Effect of endophytic bacterium, Stenotrophomonas maltophilia JVB5 on sunflowers

Abstract

Identifiable endophytic bacteria with plant growth-promoting traits promise to ensure sustainable agriculture. However, information on the versatility and exploration of sunflower-associated bacteria as bioinoculants is less studied. Here, we present the whole-genome sequence and annotation of Stenotrophomonas maltophilia JVB5 isolated from the sunflower root endosphere from the North West province, South Africa. The whole-genome analysis revealed a genome size of 4 771 305 bp, a sequence read count of 8 764 890, a 66% guanine-cytosine content, 57 tRNAs, 268 contigs, and 4 160 protein-coding genes with functions in various metabolic pathways. Pathways involved in the indole acetic acid production were found in the S. maltophilia JVB5 genome. The whole-genome annotation predicted notable genes involved in bacterial colonisation, antibiosis, and plant growth promotion. The predicted genes are involved in the sulfur metabolism, and the oxidative stress may enhance the plant growth promotion and boost plant the resistance to stress. Upon inoculation, S. maltophilia JVB5 efficiently colonised the sunflower root under greenhouse conditions with a significant improvement on the wet plant weight of 437.20 g compared to the uninoculated control with a 331.04 g wet weight. The genomic analysis revealing specific functional genes in the bacteria genome suggests their bioprospecting in agriculture. Hence, understanding the mechanisms employed by S. maltophilia JVB5 based on the predicted multifunctional genes will help harness their bioresource in sustainable plant health.

Bioprospecting native endophytic and epiphytic diazotrophs of Oryza sativa (L) for plant growth promotion and biocontrol

“…The isolated bacteria belonged to three phyla namely, Proteobacteria (seven bacterial isolates), Firmicutes (two bacterial isolates) and Actinobacteria (one bacterial isolate). However, diazotropic diversity reported in our study was found to be similar to the results of earlier researchers among various species of genera such as Bacillus, Stenotrophomonas, Pseudomonas, Microbacterium in rice, maize, sun ower, tuber and root crops (Ki e and Laing 2016, Duque and Villordon 2019, Adeleke et al 2022. Two rhizospheric bacterial isolates M. subterraneus and B. nasdae, were not reported earlier as rhizospheric bacterial isolates while their rst isolation was from the Australian thermal aquifer and condensation water from Mir, Russian space station respectively (Tan et al 2017, Li et al 2017.…”

Bioprospecting and Challenges of Plant Microbiome Research for Sustainable Agriculture, a Review on Soybean Endophytic Bacteria

“…Better still, there is a need to develop robust bioinformatics tools and analytical techniques with the existing technologies to generate microbiome data as a guide for further experiments. Adeleke et al [ 40 ] and Adeleke et al [ 157 ] reported the genomic characterization of plant growth-promoting endophytic bacteria, Bacillus cereus T4S and Stenotrophomonas maltophilia JVB5 as copious sunflower growth enhancers. Furthermore, effort on the use of these endophytic bacteria as biocontrol agent against phytopathogens is expected to be investigated in the future studies.…”

Synthesis of Chitosan Microparticles Encapsulating Bacterial Cell-Free Supernatants and Indole Acetic Acid, and Their Effects on Germination and Seedling Growth in Tomato (Solanum lycopersicum)

“…Te concentration of IAA produced by the S. maltophilia strain is considered low. However, the addition of diferent concentrations of L-trp contributed signifcantly to the increase in IAA production, ranging from 3.9 μg/mL to 2.5 mg/mL [30,[32][33][34]36]. Growth of the bacterium in LB medium spiked with L-trp as a precursor at a concentration of 25 ug/mL produced a 24% increase in IAA within 24 h. It was also observed that higher concentrations (100-400 ug/ mL) of the precursor increased IAA production to 79 ± 2.…”

“…It has been shown that S. maltophilia can promote plant growth, stimulate pathogen control, and increase plant tolerance to different types of stresses due to the production of phytohormones such as IAA and protective enzymes [ 35 ]. Adeleke et al [ 36 ] demonstrated that genes such as trp ABCD, ami E, and mia A present in this bacterium were related to IAA synthesis through the indole-3-acetamine pathway.…”