Effect of Dietary Rumen-Protected L-Tryptophan Supplementation on Growth Performance, Blood Hematological and Biochemical Profiles, and Gene Expression in Korean Native Steers under Cold Environment

Lee, Jae-Sung; Priatno, Wahyu; Lee, Hong Gu; Peng, Dong Qiao; Park, Jin-Seung; Moon, Jungyoon; Lee, Hong-Gu

doi:10.3390/ani9121036

Cited by 12 publications

(23 citation statements)

References 28 publications

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“…It should be noted that we collected blood samples prior to feeding, and thus due its half-life (1.8 h in in-vitro culture [23]) and also body homeostasis mechanism, rumen protected TRP may not alter blood metabolite parameters including TP, ALB, GLO, BUN, CPK, GLU, NEFA, and TG. Consistent with our results, in the previous study under cold stress conditions, Lee et al [3] reported unchanged blood biochemical parameters by supplementation of 0.1% rumen-protected L-TRP in Hanwoo steers, when blood samples were collected prior to feeding. Further study with a larger sample size and with the addition of a higher amount of TRP supplementation may result in significant differences in blood biochemical values particularly when blood was collected after feeding.…”

Section: Resultssupporting

confidence: 93%

“…TRP is an important factor for erythropoiesis which produces red blood cells, which is the development of erythropoietic stem cells to mature red blood cells [19]. Similar with these complete blood count (CBC) results, Lee et al [3] found no changes in WBC, LYM, GRA, RBC, HGB, HCT, mean corpuscular hemoglobin (MCH), and PLT of steers fed with 0.1% rumen-protected TRP. All blood biochemical parameters including ALB, GLO, GLU, CPK, NEFA, BUN, TG, and TP were not changed by supplementation of TRP (p > 0.05; Table 4).…”

Section: Resultsmentioning

confidence: 74%

“…The amount added was triple times higher than the concentration provided in the control diet (Table 1). The rate of by-pass was indicated above 95% [3]. TRP was included at 0.829 g per 1 g of rumen-protected TRP.…”

Section: Animals and Managementmentioning

confidence: 99%

“…The following PCR conditions were used: 95℃ for 3 min and 40 cycles at 95℃ for 10 s, 51℃ to 61℃ for 30 s and 72℃ for 30 s. 18S ribosomal RNA (18S), glyceraldehyde3-phosphate dehydrogenase (GAPDH), and ribosomal protein lateral stalk subunit P0 (RPLP0) primers were designed using the National Center for Biotechnology Information website. 18S, GAPDH, and RPLP0 were used as housekeeping genes [3,14] ( Table 2).…”

Section: Synthesis Of Cdna and Gene Expression Analysismentioning

confidence: 99%

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“Dietary supplementation of L-tryptophan” increases muscle development, adipose tissue catabolism and fatty acid transportation in the muscles of Hanwoo steers

Priatno¹,

Jo²,

Lee³

et al. 2020

J Anim Sci Technol

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This study investigated the effects of dietary rumen-protected L-tryptophan (TRP) supplementation (43.4 mg of L-tryptophan kg −1 body weigt [BW]) for 65 days in Hanwoo steers on muscle development related to gene expressions and adipose tissue catabolism and fatty acid transportation in longissimus dorsi muscles. Eight Hanwoo steers (initial BW = 424.6 kg [SD 42.3]; 477 days old [SD 4.8]) were randomly allocated to two groups (n = 4) of control and treatment and were supplied with total mixed ration (TMR). The treatment group was fed with 15 g of rumen-protected TRP (0.1% of TMR as-fed basis equal to 43.4 mg of TRP kg −1 BW) once a day at 0800 h as top-dressed to TMR. Blood samples were collected 3 times, at 0, 5, and 10 weeks of the experiment, for assessment of hematological and biochemical parameters. For gene study, the longissimus dorsi muscle samples (12 to 13 ribs, approximately 2 g) were collected from each individual by biopsy at end of the study (10 weeks). Growth performance parameters including final BW, average daily gain, and gain to feed ratio, were not different (p > 0.05) between the two groups. Hematological parameters including granulocyte, lymphocyte, monocyte, platelet, red blood cell, hematocrit, and white blood cell showed no difference (p > 0.05) between the two groups except for hemoglobin (p = 0.025), which was higher in the treatment than in the control group. Serum biochemical parameters including total protein, albumin, globulin, blood urea nitrogen, creatinine phosphokinase, glucose, nonesterified fatty acids, and triglyceride also showed no differences between the two groups (p > 0.05). Gene expression related to muscle development (Myogenic factor 6 [MYF6], myogenine [MyoG]), adipose tissue catabolism (lipoprotein lipase [LPL]), and fatty acid transformation indicator (fatty acid binding protein 4 [FABP4]) were increased in the treatment group compared to the control group (p < 0.05). Collectively, supplementation of TRP (65 days in this study) promotes muscle development and increases the ability of the animals to catab

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 74%

Section: Animals and Managementmentioning

confidence: 99%

Section: Synthesis Of Cdna and Gene Expression Analysismentioning

confidence: 99%

See 2 more Smart Citations

“Dietary supplementation of L-tryptophan” increases muscle development, adipose tissue catabolism and fatty acid transportation in the muscles of Hanwoo steers

Priatno¹,

Jo²,

Lee³

et al. 2020

J Anim Sci Technol

Self Cite

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show abstract

“…Blood sampling for blood cell counting [ 11 ] and metabolite analysis [ 12 , 13 ] was conducted for each cow via the jugular vein at 3 h before feeding on the first day at 0, 4, and 8 weeks. In brief, blood was collected into an EDTA tube (BD 367844 Vacutainer, Becton Dickinson, NJ, USA) and then subjected to a CBC test using an HM2 (VetScan HM2 Hematology System, Abaxis, Union City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

In Vitro and In Vivo Studies of Rumen-Protected Microencapsulated Supplement Comprising Linseed Oil, Vitamin E, Rosemary Extract, and Hydrogenated Palm Oil on Rumen Fermentation, Physiological Profile, Milk Yield, and Milk Composition in Dairy Cows

Kim

Lee

Cho³

et al. 2020

Animals

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The aim of the present study was to evaluate the effects of adding dietary rumen-protected microencapsulated supplements into the ruminal fluid on the milk fat compositions of dairy cows. These supplements comprised linseed oil, vitamin E, rosemary extract, and hydrogenated palm oil (MO; Microtinic® Omega, Vetagro S.p.A, Reggio Emilia, Italy). For in vitro ruminal fermentation, Holstein–Friesian dairy cows each equipped with a rumen cannula were used to collect ruminal fluid. Different amounts (0%, 1%, 2%, 3%, 4%, and 5%) of MO were added to the diets to collect ruminal fluids. For the in vivo study, 36 Holstein–Friesian dairy cows grouped by milk yield (32.1 ± 6.05 kg/d/head), days in milk (124 ± 84 d), and parity (2 ± 1.35) were randomly and evenly assigned to 0.7% linseed oil (LO; as dry matter (DM) basis) and 2% MO (as DM basis) groups. These two groups were fed only a basal diet (total mixed ration (TMR), silage, and concentrate for 4 weeks) (period 1). They were then fed with the basal diet supplemented with oil (0.7 LO and 2% MO of DM) for 4 weeks (period 2). In the in vitro experiment, the total gas production was found to be numerically decreased in the group supplemented with 3% MO at 48 h post in vitro fermentation. A reduction of total gas production (at 48 h) and increase in ammonia concentration (24 h) were also observed in the group supplemented with 4% to 5% MO (p < 0.05). There were no differences in the in vitro fermentation results, including pH, volatile fatty acids, or CH4 among groups supplemented with 0%, 1%, and 2% MO. The results of the in vitro study suggest that 2% MO is an optimal dosage of MO supplementation in cows’ diets. In the in vivo experiment, the MO supplement more significantly (p < 0.01) increased the yield of total w3 fatty acids than LO (9.24 vs. 17.77 mg/100 g milk). As a result, the ratio of total omega-6 to omega-3 fatty acids was decreased (p < 0.001) in the MO group compared to that in the LO group (6.99 vs. 3.48). However, the milk yield and other milk compositions, except for milk urea nitrogen, were similar between the two groups (p > 0.05). Collectively, these results suggest that the dietary supplementation of 2% MO is beneficial for increasing omega-3 fatty acids without any negative effects on the milk yield of dairy cows.

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Dietary supplementation of acetate-conjugated tryptophan alters feed intake, milk yield and composition, blood profile, physiological variables, and heat shock protein gene expression in heat-stressed dairy cows

Lee

Moon²,

Lee

2021

Journal of Thermal Biology

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Effect of Dietary Rumen-Protected L-Tryptophan Supplementation on Growth Performance, Blood Hematological and Biochemical Profiles, and Gene Expression in Korean Native Steers under Cold Environment

Cited by 12 publications

References 28 publications

“Dietary supplementation of L-tryptophan” increases muscle development, adipose tissue catabolism and fatty acid transportation in the muscles of Hanwoo steers

“Dietary supplementation of L-tryptophan” increases muscle development, adipose tissue catabolism and fatty acid transportation in the muscles of Hanwoo steers

In Vitro and In Vivo Studies of Rumen-Protected Microencapsulated Supplement Comprising Linseed Oil, Vitamin E, Rosemary Extract, and Hydrogenated Palm Oil on Rumen Fermentation, Physiological Profile, Milk Yield, and Milk Composition in Dairy Cows

Dietary supplementation of acetate-conjugated tryptophan alters feed intake, milk yield and composition, blood profile, physiological variables, and heat shock protein gene expression in heat-stressed dairy cows

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