Effect of Citronella Oil on Time Kill Profile, Leakage and Morphological Changes of<i>Propionibacterium acnes</i>

Lertsatitthanakorn, Pilanthana; Taweechaisupapong, Suwimol; Arunyanart, Channarong; Aromdee, Chantana; Khunkitti, Watcharee

doi:10.1080/10412905.2010.9700322

Cited by 18 publications

(12 citation statements)

References 23 publications

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“…Corresponding to other results that, eucalyptus oil is more active against gram-positive bacteria than gramnegative bacteria which is attributed to the major chemical constituents [29]. The main chemical compositions; citronellal, isopulegol and citronellol, might be the key chemical constituents which can cause the discharge of gram-negative outer membrane lipopolysaccharide and increase the permeability of the cytoplasmic membrane leading to cell wall lost rigid structure and cell wall components were ruptured [30]. SDS-PAGE is a powerful technique for demonstrating antimicrobial effectivity in bacteria [26,31].…”

Section: Discussionmentioning

confidence: 72%

Chemical Composition and Antimicrobial Activity of Essential Oil Extracted from Eucalyptus citriodora Leaf

Insuan¹,

Chahomchuen²

2020

Microbiology and Biotechnology Letters

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Eucalyptus oil is a rich source of bioactive compounds with a variety of biological activities and is widely used in traditional medicine. Eucalyptus citriodora is cultivated for the production of essential oils. However, the mode of antibacterial action of essential oils from E. citriodora is not well-known. This study aimed to determine the chemical components, microbial inhibitory effect, and mechanism of action of the essential oil from E. citriodora. The oil was extracted from E. citriodora leaves by hydro-distillation and the chemical components were analyzed using gas chromatography-mass spectrometry. The antibacterial activities of eucalyptus oil against gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, and Staphylococcus intermedius) and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) were screened by disc diffusion method and quantitative analysis was conducted by the microdilution method. The mechanism of action of the extracted essential oil was observed using SEM and analyzed by SDS-PAGE. The major components of E. citriodora oil were citronellal (60.55 ± 0.07%), followed by dl-isopulegol (10.57 ± 0.02%) and citronellol (9.04 ± 0.03%). The antibacterial screening indicated that E. citriodora oil exhibited prominent activity against all tested strains. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against B. subtilis were 0.5% and 1.0%, respectively. The MIC and MBC concentrations against S. aureus, S. intermedius, E. coli, and P. aeruginosa were 1% and 2%, respectively. As observed by SEM, the antibacterial mechanism of E. citriodora oil involved cell wall damage; SDS-PAGE revealed decrease in protein bands compared to untreated bacteria. Thus, E. citriodora oil showed significant antimicrobial properties and caused cellular damage.

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Section: Discussionmentioning

confidence: 72%

Chemical Composition and Antimicrobial Activity of Essential Oil Extracted from Eucalyptus citriodora Leaf

Insuan¹,

Chahomchuen²

2020

Microbiology and Biotechnology Letters

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“…Agar diffusion assay was performed to determine the antimicrobial activities of the extracts as described by Clinical and Laboratory Standards Institute (CLSI) with some modifications [6,39]. Briefly, P. acnes suspension was diluted with RCM to a final concentration of approximately 1 × 10 6 CFU/mL (Optical density; OD600 = 0.1).…”

Section: Disc Diffusion Methodsmentioning

confidence: 99%

Rosa davurica Pall. Improves Propionibacterium acnes-Induced Inflammatory Responses in Mouse Ear Edema Model and Suppresses Pro-Inflammatory Chemokine Production via MAPK and NF-κB Pathways in HaCaT Cells

Hwang

Lee²,

Koh

et al. 2020

IJMS

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Acne, also known as acne vulgaris, is a common disorder of human skin involving the sebaceous gland and Propionibacterium acnes (P. acnes). Although there are a number of treatments suggested for acne, many of them have limitations in their safety and have efficacy issues. Therefore, there is a high demand to develop safe and effective novel acne treatments. In the present study, we demonstrate the protective effects of Rosa davurica Pall. leaves (RDL) extract against P. acnes-induced inflammatory responses in vitro and in vivo. The results showed that RDL dose-dependently inhibited the growth of skin bacteria, including P. acnes (KCTC3314) and aerobic Staphylococcus aureus (KCTC1621) or Staphylococcus epidermidis (KCTC1917). The downregulation of proinflammatory cytokines by RDL appears to be mediated by blocking the phosphorylations of mitogen-activated protein kinase (MAPK) and subsequent nuclear factor-kappa B (NF-κB) pathways in P. acnes-stimulated HaCaT cells. In a mouse model of acne vulgaris, histopathological changes were examined in the P. acnes-induced mouse ear edema. The concomitant intradermal injection of RDL resulted in the reduction of ear swelling in mice along with microabscess but exerted no cytotoxic effects for skin cells. Instrumental analysis demonstrated there were seven major components in the RDL extract, and they seemed to have important roles in the anti-inflammatory and antimicrobial effects of RDL. Conclusively, our present work showed for the first time that RDL has anti-inflammatory and antimicrobial effects against P. acnes, suggesting RDL as a promising novel strategy for the treatment of acne, including natural additives in anti-acne cosmetics or pharmaceutical products.

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“…Bakteri Propioni cane merupakan bakteri penyebab jerawat yang banyak tumbuh di kulit wajah. Lertsatitthanakorn et al (2010), mengemukakan minyak sereh wangi mampu merusak dinding sel, membran sitoplasma, dan material intraseluler dari bakteri Propioni acne. Kehadiran senyawa aldehid monoterpen dalam minyak sereh wangi yaitu citronelal, akan mengganggu nitrogen yang merupakan komponen protein pada membran sitoplasma, isi sitoplasma, dan asam nukleat.…”

Section: Sifat Antibakteri Minyak Sereh Wangiunclassified

“…Daya hambat terhadap pertumbuhan Pseudomonas aeruginosa lebih rendah dari hasil penelitian (Kamari et al 2018). Daya hambat minyak atsiri terhadap mikroba dipengaruhi oleh komponen senyawa penyusun minyak atsiri tersebut (Lertsatitthanakorn et al 2010). Perbedaan jenis dan kadar senyawa dalam minyak sereh wangi dipengaruhi oleh iklim, jenis tanaman dan metode penyulingan (Wu et al 2019).…”

Section: Sifat Antibakteri Minyak Sereh Wangiunclassified

Sifat Antimikroba dan Pengaruh Perlakuan Bahan Baku terhadap Rendemen Minyak Sereh Wangi (Antimicrobial Properties and Effects of Raw Material Treatments on Citronella Oil Yield)

Shintawati

Rina

Ermaya

2020

JSL

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Citronella oil is one of the non-timber forest products (NTFP) and commercially obtained from the distillation of the leaves of the Cymbopogon nardus L. This study aims to determine the effect of the size of the distillation raw material and storage time of the raw material on yield and determine the antimicrobial properties of citronella oil from plants in the production forest Register 40 KPH Gedong Wani, Lampung Selatan Regency. The research was conducted using a complete randomized block design (RAKL) with two factors, namely the size of the distillation raw material (whole leaf, 5 cm, 10 cm, and 15 cm) and the storage time of the raw material (fresh, 2 days, and 4 days) which was carried out in 3 replications. The variables measured were yield, specific gravity, and solubility of citronella oil in alcohol. The inhibition ability test of citronella oil against bacteria using essential oil obtained from distillation at the highest yield conditions, namely fresh leaves and leaf size of 5 cm using the diffusion well method. The results showed that the storage time factor, the size of the distillation raw material, and the interaction between the storage time and the size of the raw material had a significant effect on the yield of citronella oil. The highest yield of citronella oil was achieved in the condition of fresh leaves with a size of 5 cm, namely 2,09%. Density and solubility in alcohol of citronella oil produced met SNI 06-3953-1995 standard, respectively 0,8718-0,8928 g/ ml and 1:2. Citronella oil has strong antibacterial properties against Propioni acne, Escherichia coli, Staphylococcus aureus, and Bacillus cereus with inhibition zone diameters were 40,20 mm, 18,36 mm, 13,07 mm, and 18,80 mm, respectively. Citronella oil from plants in Register 40 KPH Gedong Wani has potential as a raw material for the cosmetic and disinfectant industry. Keywords: antimicrobial, citronella oil, yield

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Effect of Citronella Oil on Time Kill Profile, Leakage and Morphological Changes ofPropionibacterium acnes

Cited by 18 publications

References 23 publications

Chemical Composition and Antimicrobial Activity of Essential Oil Extracted from Eucalyptus citriodora Leaf

Chemical Composition and Antimicrobial Activity of Essential Oil Extracted from Eucalyptus citriodora Leaf

Rosa davurica Pall. Improves Propionibacterium acnes-Induced Inflammatory Responses in Mouse Ear Edema Model and Suppresses Pro-Inflammatory Chemokine Production via MAPK and NF-κB Pathways in HaCaT Cells

Sifat Antimikroba dan Pengaruh Perlakuan Bahan Baku terhadap Rendemen Minyak Sereh Wangi (Antimicrobial Properties and Effects of Raw Material Treatments on Citronella Oil Yield)

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