its related parameters, also glucose concentration. Methods: Sixty consenting subjects of which 30 controls (non-smokers) and 30 cases (smokers) were studied. Smokers were divided into two groups based on pack-year of cigarettes smoked. The red blood cell parameters and glucose level were analyzed in smoker and non-smoker. Independent T-test was used to comparison of a smoker and also one way ANOVA was used to comparison packyear of smoking groups.Results: The present study showed no changes significantly in red blood cell parameter and glucose level between in smoker. The current result appeared alteration of red blood cells counts (RBC), hemoglobin level (HGB), hematocrit percent (HCT) and mean corpuscular hemoglobin concentration level (MCHC), while no difference of mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), red cell distribution width (RDW) and glucose level in duration of smoking. Conclusions: This study revealed that cigarette smoking statistically no effect on RBC counts and its related parameters and glucose level among healthy. However, the duration of smoking statistically had the effect on RBC, HGB, HCT and MCHC while had no effect on MCV, MCH, RDW and glucose level.