Effect of cooling and warming rate on glycerolized rabbit kidneys

Jacobsen, I.A.; Pegg, D.E.; Starklint, H; Chemnitz, J.; Hunt, Charles J.; Barfort, Per; Diaper, M.P.

doi:10.1016/0011-2240(84)90223-2

Cited by 51 publications

(7 citation statements)

References 28 publications

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“…22 Our results are encouraging, but indicate that ovarian function is compromised to some extent by freezing, perhaps because of intravascular ice formation, a problem that has previously frustrated attempts to cryopreserve kidney, but they were either unsuccessful or unrepeatable. 29 The disadvantage is that implantation of ovarian tissue without vascular reanastomosis leads to ischemia injury to the tissue and depletion of germ cells and their supporting somatic cells. Fortunately for the ovary, primordial follicles appear to be relatively resistant to ischemia, although losses always occur.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of vascularized ovary: An evaluation of histology and function in rats

et al. 2008

Microsurgery

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Cryopreservation of organs has been investigated to sustain the reproductive function of patients undergoing sterilizing chemotherapy and radiotherapy or reproductive surgery. A modified protocol for whole organ cryopreservation was described and the outcome of cryopreservative ovaries was evaluated, and apoptosis of cryopreservative cells stored for different time period and the viability of cryopreserved cells stored at different temperature was examined in rats. Lewis rat ovarian grafts were perfused for 30 min at 0.35 ml/min with M2 medium containing 0.1M fructose and increasing concentrations of 0-1.5M dimethylsulfoxide, cooled to -140 degrees C controlled by a computerized program, and stored in liquid nitrogen (-196 degrees C) for 24 hours. After being thawed, ovaries were transplanted to syngeneic recipients after bilateral oophorectomy. Graft functions were monitored postoperatively. The major findings were that: 1) A 100% survival rate of rat ovaries was achieved in this study. Ovarian hormone secretion recovered in 80% rats which had received cryopreservative ovarian grafts. Postoperative serum estradiol levels in the cryopreservative graft group were lower than in the sham surgery control, but much higher than in the bilateral oophorectomy group. 2) Histological examination of cryopreservative ovarian grafts showed preantral and antral follicles. Two gestations were obtained. 3) Estradiol levels remained low in ovariectomized rats while in the oophorectomized rats given cryopreservative ovarian grafts levels started to rise after 14 +/- 3 days. 4) The average viability in the cells from cryopreservative ovary organ (-196 degrees C) was about 71 +/- 18% compared to 90 +/- 9% of fresh cells. This success should encourage further improvement of cryopreservative techniques for large organs.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of vascularized ovary: An evaluation of histology and function in rats

et al. 2008

Microsurgery

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“…Most research effort has been focused on achieving the ultra-rapid cooling rate and lowering the concentration of cryoprotectant required for vitrification [18, 19, 25–27]. However, due to the low thermal conductivity of biological samples, the conventional approach of rewarming large-volume cryopreserved samples in a water bath is associated with non-uniform distribution of temperature, and this non-uniformity can induce thermal stress that can crack the brittle cryopreserved sample [28–30]. Moreover, a high heating rate for rewarming is crucial, because devitrification and recrystallization will occur if the temperature cannot be rapidly increased above the melting points of the aqueous sample.…”

Section: Introductionmentioning

confidence: 99%

Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification

Wang

Zhao

Zhang³

et al. 2016

Acta Biomaterialia

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Cryopreservation by vitrification has been recognized as a promising strategy for long-term banking of living cells. However, the difficulty to generate a fast enough heating rate to minimize devitrification and recrystallization-induced intracellular ice formation during rewarming is one of the major obstacles to successful vitrification. We propose to overcome this hurdle by utilizing magnetic induction heating (MIH) of magnetic nanoparticles to enhance rewarming. In this study, superparamagnetic (SPM) Fe3O4 nanoparticles were synthesized by a chemical coprecipitation method. We successfully applied the MIH of Fe3O4 nanoparticles for rewarming human umbilical cord matrix mesenchymal stem cells (hUCM-MSCs) cryopreserved by vitrification. Our results show that extracellular Fe3O4 nanoparticles with MIH may greatly suppress devitrification and/or recrystallization during rewarming and significantly improve the survival of vitrified cells. We further optimized the concentration of Fe3O4 nanoparticles and the current of an alternating current (AC) magnetic field for generating the MIH to maximize cell viability. Our results indicate that MIH in an AC magnetic field with 0.05% (w/v) Fe3O4 nanoparticles significantly facilitates rewarming and improves the cryopreservation outcome of hUCM-MSCs by vitrification. The application of MIH of SPM nanoparticles to achieve rapid and spatially homogeneous heating is a promising strategy for enhanced cryopreservation of stem cells by vitrification.

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“…There have been several hypotheses on the mechanisms of freezing-induced injury based upon a variety of factors [19,21], but experience with mammalian tissues shows that the disadvantages of classical cryopreservation revolve primarily around ice formation [22,23,37]. The formation of extracellular ice in particular (generally regarded as innocuous for cells in suspension) is known to be hazardous to structured tissues and organs [13,14,22,23,37]. …”

Section: Introductionmentioning

confidence: 99%

Thermal expansion of the cryoprotectant cocktail DP6 combined with synthetic ice modulators in presence and absence of biological tissues

2012

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This study explores physical effects associated with the application of cryopreservation via vitrification using a class of compounds which are defined here as synthetic ice modulators (SIMs). The general classification of SIMs includes molecules that modulate ice nucleation and growth, or possess properties of stabilizing the amorphous state, by virtue of their chemical structure and at concentrations that are not explained on a purely colligative basis. A subcategory of SIMs, referred to in the literature as synthetic ice blockers (SIBs), are compounds that interact directly with ice nuclei or crystals to modify their structure and/or rate of growth. The current study is part of an ongoing effort to characterize thermo-mechanical effects during vitrification, with emphasis on measuring the physical property of thermal expansion—the driving mechanism to thermo-mechanical stress. Materials under investigation are the cryoprotective agent (CPA) cocktail DP6 in combination with one of the following SIMs: 12% polyethylene glycol 400, 6% 1,3 cyclohexanediol, and 6% 2,3 butanediol. Results are presented for the CPA-SIM cocktail in the absence and presence of bovine muscle and goat artery specimens. This study focuses on the upper part of the cryogenic temperature range, where the CPA behaves as a fluid for all practical applications. Results of this study indicate that the addition of SIMs to DP6 allows lower cooling rates to ensure vitrification and extends the range of measurements. It is demonstrated that the combination of SIM with DP6 increases the thermal expansion of the cocktail, with implications for the likelihood of fracture formation—the most dramatic outcome of thermo-mechanical stress.

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Effect of cooling and warming rate on glycerolized rabbit kidneys

Cited by 51 publications

References 28 publications

Cryopreservation of vascularized ovary: An evaluation of histology and function in rats

Cryopreservation of vascularized ovary: An evaluation of histology and function in rats

Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification

Thermal expansion of the cryoprotectant cocktail DP6 combined with synthetic ice modulators in presence and absence of biological tissues

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