Effect of docosahexaenoic acid in a mouse facial nerve injury model

Abstract

We have previously reported in a model of spinal cord injury that the omega-3 polyunsaturated fatty acid docosahexaenoic acid (DHA) has signifi cant neuroprotective effects after trauma in the central nervous system [1]. In the present study we extend our observations on the neuroprotective and neuroregenerative effects of DHA to a peripheral nerve injury model. Facial nerve transection in mice is a well-established model used in studies on axonal regeneration [2]. We carried out this nerve lesion in mice and we studied the effects of oral supplementation with DHA after injury, on neuronal and glial markers of injury, and on neurological outcome. Female adult C57Bl6 mice (6 animals per group) were used throughout. Animals were placed for 1 week before surgery on a standard powdered diet. For the surgery, the right facial nerve, including the retroauricular branch, was transected at the styloid foramen under deep anaesthesia. After injury, mice continued to receive either the standard diet or a diet enriched with DHA (300 mg/kg/day), for 14 days. On the last day of treatment, the neurological outcome was assessed by measuring the movement of the whiskers, on a scale from 0 (no movement) to 3 (normal movement) [3]. Then the animals were killed and perfused intracardially with 4% paraformaldehyde. The brainstem was removed, post-fi xed and cryoprotected. 20 µm sections were cut at the level of the facial nucleus, and were incubated with primary antisera for calcitonin gene-related peptide (CGRP, 1:400, peninsula) and galanin (1:400, Peninsula), as neuronal markers, and for the integrin subunits αM (1:5000, Pharmingen) and β1 (1:3000, Pharmingen), whose expression is microglial only, and mixed neuronal/microglial, respectively. Total immunoreactivity for each antigen was quantifi ed in 2 sections per animal. Data was expressed as means ± SEM and analysed statistically using Student's t test. In the DHA-treated group there was a signifi cant increase in the number of both CGRP-and galanin-immunoreactive sprouts, vs. the lesioned control animals kept on the normal diet (+ 140% and + 90%, respectively, both p < 0.05). A signifi cant increase was also seen in integrin immunoreactivity, with an increase of 20% for the αM-, and 167% for the β1-integrin subunit, respectively (both p < 0.05) in animals receiving the DHA-supplemented diet, in comparison with animals on the control diet. The intake of DHA after injury also led to a better functional outcome, although the increase in whisker mobility 14 days after injury (+ 67% vs. controls) was not statistically signifi cant. These results show that the dietary supplementation with DHA in the period following facial nerve injury has significant effects on the response of both neurons and microglia to injury, and appears to promote a pro-regenerative response. This suggests that DHA could have a neuroprotective potential in the treatment of peripheral nerve injury.

Improved Outcome after Peripheral Nerve Injury in Mice with Increased Levels of Endogenous Omega-3 Polyunsaturated Fatty Acids

“…One aspect concerning the use of -3 PUFAs in neurotrauma that remains to be fully explored is their potential neuroprotective and pro-regenerative effect after PNI. However, -3 PUFAs can enhance neurite outgrowth in cultures of hippocampal and cortical neurons (Calderon and Kim, 2004), as well as in cultures of primary sensory neurons (Robson et al, 2010), and DHA has been shown to increase the number of sprouting fibers after a facial nerve transection (Makwana et al, 2006).…”