Effect of Elicitation on Polyphenol and Carotenoid Metabolism in Butterhead Lettuce (Lactuca sativa var. capitata)

Abstract

The effect of elicitation in butterhead lettuce on carotenoid and polyphenol metabolism was evaluated. Different concentrations of arachidonic acid (AA), salicylic acid (SA), methyl jasmonate (MJ) (15, 45, and 90 μM) and Harpin protein (HP) (30, 60, and 120 mg/L) were applied on red and green butterhead lettuces. Total phenolic and flavonoid content were incremented by MJ (90 μM) in green and red lettuce. Carotenoids were increased in red lettuce (AA; 45 μM). Green lettuce modifies their phenolic acid profile after elicitation with AA and MJ; meanwhile, red lettuce incremented mainly in hydroxycinnamic acids and flavonols, MJ being the elicitor with the highest effect. There was an impact on secondary metabolite enzyme gene transcript concentration. Phenylalanine ammonia-lyase (PAL) and lycopene beta cyclase (LBC) increased in both varieties after elicitation. A relationship between phytochemical increase and the activation of the metabolic pathways after elicitation in butterhead lettuce was observed.

Grapevine Stimulation: A Multidisciplinary Approach to Investigate the Effects of Biostimulants and a Plant Defense Stimulator

“…However, anthocyanin and flavonol contents increased significantly in BS-treated leaves compared to ASM-treated leaves. These results suggested that ASM did not promote the synthesis of flavonols such as quercetin and derivatives, or that other molecules (flavanols) such as catechin and derivatives were less well-detected at the wavelength used with the Dualex clip (480 nm) (Dr. Cluzet S. personal communication), or even that as yet unknown post-transcriptional phenomena might limit the biosynthesis to promote another pathway. A recent study comparing the effects of various elicitors on lettuce confirmed the role of SA in favor of flavanol biosynthesis .…”

“…These results suggested that ASM did not promote the synthesis of flavonols such as quercetin and derivatives, or that other molecules (flavanols) such as catechin and derivatives were less well-detected at the wavelength used with the Dualex clip (480 nm) (Dr. Cluzet S. personal communication), or even that as yet unknown post-transcriptional phenomena might limit the biosynthesis to promote another pathway. A recent study comparing the effects of various elicitors on lettuce confirmed the role of SA in favor of flavanol biosynthesis . Concerning the biosynthesis of phenylpropanoids, it was surprising to observe no overexpression of VvPAL and VvSTS in ASM-treated leaves, suggesting that VvSTS was probably already overexpressed in the latter.…”

“…A recent study comparing the effects of various elicitors on lettuce confirmed the role of SA in favor of flavanol biosynthesis. 30 Concerning the biosynthesis of phenylpropanoids, it was surprising to observe no overexpression of VvPAL and VvSTS in ASM-treated leaves, suggesting that VvSTS was probably already overexpressed in the latter. However, VvPAL was overexpressed at 2 dpt in SWtreated leaves.…”

Assessment of transcriptional reprogramming of lettuce roots in response to chitin soil amendment

“…We were, however, able to detect higher levels of defense-associated metabolites in both leaf and root tissues, such as SA. Furthermore, SA is known to increase flavonoid production in lettuce ( Moreno-Escamilla et al., 2020 ). This finding is congruent with our results of the flavonoid pathway being upregulated in the roots, higher flavonoid levels in the leaves within the first two weeks and higher levels of SA upon chitin treatment.…”

PENGARUH ELISITOR KITOSAN TERHADAP KANDUNGAN WITHANOLID TUNAS IN VITRO AKSESI TANAMAN Physalis angulata DARI PULAU MADURA

“…Bahkan elisitor sodium selenat tidak menunjukkan perbedaan yang signifikan dengan kontrol (Barrientos Carvacho et al, 2014). Elisitor pada konsentrasi tinggi dapat menginduksi akumulasi ROS yang lebih tinggi sehingga menyebabkan kematian sel yang dapat mengakibatkan penurunan fitokimia (Moreno-Escamilla et al, 2020). Pada penelitian ini kitosan 125 mg/L diduga menurunkan ketahanan sel tunas in vitro hasil regenerasi eksplan nodus yang berasal dari Pamekasan (A5) dan berpengaruh terhadap kemampuan sintesis dan akumulasi senyawa withanolid.…”