Effect of different cultivation conditions and inducers on the production of laccase by the litter-dwelling fungal isolate Fusarium incarnatum LD-3 under solid substrate fermentation

Application of Central Composite Designs Utilized for Scale-up Strategies in the Optimization Process for Development Laccase Production and for the Bioremediation of Various Industrial Pollutants

“…On the other hand, isopropanol caused a slight decline in laccase titer about 4.4% reduction was noticed, while a sharp reduction in laccase production was achieved by acetone, more than one-half of enzyme activity was lost. The present results are consistent with that obtained by other investigators who recorded that laccase production was increased in presence of ethanol and methanol, and suppressed in presence of acetone [16,19], but in The dye decolorization studies showed great variation in the ability of Alcaligenes faecalis NYSO (KP859538) to degrade dyes belonging to diverse categories. As well as laccase production varied greatly with a different dye-containing media ( Table 5).…”

“…where Y is the predicted response (laccase activity U ml-1 min-1); β 0 is the model intercept; X 1 , X 2 , and X 3 are the independent tained from culture control (1198 U ml -1 min -1 ). This find may be in accordance with others citation that reported the enhancement of laccase production in presence of L-glutamine, tyrosine, L-cystine, lysine, and isoleucine [16]. Contrary to other finding recorded that the L-valine, L-arginine, L-cysteine, and biotin caused repression of laccase titer [17,18] and phenylalanine, nicotinic acid and L-histidine HCl were recorded as enhancers for laccase production.…”

“…The cultures with ethanol expressed more laccase activity than the other treatments exhibiting an increase in activity and reaching a peak of 1679 U ml -1 min -1 in compared with culture control without any solvent (1198 U ml -1 min -1 ). The causes of increase laccase production in the presence of ethanol were previously explained by Chhaya., et al (2013) [16] as a result of its dual role, i.e. gene expression and inhibition of protease activity.…”

Fungal Laccase Production from Lignocellulosic Agricultural Wastes by Solid-State Fermentation: A Review

“…The genus Trametes includes some thermotolerant strains, but few of them have been studied at temperatures above 30 • C. Trametes trogii LK13 cultured at 37 • C showed enhancement of laccase activity, mycelial growth rate, thermostability, and tolerance to organic solvents [105]. A temperature increase from 40 to 50 • C was detrimental to the fungus Fusarium incarnatum and reduced its laccase production [106]. Higher temperatures may also adversely affect metabolic activities of fungi by denaturing key enzymes [4].…”

Optimization of different culture conditions for enhanced laccase production and its purification from Tricholoma giganteum AGHP

“…Xin and Geng (2011) have also reported that the lower temperature retards the metabolic rate of Trametes versicolor leading to the decrease in laccase production. Many researchers have reported an optimum temperature between 25 and 30 °C for laccase production using various white rot fungi (Ravikumar et al 2012;Chhaya and Gupte 2013;Elsayed et al 2012). …”