Effect of colonic bypass with ileorectal anastomosis on plasma levels of SP and VIP in rats with slow transit constipation

Zhang, Weiping; Jiang, Bin; Huo, Mingdong; Ding, Shuqing; Wu, Yin-Ya; Xu, Shuai; Huangfu, Shaohua

doi:10.11569/wcjd.v20.i7.585

Cited by 2 publications

(2 citation statements)

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“…Constipation was induced in the animals by injecting 1 mL of 4 mg/kg loperamide twice a day for 3 days, while the control rats were administered normal saline [11]. Successful modeling was performed at day 0 (D0).…”

Section: Animal Experimental Designmentioning

confidence: 99%

“…The sections were dewaxed in xylene and then dehydrated in gradient ethanol. After rehydration, colon tissue sections were stained with HE according to the instructions of HE staining kit, and the morphological changes of colon tissue were observed under a Olympus BX60 research microscope (Olympus Corporation, Tokyo, Japan) [11].…”

Section: Histopathological Analysismentioning

confidence: 99%

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The water-eluting component of Anemarrhena asphodeloides Bge. laxative effects on loperamide-induced constipation rats

Li¹,

Liu²,

Sun³

et al. 2020

Preprint

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Background Slow transit functional constipation (STC) is the most common type of chronic constipation,with seriously affecting people's survival and quality of life in the recent years. Based on the safety and small side effects of traditional Chinese medicine, it is urgent to research and develop new medicine for the treatment of constipation. As a traditional Chinese medicine, Anemarrhena asphodeloides Bge. has been reported treat constipation in a long time, and carbohydrate is its active ingredient. Methods ELISA used for detection of serum gastrointestinal hormones and neurotransmitters levels. HE stain was analyzed to evaluate the colon injury. Western blot and RT-PCR detected interstitial cells of Cajal markers (SCF/c-Kit) and aquaporins (AQPs). Results The mainly compositions of oligosaccharide and amino acid from AABW was determined by HPLC. Based on the laxative activities of AABW in the study, AABW obviously enhanced the contents of VIP, Gas, MTL, SP and 5-HT and decreased the levels of NO from loperamide-induced rats by Elisa kits. Additionally, AABW could up-regulate the expressions of SCF, c-Kit, AQP3, VIP and down-regulate AQP8 to repair the damage of colon. Conclusion These findings suggested that AABW, which could treat constipation through mediating intestinal motility and water metabolism, were laxative active constituents from Anemarrhena asphodeloides Bge..

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Section: Animal Experimental Designmentioning

confidence: 99%

Section: Histopathological Analysismentioning

confidence: 99%