Effect of DGAT1 variant (K232A) on milk traits and milk fat composition in outdoor pasture-grazed dairy cattle

Li, Yunhai; Zhou, Huitong; Cheng, Long; Edwards, Grant; Hickford, Jonathan G. H.

doi:10.1080/00288233.2019.1589537

Cited by 16 publications

(19 citation statements)

References 28 publications

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“…Both DGAT1 p.K232A and the variants of FABP4 described here affected the MCFA and LCFA levels, but their effects on the individual FA levels were different, both in terms of the specific FA level affected and the size of the effect. For example, Li et al (2019) reported that DGAT1 p.K232A only appeared to affect the C11:0, C13:0, C14:0, C16:0 and C17:0 levels. This suggests the effect of FABP4 variation on C10:0, C12:0, C20:0, C22:0 and C24:0 FA levels described here, are independent effects.…”

Section: Resultsmentioning

confidence: 99%

“…For example, Li et al . (2019) reported that DGAT1 p.K232A only appeared to affect the C11:0, C13:0, C14:0, C16:0 and C17:0 levels. This suggests the effect of FABP4 variation on C10:0, C12:0, C20:0, C22:0 and C24:0 FA levels described here, are independent effects.…”

Section: Resultsmentioning

confidence: 99%

“…Using the same blood samples, DGAT1 p.K232A was investigated. The genotyping method for DGAT1 p.K232A was as described by Li et al (2019).…”

Section: Pcr-sscp Analysis and Genotypingmentioning

confidence: 99%

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Effects of FABP4 variation on milk fatty-acid composition for dairy cattle grazed on pasture in late lactation

Zhou

Cheng

et al. 2020

Journal of Dairy Research

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This research communication describes associations between variation in the fatty acid binding protein 4 gene (FABP4) and milk fat composition in New Zealand Holstein-Friesian × Jersey cross dairy cows. After correcting for the effect of the amino acid substitution p.K232A in diacylglycerol O-acyltransferase 1 (DGAT1), which is associated with variation in many milk fatty acid (FA) component levels, the effect of FABP4 c.328A/G on milk FA levels was typically small. For the five genotypes analysed, the AB cows produced more medium-chain fatty acids than CC cows (P < 0.05), and more C14:0 FA than AA and AC cows (P < 0.05). The AA and AC cows produced less C22:0 FA (P < 0.01) than the BC cows, and the AC cows produced more C24:0 FA (P < 0.05) than was produced by the BC cows. Cows of genotype CC produce more long-chain fatty acids than cows of genotype BC (P < 0.05).

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Effects of FABP4 variation on milk fatty-acid composition for dairy cattle grazed on pasture in late lactation

Zhou

Cheng

et al. 2020

Journal of Dairy Research

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“…However, this may also have some negative effects. Some literature indicates that the variants led to increased milk components, but reduced the milk yield [46][47][48]. Regrettably, in this study, because of the lack of data on the milk yield of dairy goats, the effect of this indel on this aspect cannot be studied.…”

Section: Discussionmentioning

confidence: 86%

An 11-bp Indel Polymorphism within the CSN1S1 Gene Is Associated with Milk Performance and Body Measurement Traits in Chinese Goats

Zhang

Wang

Liu

et al. 2019

Animals

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Simple Summary: In the field of animal breeding, selection using molecular genetic markers, such as insertions/deletions (indels), is a novel method to reduce the generation interval and more accurately improve performance. The casein alpha s1 (CSN1S1) gene is one of the major genes regulating the milk performance of mammals, and it is also relevant to body development. Previous studies have shown that an 11-bp indel in this gene was strongly associated with goat litter size, however, its effects on milk performance and body measurement traits have not been studied. In this study, this indel was genotyped in three Chinese goat breeds, and further analysis results revealed that this indel polymorphism was significantly associated with milk performance in dairy goats, as well as affecting the body measurement traits in all three breeds. These findings hint that the 11-bp indel within CSN1S1 gene could be utilized for effectively selecting goats with excellent performances in terms of production and breeding.Abstract: The casein alpha s1 (CSN1S1) gene encodes α-s1 casein, one of the proteins constituting milk, which affects milk performance, as well as improving the absorption of calcium and bone development in mammals. A previous study found that an 11-bp insertion/deletion (indel) of this gene strongly affected litter size in goats. However, to our knowledge, the relationships between this polymorphism and the milk performance and body measurement traits of goats have not been reported. In this paper, the previously identified indel has been recognized in three Chinese goat breeds, namely the Guanzhong dairy goat (GZDG; n = 235), Shaanbei white cashmere goat (SBWC; n = 1092), and Hainan black goat (HNBG; n = 278), and the following three genotypes have been studied for all of the breeds: insertion/insertion (II), deletion/deletion (DD), and insertion/deletion (ID). The allele frequencies analyzed signified that the frequencies of the "D" allele were higher (47.8%-65.5%), similar to the previous report, which indicates that this polymorphism is genetically stable in different goat breeds. Further analysis showed that this indel was markedly associated with milk fat content, total solids content, solids-not-fat content, freezing point depression, and acidity in GZDG (p < 0.05), and also affected different body measurement traits in all three breeds (p < 0.05). The goats with II genotypes had superior milk performance, compared with the others; however, goats with DD genotypes had better body measurement sizes. Hence, it may be necessary to select goats with an II or DD genotype, based on the desired traits, while breeding. Our study Animals 2019, 9, 1114 2 of 11 provides information on the potential impact of the 11-bp indel polymorphism of the CSN1S1 gene for improving the milk performance and body measurement traits in goats.

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“…Unsaturated FA indices were also calculated as follows: C12:1 index (C12:1 divided by the sum of C12:0 and C12:1); C16:1 index (C16:1 cis-9 divided by the sum of C16:0 and C16:1 cis-9); and C18:1 index (C18:1 cis-9 divided by the sum of C18:0 and C18:1 cis-9). The method is as described by Li et al [17], with the un-adjusted mean levels in the 430 cows being calculated and used subsequently in the statistical analyses.…”

Section: Gas Chromatography Of the Fatty Acids In The Milk Samplesmentioning

confidence: 99%

Associations between the Bovine Myostatin Gene and Milk Fatty Acid Composition in New Zealand Holstein-Friesian × Jersey-Cross Cows

Haruna

Ekegbu

et al. 2020

Animals

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The myostatin gene (MSTN), which encodes the protein myostatin, is pleiotropic, and its expression has been associated with both increased and decreased adipogenesis and increased skeletal muscle mass in animals. In this study, the polymerase chain reaction, coupled with single strand conformation polymorphism analysis, was utilized to reveal nucleotide sequence variation in bovine MSTN in 410 New Zealand (NZ) Holstein-Friesian × Jersey (HF × J)-cross cows. These cows ranged from 3 to 9 years of age and over the time studied, produced an average 22.53 ± 2.18 L of milk per day, with an average milk fat content of 4.94 ± 0.17% and average milk protein content of 4.03 ± 0.10%. Analysis of a 406-bp amplicon from the intron 1 region, revealed five nucleotide sequence variants (A–E) that contained seven nucleotide substitutions. Using general linear mixed-effect model analyses the AD genotype was associated with reduced C10:0, C12:0, and C12:1 levels when compared to levels in cows with the AA genotype. These associations in NZ HF × J cross cows are novel, and they suggest that this variation in bovine MSTN could be explored for increasing the amount of milk unsaturated fatty acid and decreasing the amount of saturated fatty acid.

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Effect of DGAT1 variant (K232A) on milk traits and milk fat composition in outdoor pasture-grazed dairy cattle

Cited by 16 publications

References 28 publications

Effects of FABP4 variation on milk fatty-acid composition for dairy cattle grazed on pasture in late lactation

Effects of FABP4 variation on milk fatty-acid composition for dairy cattle grazed on pasture in late lactation

An 11-bp Indel Polymorphism within the CSN1S1 Gene Is Associated with Milk Performance and Body Measurement Traits in Chinese Goats

Associations between the Bovine Myostatin Gene and Milk Fatty Acid Composition in New Zealand Holstein-Friesian × Jersey-Cross Cows

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