Effect of D-ribose-L-cysteine on aluminum induced testicular damage in male Sprague-Dawley rats

Abstract

ObjectiveThis study investigated the effects of D-ribose and L-cysteine on aluminum-induced testicular damage in male Sprague-Dawley rats.MethodA total number of thirty-five (35) adult male Sprague-Dawley rats were divided into four groups (AD). Group A (comprised five (5) rats) was designated the Control Group that received Physiological Saline; while groups B, C, and D (comprised ten (10) rats) were given 75 mg/kg, 150 mg/kg and 300 mg/kg of body weight of aluminum chloride respectively for 39 days. At day 40, the aluminum-treated groups were subdivided into sub-groups (B1, C1, D1) comprising of five (5) rats each, and 30 mg/kg body weight of Riboceine were administered for twenty (20) days. Groups B, C and D remained on the normal dosage of aluminum chloride for three more weeks (59 days).ResultsAndrological parameters (Sperm count, motility, morphology and testosterone) in the aluminum-treated Groups B and C showed no significant difference in their mean values when compared with their control counterparts, whereas there was a significant reduction in the andrological parameters in Group D rats when compared with the Control animals. Histoarchitecture of the testes "stain with H&E" of Group A, B and C rats appeared normal while Group D rats showed testicular damages with several abnormal seminiferous tubules with incomplete maturation of germinal cell layers and absence of spermatozoa in their lumen; Leydig cells appear hyperplastic. Group B1, C1 and D1 andrological and histological parameters appeared normal.ConclusionRiboceine treatment significantly attenuates aluminum-induced testicular toxicity in male Sprague-Dawley in rats.

Hormonal changes and redox imbalance in nicotine-induced testicular toxicity: the mitigating influence of D-ribose l-cysteine

“…It also potentiated testicular enzymatic anti-oxidant levels and induced a reduction in lipid peroxidation end-product (MDA). These observations also included increase in total tissue protein and they agree with the work of Falana et al (2017) which reported that the administration of Dribose L-cysteine was found to boost fertility in male Wistar rat. The mechanism of action of the of D-ribose L-cysteine lies in the interaction between its D-ribose and L-cysteine components that facilitates the production of glutathione.…”

Protective effects of vitamin E on aluminium sulphate-induced testicular damage

“…In another study, Al chloride had no effects on any of the andrological parameters (i.e. sperm count, motility, morphology and serum testosterone) (Falana et al, 2017). Decreases in these andrological parameters were only seen for high-dose Al chloride (Falana et al, 2017;Mouro et al, 2018).…”

“…Previous studies have shown that in seminiferous tubules following Al treatment, the germinal epithelium was thinner than normal, there were almost no spermatids present, the sperm count was low, and there were no sperms in the lumen. While intercellular bonds were seen to be broken, some free cells were detected in the lumens of the seminiferous tubules (Falana et al, 2017;Kutlubay et al, 2007). In another study, no histological changes were seen for the testicular structure of rats exposed to Al, in terms of the Sertoli cells and the germ cells at different developmental stages and those in the lumen (Mouro et al, 2018).…”

“…sperm count, motility, morphology and serum testosterone) (Falana et al, 2017). Decreases in these andrological parameters were only seen for high-dose Al chloride (Falana et al, 2017;Mouro et al, 2018). In rats treated with Al sulphate, the sperm count and morphologically abnormal sperm percentages in the cauda epididymis have been reported not to change (Hirata-Koizumi et al, 2011).…”

A comparison of the anti-diabetic potential of d-ribose-l-cysteine with insulin, and oral hypoglycaemic agents on pregnant rats

“…This confirms some level of dependence on non-enzymatic enzymes in the prevention of oxidative stress [ 25 , 47 , 48 ]. In our previous study, Riboceine attenuated aluminium –induced testicular damage [ 49 ]. This corroborates with the findings of this study, in addition, all the OHAs used in this study exemption of glibenclamide had similar effects with insulin in impeding lipid peroxidation.…”