Effect of cryopreservation on zona - binding capacity of canine spermatozoa in vitro

Ivanova, Mariia; Mollova, M.; Kicheva, M.G. Ivanova; Petrov, M.; Djarkova, Ts.; Somlev, B

doi:10.1016/s0093-691x(99)00118-1

Cited by 24 publications

(15 citation statements)

References 10 publications

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“…Studies in other species have indicated a correlation between pregnancy success via AI and semen quality assessed by in vitro tests [28][29][30]. Likewise, the findings in the present study indicate a correlation between the results from in vitro tests of semen quality and pregnancy success.…”

Section: Discussionsupporting

confidence: 83%

The Efficacy of In Vitro Sperm Tests in Predicting Pregnancy Success after Artificial Insemination in the Bitch

Icn¹,

A²,

Cr³

et al. 2017

Andrology

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In many species, including the dog (Canis familiaris), semen analysis protocols has improved in recent years. In vitro assessment of semen quality provides important information; however, the ultimate parameter for determining semen quality is pregnancy outcome. The objective of the present study was to investigate the efficiency of in vitro sperm tests in predicting seminal quality in dogs by correlating those results with pregnancy success after artificial insemination (AI). Forty-eight semen samples refrigerated for 24, 48 or 72 hours were examined for sperm motility and progressive motility, membrane integrity, and zona pellucida-binding capacity before being used to artificially inseminate bitches. Results indicated positive correlations between pregnancy success and sperm motility (0.77), vigor (0.72), membrane integrity (0.72) and zona pellucida-binding (0.81). Both pregnancy success and the sperm characteristics declined with increased semen storage time. In conclusion, the semen characteristics evaluated in this study can be used to predict the conception potential of semen samples in the dog.

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Section: Discussionsupporting

confidence: 83%

The Efficacy of In Vitro Sperm Tests in Predicting Pregnancy Success after Artificial Insemination in the Bitch

Icn¹,

A²,

Cr³

et al. 2017

Andrology

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“…In the present study, oocytes were used that had an intact ZP after being cryopreserved in liquid nitrogen. Frozen/thawed samples were used because we wished to evaluate the ability of sperm to bind to the ZP [21][22][23][24]. In addition, Strom Holst et al [25] reported that deep freezing of ZP sources appears to be a better storage method than storing oocytes in salt solution, as is done in many studies.…”

Section: Discussionmentioning

confidence: 99%

Sperm Motility, Plasma Membrane Integrity, and Binding Capacity to Homologous Zona Pellucida of Cryopreserved Epididymal Spermatozoa in the Domestic Cat

Kashiwazaki

Yamaguchi

Uesugi³

et al. 2005

Journal of Reproduction and Development

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Abstract. We examined motility, plasma membrane integrity, and binding capacity to homologous zona pellucidae (ZP) of frozen/thawed epididymal cat sperm as a model species for endangered felines. Epididymal spermatozoa from 20 domestic cats were frozen with freezing egg-yolk extender containing 3.0% glycerol in 0.25-ml straws. Post-thaw motility and plasma membrane integrity of the frozen/thawed spermatozoa were 31.8 ± 2.4% and 32.2 ± 4.2%, respectively. The frozen/thawed spermatozoa were co-cultured with frozen/thawed immature homologous oocytes with intact ZP for 3 h to examine their ability to bind to the ZP. Sixteen of the 20 frozen/thawed sperm samples demonstrated the ability to bind to ZP. These results indicated that the freezing system for epididymal sperm used in the present study gives appropriate information for banking the genetic resources of wild felid species. Key words: Cat, Epididymal sperm, Freezing, Zona pellucida binding (J. Reprod. Dev. 51: [735][736][737][738][739] 2005) xcluding the domestic cat, all of the felidae f a m i l y a r e c o n s i d e r e d t h r e a t e n e d o r endangered, including Iriomote wild cats (Felis iriomotensis) and Tsushima leopard cats (Felis bengalensis euptilura), which are both mammals native to Japan [1]. Effective conservation and maintenance of an adequate diversity of animals can be achieved by preserving gametes and/or embryos as genetic resources in order to prevent the extinction of species [2]. Cryopreservation of epididymal spermatozoa offers a potential tool for rescuing genetic material from males that die unexpectedly, thus preserving gametes that could be used to maintain genetic variety within depleted

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“…Bisected by micromanipulation two parts of ZP are coincubated with spermatozoa. As a result the direct comparison of sperm cells from two origins may be done (Ivanova et al, 1999;MayencoAguirre & Pérez Cortés, 1998).…”

Section: Zona Pellucida Binding Assaymentioning

confidence: 99%

Methods of Assessment of Cryopreserved Semen

Partyka¹,

Niżański²,

Ochota³

2012

Current Frontiers in Cryobiology

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Effect of cryopreservation on zona - binding capacity of canine spermatozoa in vitro

Cited by 24 publications

References 10 publications

The Efficacy of In Vitro Sperm Tests in Predicting Pregnancy Success after Artificial Insemination in the Bitch

The Efficacy of In Vitro Sperm Tests in Predicting Pregnancy Success after Artificial Insemination in the Bitch

Sperm Motility, Plasma Membrane Integrity, and Binding Capacity to Homologous Zona Pellucida of Cryopreserved Epididymal Spermatozoa in the Domestic Cat

Methods of Assessment of Cryopreserved Semen

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