Effect of delayed supplementation of fetal calf serum to culture medium on bovine embryo development in vitro and following transfer

Thompson, Jeremy G.; Allen, Nickolas; McGowan, Lindsay T.; Bell, A.C.S.; Lambert, M. G.; Tervit, H.R.

doi:10.1016/s0093-691x(98)00071-5

Cited by 113 publications

(54 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the present work, the addition of serum promoted the earlier appearance of Ž blastocysts, which has been reported by other authors Bavister et al, 1992;Bavister, 1995;Carolan et al, 1995;Koji et al, 1997;Thompson . et al, 1998a;Tricoire et al, 1999 .…”

Section: Discussionsupporting

confidence: 91%

“…al., 1997;Wang et al, 1997;Donnay et al, 1999 and absence of exogenous glucose Ž . Vansteenbrugge et al, 1997;Thompson et al, 1998a . The FCS used in these experiments added 0.54 mM glucose to culture media, whilst Ž glucose contents were substantially lower in BSA and UBOCM below 0.12 and 0.05 .…”

Section: Discussionmentioning

confidence: 99%

“…et al, 1998a;Tricoire et al, 1999 . The effect of FCS accelerating blastocyst develop-Ž ment became apparent even after brief periods of culture Koji et al, 1997;Thompson et al, 1998a; these works reported differences in blastocyst rates because of serum addition at 36 h, 46 h and 2 days of culture, . respectively .…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of glucose and protein sources on bovine embryo development in vitro

Gómez

Díez

2000

Animal Reproduction Science

View full text Add to dashboard Cite

Oviductal factors may be obtained by ultrafiltration of conditioned medium, added to a simple media and used in bovine embryo culture. In this study, we aimed to analyze the development of bovine embryos produced with oviductal factors compared to those cultured in the presence of BSA or serum, the effects of glucose in presence of these protein supplements, and the ability of oviductal factors to support embryo development during the entire culture period. In vitro produced bovine zygotes from slaughterhouse ovaries were cultured in modified-synthetic oviduct Ž . Ž . Ž . Ž . fluid mSOF alone or supplemented with 1 oviductal factors, 2 BSA and 3 FCS. Oviductal factors showed embryotrophic activity, although with blastocyst rates lower than those in BSA and Ž . FCS. Glucose 1.5 mM added at Day 2 of culture did not affect development in the presence of oviductal factors. The number of cells in expanded blastocysts was unaffected by the presence of glucose or any of the protein supplements used. Both BSA and FCS, respectively, improved blastocyst rates of Day 6 embryos produced with oviductal factors. The effect of oviductal factors was masked by the presence of BSA during the entire culture. FCS promoted an earlier appearance of blastocysts. It is concluded that the effect of glucose on in vitro embryo development depends upon the source of protein. Oviductal factors are not an appropriate supplement for embryos beyond Day 6 of culture in SOF, although blastocyst rates of such embryos may be increased by culturing them in the presence of FCS or BSA. q

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of glucose and protein sources on bovine embryo development in vitro

Gómez

Díez

2000

Animal Reproduction Science

View full text Add to dashboard Cite

show abstract

“…However, within embryos produced with sexed spermatozoa, such an increase was nonsignificant. It is known that the addition of serum to culture media has a biphasic effect on embryo development, inhibiting the early stages and promoting the development of morulae and blastocysts [57,58]. On the other hand, the use of Vero cells shows an embryotrophic effect by producing growth factors and also removing toxicants or inhibitory substances derived from the embryo [59].…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

et al. 2012

View full text Add to dashboard Cite

In this work we analyzed the effects of three culture systems on developmental ability of bovine embryos in vitro produced with sexed sperm, the survival to vitrification (cryologic vitrification method) of such blastocysts, and their pregnancy rates after embryo transfer to recipients, both as fresh and after vitrification/warming. Finally, we measured the accuracy of the sorting protocol by a polymerase chain reaction-based method to validate the embryo sex at blastocyst stages. We confirmed an individual effect of the bull as well as development rates of embryos produced with sorted sperm lower than embryos with unsorted sperm, independent of the culture system used. The cryoresistance to vitrification of embryos produced with sexed sperm did not differ from that of conventionally produced embryos (re-expansion rates at 24 and 48 h: 74.6% vs. 75.5%, and 64.5% vs. 68.1% for embryos produced with conventional and sorted sperm, respectively; hatching rates at 48 h: 63.55% vs. 55.5% for embryos produced with conventional and sorted sperm, respectively). Finally, no significant differences were found in pregnancy rates after the embryo transfer of fresh and vitrified/warmed blastocysts (52.8% vs. 42.0%, respectively; P Ͼ 0.05). Male and female embryos produced with sorted sperm showed the same quality in terms of developmental ability, cryoresistance, and pregnancy rates after transfer. Our culture system, coupled with the vitrification in fiber plugs, provides good quality sex-known embryos which survive vitrification at similar rates than embryos produced with conventional unsorted sperm; also it produces good pregnancy rates after transfer of sexed embryos both fresh and after vitrification and warming.

show abstract

“…The structure of the zona pellucida differs between IVP and in vivo embryos with respect to the exposure and coating of the zona pellucida to oviductal glycoproteins (e.g. [150]) and its resistance to proteases (lower in IVP embryos) [107] Pregnancy loss following transfer of IVP embryos is not only higher in early gestation prior to day 23, but abortion rates after 2 months of gestation is also higher (7-24 %) [54,78,110,111,135,142] [78] reported that the percentage of calves weighing more than 50 and 60 kg, respectively, were < 10 % versus 2 32 % and < 4 % versus 2 6 % for in vivo compared to IVP calves. In the two latter studies, the incidence of perinatal death was also significantly higher for IVP calves (9-14 %) compared to in vivo calves (6 %).…”

mentioning

confidence: 99%