Effect of duration and temperature of storage on serum analyte stability: examination of 14 selected radioimmunoassay procedures.

Kubasik, Norman P.; Ricotta, M; Hunter, Thomas H.; Sine, H E

doi:10.1093/clinchem/28.1.164

Cited by 43 publications

(8 citation statements)

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“…Studies designed to examine the effects of storage time on serum/plasma levels of steroid hormones have compared levels of cortisol and testosterone from the same samples immediately after collection and after years of storage. In these investigations, testosterone and cortisol concentrations were stable at levels consistent with typical coefficients of variation after up to 10-11 years of storage (Wickings and Nieschlag, 1976;Kubasik et al, 1982;Das et al, 1983;Kley and Rick, 1984;Kley et al, 1985;Diver et al, 1994;Bolelli et al, 1995;Bogdan, 2000, Personal Communication). Other studies have examined effects of variation in storage conditions (e.g., repeated freeze-thaw cycles, storage at varied temperatures) on cortisol and testosterone concentrations (Wickings and Nieschlag, 1976;Kubasik et al, 1982;Das et al, 1983;Kley and Rick, 1984;Garde and Hansen, 2005) as well as CBG and SHBG (Kley and Rick, 1984;Sinnecker, 1989;Reyna et al, 2001).…”

Section: Introductionmentioning

confidence: 53%

Long-term stability of maternal prenatal steroid hormones from the National Collaborative Perinatal Project: Still valid after all these years

Stroud

Solomon

Shenassa

et al. 2007

Psychoneuroendocrinology

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Large epidemiological samples, including the National Collaborative Perinatal Project (NCPP), in which blood/serum was collected during pregnancy and offspring followed longitudinally, offer the unique opportunity to examine neuroendocrine mechanisms underlying prenatal "programming" of adult health and disease. However, in order to conduct longitudinal analyses, it is critical to determine the validity of maternal prenatal samples stored over long periods. We investigated the validity of cortisol, testosterone, and their binding globulins (corticosteroid-binding globulin (CBG) and sex hormone-binding globulin (SHBG)) in maternal prenatal serum from the NCPP after over 40 years of storage. Study 1 included 64 maternal serum samples collected on the day of delivery; study 2 involved 1099 third trimester serum samples collected between gestational weeks 31 and 36. Across both studies, cortisol and testosterone concentrations were consistent with values from published studies of fresh samples collected at similar points in gestation. CBG and SHBG were present, but showed some differences from published studies. Results support the validity of cortisol and testosterone values following 40+ years of storage. Results also provide validation for future longitudinal tests of prenatal "programming" hypotheses within the NCPP. Stability of steroid hormones over decades suggests that stored samples from other longitudinal studies may also allow opportunities to investigate links between prenatal steroids and long-term offspring outcomes.

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Section: Introductionmentioning

confidence: 53%

Long-term stability of maternal prenatal steroid hormones from the National Collaborative Perinatal Project: Still valid after all these years

Stroud

Solomon

Shenassa

et al. 2007

Psychoneuroendocrinology

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“…Finally, plasma samples used for this study had been stored at −80 ° C for 6 years without undergoing any thaw cycles. Use of stored samples may be seen as a limitation; however, numerous publications attesting to the high stability of steroid and thyroid hormones when preserved at −80 °C have been published (Kubasik et al, 1982; Kley, Schlaghecke & Krüskemper, 1985; Garde & Hansen, 2005; EL Ezzi, El-Saidi & Kuddus, 2010; Hillebrand, Heijboer & Endert, 2017).…”

Section: Discussionmentioning

confidence: 99%

Cortisol, progesterone, 17α-hydroxyprogesterone, and TSH responses in dogs injected with low-dose lipopolysaccharide

Corder-Ramos

Flatland

Fry

et al. 2019

PeerJ

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Background Stress and diseases such as endotoxemia induce cortisol synthesis through a complex biosynthetic pathway involving intermediates (progesterone, and 17α-hydroxyprogesterone (17α-OHP)) and suppression of the hypothalamus-pituitary-thyroid axis. Objective To measure plasma concentrations of cortisol, progesterone, 17α-OHP, and thyroid stimulating hormone (TSH) in dogs experimentally injected with intravenous low-dose lipopolysaccharide (LPS). Our hypothesis was that LPS treatment would elicit a significant increase in cortisol and its precursors, and a significant decrease in TSH concentration. Methods Hormone measurements were performed on blood samples left over from a previous investigation (2011) on the effect of low-dose LPS on hematological measurands. Five sexually intact female dogs, none in estrous at the time of the study, were administered saline treatment two weeks prior to LPS treatment. LPS was administered intravenously at a dose of 0.1 µg/kg. Blood was collected before (baseline, time -24 hours) and 3-, 6- and 24-hours post-injection. Mixed model analysis for repeated measures was used, with both treatment and time as the repeated factors. Ranked transformation were applied when diagnostic analysis exhibited violation of normality and equal variance assumptions. Post hoc multiple comparisons were performed with Tukey’s adjustment. Statistical significance was defined as p < 0.05. Results Significant differences relative to baseline values were detected following both treatments. Compared to baseline, dogs had significantly higher cortisol and 17α-OHP at 3-hours, and significantly lower TSH at 3- and 6-hours following LPS treatment. Dogs had significantly lower TSH at 6- and 24- following saline treatment. Though not statistically significant, the trend in progesterone concentrations was similar to cortisol and 17α-OHP, with an increase at 3-hours post-injection followed by a decrease close to baseline following both LPS and saline. Cortisol and 17α-OHP concentrations were higher after LPS treatment than after saline treatment at 3- and 6-hours post-injection, but differences were not statistically significant, and no significant differences between treatments were detected for any other hormone or timepoint. Discussion and conclusion Cortisol and its adrenal precursors are released in the bloodstream following a low dose of LPS, while TSH appears to decrease. Similar changes occurred following saline treatment, suggesting that even routine handling and saline injection in conditioned dogs can elicit alterations in the internal equilibrium with subsequent modification of both hypothalamus-pituitary-adrenal and thyroid axes. Changes to adrenal and thyroid hormone concentrations must be interpreted in light of clinical information. Further studies are needed to elucidate mechanisms of adrenal steroidal hormone synthesis and secretion in response to various stressful stimuli in both neutered and intact animals.

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“…[13][14][15] However, using sample preservatives for research sample collection is inconvenient and adds to time and expense [20] and similarly some preservatives such as glycerol are unsuitable for anti-doping purpose. Previous studies have reported that plasma LH is stable after storage for 8 and 14 days after refrigeration (4 C), [39,40] for 14 days stored frozen (-6 C) [40] and for up to 9 months after -20 or -70 C storage. [41] The quantitative reproducibility of the ICL immunoassay indicates it is more robust for measurements of urinary LH for anti-doping purposes compared with the IF assay.…”

Section: Discussionmentioning

confidence: 99%

Immunoreactive LH in long‐term frozen human urine samples

Singh

Jimenez

Newman

et al. 2013

Drug Testing and Analysis

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Urine provides a convenient non-invasive alternative to blood sampling for measurement of certain hormones. Urinary luteinizing hormone (LH) measurements have been used for endocrinology research and anti-doping testing. However, the commercially available LH immunoassays are developed and validated for human blood samples but not urine so that LH assays intended for use with urine samples need thorough validation. Therefore, the present study evaluated the measurement of urinary LH immunoreactivity using previously validated immunofluorometric (IF) and immunochemiluminometric (ICL) LH assays after prolonged frozen storage. LH was measured in serial urine samples following administration of a single injection of one of two doses of recombinant human chorionic hormone (rhCG) with assays run at the end of study (2008) and again after four years of frozen (-20 °C) storage where samples were stored without adding preservatives. The ICL assay showed quantitatively reproducible LH measurements after prolonged -20 °C storage. However, the IF immunoassay gave consistently lower LH levels relative to ICL (2008) with a further proportionate reduction after four years of sample storage (2012). Yet, both the assays displayed similar patterns of the time-course of urine LH measurement both before and after four years of frozen storage. In conclusion, we found that both immunoassays are suitable for urinary LH measurements with ICL assay being more robust for quantitative urinary LH measurement such as for anti-doping purposes, whereas the IF could be applicable for research studies where urine LH levels are compared within-study but not in absolute terms.

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Effect of duration and temperature of storage on serum analyte stability: examination of 14 selected radioimmunoassay procedures.

Cited by 43 publications

References 0 publications

Long-term stability of maternal prenatal steroid hormones from the National Collaborative Perinatal Project: Still valid after all these years

Long-term stability of maternal prenatal steroid hormones from the National Collaborative Perinatal Project: Still valid after all these years

Cortisol, progesterone, 17α-hydroxyprogesterone, and TSH responses in dogs injected with low-dose lipopolysaccharide

Immunoreactive LH in long‐term frozen human urine samples

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