Effect of Dietary Flaxseed Oil Supplementation on the Redox Status, Haematological and Biochemical Parameters of Horses’ Blood

Abstract

This study compared the effect of two dietary vegetable oils on plasma biochemical indices, haematological parameters, and redox status of horses. Forty riding horses (20 mares and 20 stallions) of the Malopolski breed were divided equally into two groups that were similar in terms of age, sex, and body weight (on average 530 ± 30 kg). The horses received soybean oil (SO) or flaxseed oil (FO) in the amount of 25 mL per 100 kg BW/day. After 60 days, blood was collected for biochemical and haematological analyses. The results show that horses receiving FO as compared to the SO group had significantly lower plasma levels of glucose, low density lipoprotein cholesterol, total cholesterol/high-density lipoprotein (HDL) ratio and triacylglycerols, as well as the activities of alanine aminotransferase and alkaline phosphatase. In turn, %HDL-TC and lactate dehydrogenase activity were significantly higher in the FO group. The inclusion of FO in the diet contributed to an increase in antioxidant indices: creatinine, vitamin C, copper, and zinc contents and also superoxide dismutase and catalase activities. The level of the end product of lipid peroxidation, i.e., malonyl dialdehyde, in the FO group as compared to the SO group was significantly lower. Moreover, FO caused an elevation in red blood cell indicators, lymphocyte count and lysozymes. In conclusion, FO exerts a beneficial effect by stimulating antioxidant defence mechanisms of horses and reducing the severity of oxidative stress. FO also improved the lipid profile and haematological parameters of the blood. The replacement of SO by FO is recommended based on these findings.

Study on Toxic Effects of Lead Acetate on Haematological Parameters of Adult Albino Rats and The Role of Mesenchymal Stem Cells and Flax Seeds Oil as Therapeutic Agents

“…Our results showed that rats treated with flax seeds oil improved in total leucocytes, lymphocytes, neutrophils, eosinophils and monocytes. Hendawi et al, 2016 reported that rats treated with flax seeds oil amelioration total white cells and their differentiation, this improvement may be attributed to flax seeds oil's protective effect through the high content of antioxidant agents such as beta-carotene and tocopherols.Flax seeds oil enhanced leucogram in horses, flax seeds oil exerts a beneficial effect by stimulating antioxidant defense mechanisms of horses and reducing the severity of oxidative stress (Sembratowicz et al, 2020). Basophils in all groups of this study exhibited zero.…”

“…Flaxseed oil improved hematological parameters of the blood (RBCs, Hb, PCV and blood indices) in horses, flaxseed oil exerts a beneficial effect by stimulating antioxidant defense mechanisms of horses and reducing the severity of oxidative stress (Sembratowicz et al, 2020).…”

“…Lignans present in flaxseeds also exhibit antioxidant properties and additionally function as phytoestrogens (Pilar et al, 2017). The beneficial effect of flaxseed oil on the alleviation of oxidative stress accompanying various disease entities or intoxication and positively influence blood biomarkers (Sembratowicz et al, 2020).…”

Particle Size Distribution of Plasma Lipoproteins in Donkeys from Death Valley Compared to a Sampling of Horses

“…Thus, reagents developed for the quantification of human HDL and LDL are frequently proven inaccurate in veterinary species [ 11 , 12 ]. Several studies evaluating HDL and LDL in equids have not included or cited validation of the method for use in equids [ 13 , 14 ]. The Lipoprint ® assay uses high-resolution polyacrylamide gel electrophoresis to rapidly assess human LDL and HDL at the subfraction level.…”

Effects of Crude Rice Bran Oil and a Flaxseed Oil Blend in Young Horses Engaged in a Training Program

“…Oils such as rice bran and flaxseed are marketed as multipurpose equine nutritional supplements. Both oils contain components that reduce oxidative damage and inflammation, including phytosterols and vitamin E, which contain tocopherols and tocotrienol [ 11 ]. Both oils contain essential fatty acids; however, flaxseed oil contains a greater amount of the anti-inflammatory omega-3 fatty acid, alpha-linolenic acid (ALA), than rice bran oil.…”