Effect of Deacetylation Degree of Chitosan as Solid Support in Lipase Immobilization by Glutaraldehyde Crosslink

Modifying Thermostability and Reusability of Hyperthermophilic Mannanase by Immobilization on Glutaraldehyde Cross-Linked Chitosan Beads

“…The mixture was boiled for 10 min and the absorbance was measured at 405 nm after cooling the reaction mixture on ice (0 °C). One unit of enzyme is the amount that produced 1 µmol of mannose sugar per minute under the said assay conditions [ 21 ].…”

“…The covalent immobilization of enzyme on chitosan occurs by treating it will glutaraldehyde crosslinker. The bifunctional –CHO groups of glutaraldehyde react with the –NH2 groups present in chitosan and in the enzyme [ 21 ]. Işık et al [ 22 ] used glutaraldehyde cross-linked chitosan beads for covalent immobilization of acetylcholinesterase (AChE) enzyme which resulted in high stability and reusability of enzyme.…”

Production and characterization of chitooligosaccharides by the fungal chitinase Chit42 immobilized on magnetic nanoparticles and chitosan beads: selectivity, specificity and improved operational utility

“…Therefore, some chitosanases, lipases, amylases and laccases (among others) have been already immobilized using MNPs and/or CMS with successful results. 11,[14][15][16][17][18] In contrast, most chitinases previously immobilized using both types of supports lost their activity aer being reused in consecutive cycles or no data concerning production and characterization of COS were reported. [19][20][21] Chitinase Chit42 from the fungus Trichoderma harzianum was previously expressed in the yeast Pichia pastoris with about 3 g L À1 of the protein produced in this heterologous system.…”