ObjectiveThis study investigated the effects of D-ribose and L-cysteine on aluminum-induced testicular damage in male Sprague-Dawley rats.MethodA total number of thirty-five (35) adult male Sprague-Dawley rats were divided into four groups (AD). Group A (comprised five (5) rats) was designated the Control Group that received Physiological Saline; while groups B, C, and D (comprised ten (10) rats) were given 75 mg/kg, 150 mg/kg and 300 mg/kg of body weight of aluminum chloride respectively for 39 days. At day 40, the aluminum-treated groups were subdivided into sub-groups (B1, C1, D1) comprising of five (5) rats each, and 30 mg/kg body weight of Riboceine were administered for twenty (20) days. Groups B, C and D remained on the normal dosage of aluminum chloride for three more weeks (59 days).ResultsAndrological parameters (Sperm count, motility, morphology and testosterone) in the aluminum-treated Groups B and C showed no significant difference in their mean values when compared with their control counterparts, whereas there was a significant reduction in the andrological parameters in Group D rats when compared with the Control animals. Histoarchitecture of the testes "stain with H&E" of Group A, B and C rats appeared normal while Group D rats showed testicular damages with several abnormal seminiferous tubules with incomplete maturation of germinal cell layers and absence of spermatozoa in their lumen; Leydig cells appear hyperplastic. Group B1, C1 and D1 andrological and histological parameters appeared normal.ConclusionRiboceine treatment significantly attenuates aluminum-induced testicular toxicity in male Sprague-Dawley in rats.

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BackgroundThe aim of this study was to examine whether neem Azadirachta indica possesses regenerative potential on the stomach and ileum at 500 mg/kg dose given every 12 hours after mucosa lesion was brought by the administration of 1 ml of 50% ethanol for 21 consecutive days in adult Wistar rats.MethodsAdult male Wistar rats used in the study were divided into 3 groups: group A received oral normal saline and served as control; group B received 1.0 ml of 50% ethanol orally every 12 hours; and group C received neem extract (500 mg/kg) orally 12 hours after ethanol (50%, 1.0 ml) administration to verify its regenerative potential. The experiment lasted for 21 days after which the animals were sacrificed following chloroform inhalation and the stomach and ileum excised and processed for histological and morphometric examinations.ResultsEthanol treated rats showed marked gross mucosal lesions in the stomach and ileum. Ulcerated mucosa with marked apoptotic bodies and destruction of glandular elements were evident in the animals (group B). Neem extract administered 12 hours after the ethanol administration showed regenerative potential against ethanol-induced mucosal damage. This was characterized by mild restoration of the ulcerated mucosa epithelium and reorganization of the cyto-architechtural outline in group C.ConclusionsOur investigation suggests that neem extract has a regenerative potential and may be adopted in the management of gastrointestinal disorders such as ulcer.

Cyanide is one of the toxic, hazardous metals widely dispersed in the environment at high levels. The aim of this study is to evaluate the ameliorative role of Naringenin on male reproductive parameters in cyanide exposed mice.A total number of 28 Albino mice were divided into four groups, each group comprises of 7 mice (n= 7). The animals were housed in a well-lighted and ventilated plastic cages at a controlled temperature with 12h light/dark cycle maintained throughout the experimental period. All the Mice were acclimatized for 2 weeks before commencement of the study. Group 1 were control mice, group 2 received cyanide (1.2mg/kg bw) only, group 3 received Cyanide (1.2mg/kg bw) and Naringenin (50mg/kg bw) daily and group 4 received a daily administration of Naringenin (50mg/kg bw). All the treatments were done at 7:00 am every morning and the experiment lasted for 14 days. Twenty-four hours after 14th day of treatment, animals were sacrificed by cervical dislocation. Blood samples were collected via Ocular sinus into lithium-heparin bottles for haematological and hormonal assay. The right testis was excised and quickly placed in Bouin's fluid and processed for histological examination while the left testis was placed in sucrose and processed for antioxidant assay.Results from this study showed significant reduction in serum testosterone levels, oxidative damage, reduced packed cell volume (PVC), reduced body weight gain and degenerative testicular microarchitecture in mice exposed to cyanide compared to control. Administration of Naringenin  reversed almost all the abnormalities in the parameters investigated showing significant protection against cyanide induced toxicity in mice. It is concluded that Naringenin showed affordable protection against cyanide induced toxicity on male reproductive profile. Keywords: Naringenin, cyanide, oxidative damage, testis.

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