Effect of clonidine on blood glucose levels in euglycemic and alloxan-induced diabetic rats and its interaction with glibenclamide

Abstract

Objectives:Clonidine, a known antihypertensive, is currently used for many purposes including diabetic gastroparesis, postmenopausal hot flushes, opioid/nicotine/alcohol withdrawal. Its effects on carbohydrate metabolism appear to be variable. Hence, the present study was undertaken to evaluate the influence of clonidine on euglycemic and alloxan -induced diabetic rats and its interaction with glibenclamide.Materials and Methods:Alloxan - induced (150 mg/kg, i.p) diabetic rats were divided into six groups of six animals each. Group I - Normal Control; Group II - Nondiabetic + Clonidine (25 μg/kg); Group III - Diabetic Control; Group IV - Diabetic + Glibenclamide (5 mg/kg); Group V - Diabetic + Glibenclamide + Clonidine. All drugs were given orally once daily. Blood glucose was estimated from rat tail vein using glucometer before start of the experiment and at the end of 30 days.Results:After 30 days of treatment, clonidine (25 μg/kg) produced significant hyperglycemia in both euglycemic and diabetic rats. It also reduced the hypoglycemic effect of glibenclamide in diabetic rats.Conclusion:The results of present study indicate that clonidine has hyperglycemic effect and it also interacts with glibenclamide to reduce its hypoglycemic activity. If these findings are true to human beings then clonidine should not be used in diabetic patients on sulfonylureas.

Delayed gastric emptying in diabetic rats caused by decreased expression of cystathionine gamma lyase and H2S synthesis: in vitro and in vivo studies

“…To induce diabetes gastroparesis, overnight fasted rats were received a single intraperitoneally injection of alloxan monohydrate at 175 mg/kg. 9,[13][14] After 72 h, blood glucose was checked by glucometer (Elegance, Frankenberg, Germany). Rat with fasting blood glucose higher than 250 mg/dL was considered as diabetic.…”

Effect of tamsulosin on blood glucose levels in euglycemic and alloxan induced diabetic rats and its interaction with glibenclamide

“…Manjunath et al in their study carried on alloxan induced diabetic rats have concluded that clonidine has hyperglycemic effects and also interact with glibenclamide to reduce its hypoglycemic activity. 1 Clonidine is α2 receptor agonist and these authors have proposed that this hyperglycemia could be due to inhibition of insulin release, stimulation of growth hormone release and due to increased hepatic glycogenolysis by clonidine. In this study, we also found that tamsulosin interact with glibenclamide to reduce its hypoglycemic activity, however tamsulosin did not cause hyperglycemia in diabetic rats.…”

“…It is associated with many co-morbid conditions such as hypertension, obesity, cardiovascular disorder, dyslipidemia, mostly due to hyperglycemia caused by insulin deficiency or resistance to insulin. 1 In elderly male patients suffering from diabetes mellitus (DM), benign prostatic hyperplasia (BPH) is often associated for which α receptor antagonist such as prazosin, doxazosin, and selective α1A antagonist such as tamsulosin are frequently used. 2 Obesity, elevated blood glucose level and diabetes have been mentioned as risk factors for benign prostatic hyperplasia.…”

Evaluation of hypoglycemic activity of methanolic extract of Acorus calamus (linn). roots in alloxan induced diabetes rat model

“…17 The study was done in two phases. 12,13,16,18 In phase I, Oral Glucose Tolerance Test (OGTT) was done with 3 different doses of the methanolic extract of Acorus calamus (100, 150 and 200mg/kg).…”

“…Only those rats which developed FBS >250mg/dl after 7 days of alloxan administration were taken for study. 6,11,15,17,19 Rats were grouped as follows (Table 5). …”