Effect of Different Endodontic Regeneration Protocols on Wettability, Roughness, and Chemical Composition of Surface Dentin

Yassen, Ghaeth H.; Sabrah, Alaa H.A.; Eckert, George J.; Platt, Jeffrey A.

doi:10.1016/j.joen.2015.02.023

Cited by 38 publications

(40 citation statements)

References 34 publications

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“…Furthermore, the Ca(OH) 2 regeneration protocol was found to cause significant reductions in dentine wettability in comparison with other regeneration protocols (Yassen et al . ). It is also worth noting that a previous clinical study found significantly less increases in dentinal wall thickness amongst endodontic regeneration cases treated with Ca(OH) 2 in comparison with cases treated with antibiotic medicaments (Bose et al .…”

Section: Discussionmentioning

confidence: 97%

Attachment and proliferation of dental pulp stem cells on dentine treated with different regenerative endodontic protocols

et al. 2016

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Section: Discussionmentioning

confidence: 97%

Attachment and proliferation of dental pulp stem cells on dentine treated with different regenerative endodontic protocols

et al. 2016

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“…This high concentration was suggested to have unfavorable effects on stem cells from apical papillae (14) and dental pulp stem cells (22), as well as on the chemical (23), physical (19), and mechanical properties of dentin (24). Therefore, the current study investigated the ability of a creamy consistency of low concentrations of TAP loaded into a methylcellulose system in maintaining the antibacterial properties of the original antibiotic mixture.…”

Section: Discussionmentioning

confidence: 99%

“…A creamy mix of TAP that is commonly used clinically was prepared by mixing 1 mL of sterile water with 1,000 mg of equal portions of metronidazole, ciprofloxacin, and minocycline (Champs Pharmacy, San Antonio, TX, USA) (13). A low concentration of TAP with a creamy consistency that can be injected into root canals as inter-appointment medicaments (18) was prepared, as described in recent reports (19,20). Briefly, 2,500, 250, and 25 mg of TAP were dissolved independently in 25 mL of sterile water.…”

Section: Preparation Of Antimicrobials Used In the Studymentioning

confidence: 99%

Direct and indirect antibacterial effects of various concentrations of triple antibiotic pastes loaded in a methylcellulose system

et al. 2016

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We investigated the direct and indirect(residual) antibacterial effects of various concentrations of triple antibiotic paste (TAP) loaded into a methylcellulose system. Enterococcus faecalis (E. faecalis) was grown on sterilized dentin blocks (n = 60) and treated with clinically used TAP (1,000 mg/ mL), low concentrations of methylcellulose-based TAP (100, 10, and 1 mg/mL), placebo paste, or 1.5% NaOCl (n = 10). The pastes were then removed, and biofilm disruption assays were performed. Additional dentin blocks (n = 120) were pretreated with the same experimental groups (n = 20). The pastes were rinsed off, and the samples were immersed independently in phosphate-buffered saline for 2 and 4 weeks (n = 10). E. faecalis was then grown on the dentin blocks, and biofilm disruption assays were performed. Fisher's Exact and Wilcoxon rank sum tests were used for statistical analyses. With regard to direct antibacterial effects, all treatment groups demonstrated complete eradication of biofilms in comparison to placebo paste, while 10 mg/mL of TAP or higher provided substantial residual antibacterial effects. However, dentin treated with 1 mg/mL of TAP or 1.5% NaOCl did not provide substantial residual antibacterial effects. Dentin pretreated with 10 mg/mL of TAP or higher exhibited extended residual antibacterial effects and can thus be used during endodontic regeneration. (J Oral Sci 58, 575-582, 2016)

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“…The low concentrations of DAP (1, 5, and 50 mg/mL) were loaded into a methylcellulose system as described in previous studies (Tagelsir, et al, 2016;Yassen, Sabrah, Eckert, & Platt, 2015) to create a clinically applicable antibiotic medicament that can be injected into a root canal system (Algarni, Yassen, & Gregory, 2015). In summary, 2500, 250 and 50 mg of DAP were dissolved independently in 50 mL of sterile water.…”

Section: Preparation Of Medicaments Used In the Studymentioning

confidence: 99%

Residual antibiofilm effects of various concentrations of double antibiotic paste used during regenerative endodontics after different application times

Jenks

Ehrlich

Spolnik

et al. 2016

Archives of Oral Biology

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Objective: We investigated the residual antibiofilm effects of different concentrations of double antibiotic paste (DAP) applied on radicular dentin for 1 or 4 weeks.Design: Dentin samples were prepared (n=120), sterilized and pretreated for 1 or 4 weeks with the clinically used concentration of DAP (500 mg/mL), low concentrations of DAP (1, 5 or 50 mg/mL) loaded into a methylcellulose system, calcium hydroxide (Ca(OH) 2 ), or placebo paste.After the assigned treatment time, treatment pastes were rinsed off and the samples were kept independently in phosphate buffered saline for 3 weeks. Pretreated dentin samples were then inoculated with Enterococcus faecalis and bacterial biofilms were allowed to grow for an additional 3 weeks. Biofilms were then retrieved from dentin using biofilm disruption assays, diluted, spiral plated, and quantified. Fisher's Exact and Wilcoxon rank sum tests were used for statistical comparisons (α=0.05). Results:Dentin pretreatment for 4 weeks with 5, 50 or 500 mg/mL of DAP demonstrated significantly higher residual antibiofilm effects and complete eradication of E. faecalis biofilms in comparison to a 1 week pretreatment with similar concentrations. However, dentin pretreated with 1 mg/mL of DAP or Ca(OH) 2 did not provide a substantial residual antibiofilm effect regardless of the application time.Conclusions: Dentin pretreatment with 5 mg/mL of DAP or higher for 4 weeks induced significantly higher residual antibiofilm effects in comparison to a 1 week pretreatment with the same concentrations.

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Effect of Different Endodontic Regeneration Protocols on Wettability, Roughness, and Chemical Composition of Surface Dentin

Cited by 38 publications

References 34 publications

Attachment and proliferation of dental pulp stem cells on dentine treated with different regenerative endodontic protocols

Attachment and proliferation of dental pulp stem cells on dentine treated with different regenerative endodontic protocols

Direct and indirect antibacterial effects of various concentrations of triple antibiotic pastes loaded in a methylcellulose system

Residual antibiofilm effects of various concentrations of double antibiotic paste used during regenerative endodontics after different application times

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