Effect of different concentrations of EDTA on smear removal and collagen exposure in periodontitis‐affected root surfaces

Abstract

The purpose of the present study was to explore the possibility of obtaining an acceptable smear-removing and collagen-exposing effect following EDTA etching with concentrations lower than supersaturation (24%). A flat dentin surface was created on human teeth extracted due to severe periodontitis. The teeth were etched with the following concentrations of EDTA for 2 min: 1.5% (group I), 5% (group II), 15% (group III) and 24% (group IV), and evaluated with scanning electron microscopy with respect to smear layer removal and exposure of root-associated collagen fibers. It was found that neither 1.5% nor 5% EDTA etching was sufficient to dissolve the smear layer to any higher degree. Etching with 15% EDTA dissolved the smear more effectively than the lower concentrations and collagen fibers were sparsely found. Following etching with 24% EDTA (group IV), significantly less smear was seen on the dentin surfaces than in any of the other groups and collagen fibers were seen covering the entire intertubular dentin surfaces. Based on these findings, the concentration of EDTA should be somewhere between 15 to 24% in order to obtain an acceptable smear removing and collagen-exposing effect within a clinically acceptable time period.

Immunohistochemical analysis of periodontal reattachment on denuded root dentin after periodontal surgery

“…(2) Immunohistochemistry Three serial sections (a total of 18 sections) were selected on both the cervical and apical sides of the middle, cervical middle, and apical middle sections in each defect. Nine sections of the cervical side planing accelerates the first step of periodontal reattachment, as surgically exposed collagen fibers are expected to intermingle with newly generated fibrous tissue (1,5,23). Further, recent clinical studies have reported that enamel matrix derivative (EMD) accelerates the regeneration of cementum, which is structurally similar to the original cementum (3,15).…”

Blood Cells Attachment after Root Conditioning and PRP Application: An in vitro Study

“…Samples in the groups II, III, IV and V were conditioned during 3 minutes and solutions were renewed every 30 seconds. [18][19][20] After the conditioning procedure samples were washed with 10 ml of saline solution.…”

Histological analysis of effects of 24% EDTA gel for nonsurgical treatment of periodontal tissues

“…Perhaps the most frequently attempted type of regeneration has involved chemical modification of the root surface (1). The purpose of this chemical modification is mainly to remove the smear layer that results from scaling and root planning (2,3), to remove endotoxins, to open dentinal tubules, as well as to expose collagen fibers from root surfaces (4)(5)(6)(7). Therefore chemical modification has been used as an adjunct to scaling and root planning to facilitate reattachment of periodontal ligament fibers (3,8).…”

“…One of the most widely used agents for this purpose is citric acid (9). However, other agents such as tetracycline (10)(11)(12) and ethylenediaminetetraacetic acid (EDTA) (6,13,14) have also been employed.…”