Effect of entomopoxvirus infection of the smaller tea tortrix, <i>Adoxophyes</i> sp. on the development of the endoparasitoid, <i>Ascogaster reticulatus</i>

Nakai, M.; Sakai, Takuro; Kunimi, Yasuhisa

doi:10.1046/j.1570-7458.1997.00194.x

Cited by 19 publications

(8 citation statements)

References 18 publications

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“…Therefore, as a result, in the dually parasitized and Splt-NPV-infected hosts, the emergence rates of parasitoid larvae from the hosts inoculated with 10ϫ the LC 95 of SpltNPV were lower than those from the hosts inoculated with the LC 95 of SpltNPV. Similarly, Nakai et al (1997) reported that when the neonate larvae of Adoxophyes honmai (Lepidoptera: Tortricidae) parasitized by Ascogaster reticulatus (Hymenoptera: Braconidae) were infected with an entomopoxvirus (EPV), survival of the parasitoid larvae developing in the hosts that were exposed to a high concentration of EPV was lower than for those developing in hosts that were exposed to a low concentration of EPV. This suggests that the higher dose of inoculation shortens the survival time of the host, and parasitoids do not have enough time to complete their development in hosts infected with the higher dose of viruses.…”

Section: Discussionmentioning

confidence: 99%

Interaction between a nucleopolyhedrovirus and the braconid parasitoid Meteorus pulchricornis (Hymenoptera: Braconidae) in the larvae of Spodoptera litura (Lepidoptera: Noctuidae)

Nguyen

Nakai

Takatsuka

et al. 2005

Appl. Entomol. Zool.

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We investigated the survival and development of the braconid parasitoid Meteorus pulchricornis in nucleopolyhedrovirus (NPV)-infected Spodoptera litura larvae. The first and second molting stages of the larvae were parasitized by M. pulchricornis and were exposed to a 95% lethal concentration (LC 95 ) and 10ϫ the LC 95 of S. litura NPV (Splt-NPV) on the first, third, and fifth day post-parasitization (dpp). Infection of the larvae with SpltNPV was deleterious to the survival and development of M. pulchricornis. The survival of M. pulchricornis in SpltNPV-infected S. litura larvae was dependent on the interval between parasitization and viral infection, as well as on the inoculation dose of SpltNPV; few parasitoid larvae emerged from infected hosts when host larvae were exposed to 10ϫ the LC 95 of Splt-NPV on 1 and 3 dpp. The fecundity of M. pulchricornis that emerged from SpltNPV-infected hosts was not affected, and these parasitoids did not transmit the virus to other hosts. The number of eggs laid by M. pulchricornis females was not significantly different between the SpltNPV-infected and non-infected larvae during the first 4 d post-infection (dpi). On the other hand, at 5 dpi, M. pulchricornis females laid significantly more eggs in non-infected larvae than in SpltNPV-infected larvae.

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Section: Discussionmentioning

confidence: 99%

Interaction between a nucleopolyhedrovirus and the braconid parasitoid Meteorus pulchricornis (Hymenoptera: Braconidae) in the larvae of Spodoptera litura (Lepidoptera: Noctuidae)

Nguyen

Nakai

Takatsuka

et al. 2005

Appl. Entomol. Zool.

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“…AHEV, CBEV, CREV, and MySEV were isolated from diseased larvae of Adoxophyes honmai (collected from a tea field in Tokyo, Japan) (19,20), Choristoneura biennis (from the province of Ontario, Canada) (21), Choristoneura rosaceana (collected in Eastern Canada), and Mythimna separata (obtained from Fulin Sun, Chinese Center for Virus Culture Collection, Wuhan, China) (22), respectively. Viruses were propagated in their respective hosts except for CBEV, which was propagated in Choristoneura fumiferana.…”

Section: Methodsmentioning

confidence: 99%

“…OBs were purified by homogenization and density gradient centrifugation using a 0.25 M sucrose-Percoll (GE Healthcare) solution (19). The purified OB suspensions were dissolved with an alkali buffer containing a reducing agent (1 M sodium carbonate and 0.4 M sodium thioglycolate).…”

Section: Methodsmentioning

confidence: 99%

“…Field studies on important Asian and North American lepidopteran pests revealed that EPVs could be found in diseased larvae of the smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae) (19,20), the 2-year-cycle budworm moth, Choristoneura biennis (Lepidoptera: Tortricidae) (21), the oblique-banded leafroller moth Choristoneura rosaceana (Lepidoptera: Tortricidae), and the oriental armyworm, Mythimna separata (Lepidoptera: Noctuidae) (22). In contrast to baculoviruses, which can kill insect hosts shortly after infection and could be used in place of a chemical insecticide (23), EPVs are slow-acting pathogens and may be more appropriate for reducing the growth rate of the pest population via epizootics that affect the frequency of insect outbreaks.…”

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New Insights into the Evolution of Entomopoxvirinae from the Complete Genome Sequences of Four Entomopoxviruses Infecting Adoxophyes honmai, Choristoneura biennis, Choristoneura rosaceana, and Mythimna separata

Thézé

Takatsuka

Li³

et al. 2013

J Virol

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dPoxviruses are nucleocytoplasmic large DNA viruses encompassing two subfamilies, the Chordopoxvirinae and the Entomopoxvirinae, infecting vertebrates and insects, respectively. While chordopoxvirus genomics have been widely studied, only two entomopoxvirus (EPV) genomes have been entirely sequenced. We report the genome sequences of four EPVs of the Betaentomopoxvirus genus infecting the Lepidoptera: Adoxophyes honmai EPV (AHEV), Choristoneura biennis EPV (CBEV), Choristoneura rosaceana EPV (CREV), and Mythimna separata EPV (MySEV). The genomes are 80% AT rich, are 228 to 307 kbp long, and contain 247 to 334 open reading frames (ORFs). Most genes are homologous to those of Amsacta moorei entomopoxvirus and encode several protein families repeated in tandem in terminal regions. Some genomes also encode proteins of unknown functions with similarity to those of other insect viruses. Comparative genomic analyses highlight a high colinearity among the lepidopteran EPV genomes and little gene order conservation with other poxvirus genomes. As with previously sequenced EPVs, the genomes include a relatively conserved central region flanked by inverted terminal repeats. Protein clustering identified 104 core EPV genes. Among betaentomopoxviruses, 148 core genes were found in relatively high synteny, pointing to low genomic diversity. Whole-genome and spheroidin gene phylogenetic analyses showed that the lepidopteran EPVs group closely in a monophyletic lineage, corroborating their affiliation with the Betaentomopoxvirus genus as well as a clear division of the EPVs according to the orders of insect hosts (Lepidoptera, Coleoptera, and Orthoptera). This suggests an ancient coevolution of EPVs with their insect hosts and the need to revise the current EPV taxonomy to separate orthopteran EPVs from the lepidopteran-specific betaentomopoxviruses so as to form a new genus. Poxviruses are large double-stranded DNA (dsDNA) viruses infecting a wide range of animals. They belong to the phylogenetically related group of viruses termed nucleocytoplasmic large DNA viruses (NCLDV) (1). They harbor linear dsDNA genomes with inverted terminal repeats (ITRs) (2). Poxvirus genomes are 130 to 375 kbp long and replicate in the cytoplasm (3). The family Poxviridae includes two subfamilies: the Chordopoxvirinae, infecting vertebrates, and the Entomopoxvirinae, infecting insects. The chordopoxviruses are classified into nine genera, including Orthopoxvirus and Avipoxvirus (4), and have been the subjects of the main body of research on poxviruses (5, 6). The entomopoxviruses (EPVs) are currently divided into three genera based on host range and virion morphology: Alphaentomopoxvirus, infecting coleopterans; Betaentomopoxvirus, infecting lepidopterans and orthopterans; and Gammaentomopoxvirus, infecting dipterans (4). However, the lack of genomic data has precluded the integration of unifying genetic criteria into this classification. That is why the orthopteran EPV Melanoplus sanguinipes entomopoxvirus was removed from the Betaentomopoxvirus ...

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“…Immature parasitoids often cannot complete their development if their hosts are infected with a virus because of premature death of the host (Brooks 1993; Nakai et al. 1997; Nakai and Kunimi 1998).…”

Section: Discussionmentioning

confidence: 99%

Interactions between an ectoparasitoid and a nucleopolyhedrovirus when simultaneously attacking Spodoptera exigua (Lepidoptera: Noctuidae)

Stoianova

Williams

Cisneros

et al. 2011

J Applied Entomology

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Insect pathogenic viruses and parasitoids represent distinct biological entities that exploit a shared host resource and have similar effects in suppressing host populations. This study explores the interactions between the ectoparasitoid Euplectrus plathypenae (Hymenoptera: Eulophidae) and the Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) in larvae of S. exigua (Lepidoptera: Noctuidae). Parasitoid progeny failed to complete development in hosts that had been infected prior to parasitism. However, infection of S. exigua fourth instars at 48 h post‐parasitism had no significant effects on the survival of parasitoid progeny. Larval and pupal development times of E. plathypenae that survived on virus‐infected S. exigua did not differ significantly from that of parasitoids on healthy hosts. Virus‐induced mortality and the production of occlusion bodies were very similar in parasitized and non‐parasitized S. exigua. The virus was genetically stable over three passages in parasitized and unparasitized hosts. These results suggest that applications of SeMNPV‐based insecticides are unlikely to disrupt pest control exerted by the parasitoid E. plathypenae in biological pest control programs as long as virus applications are timed not to coincide with parasitoid releases.

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Effect of entomopoxvirus infection of the smaller tea tortrix, Adoxophyes sp. on the development of the endoparasitoid, Ascogaster reticulatus

Cited by 19 publications

References 18 publications

Interaction between a nucleopolyhedrovirus and the braconid parasitoid Meteorus pulchricornis (Hymenoptera: Braconidae) in the larvae of Spodoptera litura (Lepidoptera: Noctuidae)

Interaction between a nucleopolyhedrovirus and the braconid parasitoid Meteorus pulchricornis (Hymenoptera: Braconidae) in the larvae of Spodoptera litura (Lepidoptera: Noctuidae)

New Insights into the Evolution of Entomopoxvirinae from the Complete Genome Sequences of Four Entomopoxviruses Infecting Adoxophyes honmai, Choristoneura biennis, Choristoneura rosaceana, and Mythimna separata

Interactions between an ectoparasitoid and a nucleopolyhedrovirus when simultaneously attacking Spodoptera exigua (Lepidoptera: Noctuidae)

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