Effect of electroosmosis on detection in isotachophoresis

Reijenga, JC Jetse; Aben, Gva; Verheggen, Tpem Theo; Everaerts, FM Frans

doi:10.1016/0021-9673(83)80034-x

Cited by 135 publications

(41 citation statements)

References 3 publications

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“…The increase in viscosity will be partly compensated by Joule heating with a higher electric current (see also section 3.1., Applied field strength). Here it should be noted that in capillary electrophoretic techniques a distinction can be made between bulk viscosity (important for muc ) and wall-surface viscosity (important for mEOF) [24]. We assume that bulk viscosity will be more influenced by Joule heating than wall surface viscosity.…”

Section: Surfactant Concentrationmentioning

confidence: 99%

Parameters controlling the elution window and retention factors in micellar electrokinetic capillary chromatography

Muijselaar

Claessens

Cramers

1995

Journal of Chromatography A

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Section: Surfactant Concentrationmentioning

confidence: 99%

Parameters controlling the elution window and retention factors in micellar electrokinetic capillary chromatography

Muijselaar

Claessens

Cramers

1995

Journal of Chromatography A

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“…Cetyltrimethylammonium bromide (CTAB) is known to decrease and even change the sign of the c-potential of the capillary wall [12]. By adding a small concentration (1.…”

Section: Resultsmentioning

confidence: 99%

Equipment for multifunctional use in high-performance capillary electrophoresis

Verheggen

Everaerts

1993

Journal of Chromatography A

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“…[20][21][22][23][24] We tested three different capillaries in this study. One of them was recovered with PVA, a highly hydrophilic polymer covalently bound to the capillary surface.…”

Section: Capillary Inner Coatingsmentioning

confidence: 99%

Evaluation of capillaries with different inner coatings for DNA analysis using dilute polymer solutions by capillary electrophoresis

Eugênio¹,

Carrilho²

2009

J. Braz. Chem. Soc.

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No presente trabalho, três colunas capilares, uma sem recobrimento interno e duas com recobrimentos internos diferentes foram utilizadas na separação de fragmentos de DNA: poli(vinil álcool) (PVA) e poli(dimetilacrilamida) (PDMA) -ambos de recobrimento covalente -foram comparados para a separação de DNA utilizando soluções poliméricas. As separações foram realizadas usando hidroxietilcelulose (HEC) (90-105 kDa) nas concentrações entre 0,00 e 2,00% m/v. Os resultados indicaram que a eficiência de separação foi maior no capilar de PVA do que no de PDMA, em todas as concentrações de HEC testadas. Ainda, uma resolução superior também foi observada com o capilar de PVA, já que com o capilar de PDMA o formato dos picos não se mostrou reprodutível quando corridas subseqüentes foram realizadas. Contrariamente ao relatado na literatura, nenhuma separação foi conseguida com o capilar sem revestimento interno.In this work three capillary columns, one with uncoated inner wall and two with covalentlybound internal coatings -poly(vinyl alcohol) (PVA) and poly(dimethylacrylamide) (PDMA) -both covalently covered -were used to separate DNA fragments and compared to DNA separation using replaceable polymer solutions. The separations were performed using hydroxyethylcellulose (HEC) (90-105 kDa) in concentrations ranging from 0.00 to 2.00% m/v. The results indicated that the separation efficiency was higher in the PVA capillary than in the PDMA in all evaluated concentrations of HEC. In addition, higher resolution was also observed in PVA-coated capillary since in PDMA the shape of the peaks was not reproducible when subsequent runs were performed. Contrary to what has previously been reported in the literature, no reasonable separation was possible in bare fused silica.Keywords: DNA separation, capillary coating, laser-induced fluorescence, PDMA, PVA IntroductionCapillary electrophoresis (CE) is a separation technique widely used for separation of biological macromolecules. It offers great advantages over slab-gel electrophoresis (SGE) -shorter analysis time, smaller quantity of sample, greater efficiency of separation, and easier automation. 1,2 The first attempts to apply CE to DNA separations were carried out in capillaries filled with cross-linked gels, like polyacrylamide. [3][4][5] Later on, some polymeric solutions were used, known as dynamic or physical gels, which are easily prepared and introduced into the capillary, thus enabling its renewal after each analysis and the use of a single capillary for many separations, without any problem with the subsequent separations. 6 DNA migration mechanisms in polymeric matricesThe Ogston and the reptation models were first proposed to explain DNA migration in slab gels, but they have also shown themselves suitable for polymeric solutions. 7 According to the Ogston theory, the DNA fragment keeps a spherical and rigid conformation of radius Rg. This particle moves across a random net formed by the gel fibers. These fibers form pores with average size ξ, which is a function of the po...

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Effect of electroosmosis on detection in isotachophoresis

Cited by 135 publications

References 3 publications

Parameters controlling the elution window and retention factors in micellar electrokinetic capillary chromatography

Parameters controlling the elution window and retention factors in micellar electrokinetic capillary chromatography

Equipment for multifunctional use in high-performance capillary electrophoresis

Evaluation of capillaries with different inner coatings for DNA analysis using dilute polymer solutions by capillary electrophoresis

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