Effect of Different Thawing Rates on Motility and Fertilizing Capacity of Cryopreserved Grass Carp (Ctenopharyngodon Idella) Sperm

Yavaş, İlker; Bozkurt, Yusuf

doi:10.5504/bbeq.2011.0018

Cited by 18 publications

(13 citation statements)

References 26 publications

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“…Therefore, before cryopreservation of sperm, a through evaluation of different extender solutions, cryoprotectants, straw sizes and thawing rates is essential to develop optimum cryopreservation protocols for various species (Yavas & Bozkurt, 2011).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Brown Trout (Salmo trutta macrostigma) and Ornamental Koi Carp (Cyprinus carpio) Sperm

Bozkurt¹,

Yavaş²,

Karaca³

2012

Current Frontiers in Cryopreservation

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Section: Introductionmentioning

confidence: 99%

Cryopreservation of Brown Trout (Salmo trutta macrostigma) and Ornamental Koi Carp (Cyprinus carpio) Sperm

Bozkurt¹,

Yavaş²,

Karaca³

2012

Current Frontiers in Cryopreservation

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“…So, another important reason for the success of higher fertilization results can be linked to the adequate penetration of methanol through cell membranes. This indicates that methanol has stable protective impact against freezing damage as membrane stabilizer (Yavaş and Bozkurt 2011).…”

Section: Discussionmentioning

confidence: 97%

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

Bozkurt

Yavaş

2017

Journal of Limnology and Freshwater Fisheries Research

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Cryopreservation of sperm cells is an essential process applied for long-term conservation of aquatic genetic resources. The goal of this research was to determine effect different of straw volumes and thawing rates on the postthaw quality and fertilization ability of cryopreserved common carp (Cyprinus carpio) sperm. In this study, semen was cryopreserved according to conventional slow freezing protocol. For this aim, the cryosolution contained 75 mM NaCl, 70 mM KCl, 2 mM CaCl2, 1 mM MgSO4 and 20 mM Tris (pH: 8) supplemented with 10% MeOH. Following equilibration at +4 °C for 10 min, semen was packed into 0.25, 0.5 and 1.5 mL straws and frozen in liquid nitrogen vapour (for 10 min at -120 ºC) and finally stored in liquid nitrogen (-196 ºC) tank. Thawing of cryopreserved semen process was performed at 30 ºC for 10, 20 and 30 seconds in a water bath. Fertilization was performed using a ratio of 1x10 5 spermatozoa/egg. The highest fertility (68.4±2.5%) was determined with cryopreserved sperm packed in 1.5 mL straws that thawed at 30 ºC for 30 s. According to the results of this research, sperm cryopreserved with ionic extender containing 10% methanol and packed in 1.5 mL straws are suitable to achieve high fertilization of common carp eggs.

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“…Suhu thawing yang digunakan dalam penelitian ini adalah sebesar 26 o C dengan periode thawing selama 15 detik. Yavas and Bozkurt (2011), menyatakan bahwa suhu dan periode terbaik yang berpengaruh pada motilitas pada saat thawing adalah dengan suhu 35 o C selama 30 detik. Steyn et al (1985) dalam Francis et al (2013) menambahkan bahwa proses thawing harus dilakukan dengan waktu yang singkat untuk menghindari kerusakan sel yang disebabkan oleh rekristalisasi.…”

Section: Jumlah Spermatozoa Yang Motilunclassified

“…Proses thawing pada penelitian ini dilakukan dengan menggunakan suhu 26 o C selama 15 detik. Menurut Yavas and Bozkurt (2011), proses thawing yang terbaik adalah dengan menggunakan suhu 35 o C selama 30 detik pada spermatozoa grass carp. Proses thawing yang tepat dapat mencegah terjadinya pembentukan kembali kristal es (Lahnsteiner, 2000 dalam Yavas and Bozkurt, 2011).…”

Section: Jumlah Spermatozoa Yang Motilunclassified

“…Menurut Yavas and Bozkurt (2011), proses thawing yang terbaik adalah dengan menggunakan suhu 35 o C selama 30 detik pada spermatozoa grass carp. Proses thawing yang tepat dapat mencegah terjadinya pembentukan kembali kristal es (Lahnsteiner, 2000 dalam Yavas and Bozkurt, 2011). Pembentukan kristal es selama proses kriopreservasi sel spermatozoa menyebabkan terjadinya penumpukan elektrolit di dalam sel.…”

Section: Jumlah Spermatozoa Yang Motilunclassified

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Pengaruh Perbedaan Konsentrasi Gliserol dalam Susu Skim Kuning Telur untuk Proses Penyimpanan Sperma Beku terhadap Motilitas dan Viabilitas Spermatozoa Ikan Patin (Pangasius pangasius) [Effect Of Different Glycerol Concentration In Skim Milk And Egg Yolk Diluter For Storage Process Of Frozen Semen On Sperm Motility And Viability Of Catfish (Pangasius pangasius)]

Sudarno

Novianto

Masithah

2014

JIPK

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Needs of catfish seed always increase every years, especially for farming activities. Fulfillment demand of seed in large quntities and continuing are the main obstacle in the production process of catfish. This can be overcome by the process of cryopreservation. This process requires diluents and cryoprotectants to maintain the fertility of spermatozoa. Skim milk and egg yolks diluents has been used on fish because it was applied to carp and showing a good result of sperm motility and fertilization rate. Glycerol as cryoprotectant was reported effective to be used for European Catfish than the other kind cryoprotectant. This study aimed to determine the effect of different concentrations of glycerol in the diluent skim milk and egg yolk on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. This research method is the experiment with completely randomized design (CRD) as the experimental design. The treatment used is different glycerol concentrations, P1 (11%), P2 (13%), P3 (15%), and P4 (17%) and each of the treatment was repeated 5 times. The main parameters measured were motility (%) and viability (%) of spermatozoa. Supporting parameters include odor, color, pH, viscosity, sperm concentration and catfish (Pangasius pangasius). Analysis of the data using Analysis Of Variance (ANOVA) and to determine the best treatment performed Duncan's Multiple Range Test with 95% confidance interval. The results showed that the effect of the concentration of glycerol in the diluent skim milk and egg yolk was not significantly (P> 0.05) on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. Further testing needs to be done on the level of fertility of frozen sperm, as well as the growth rate of seeds produced from sperm frozen catfish (Pangasius pangasius) products

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Effect of Different Thawing Rates on Motility and Fertilizing Capacity of Cryopreserved Grass Carp (Ctenopharyngodon Idella) Sperm

Cited by 18 publications

References 26 publications

Cryopreservation of Brown Trout (Salmo trutta macrostigma) and Ornamental Koi Carp (Cyprinus carpio) Sperm

Cryopreservation of Brown Trout (Salmo trutta macrostigma) and Ornamental Koi Carp (Cyprinus carpio) Sperm

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

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