Effect of enzyme supplementation to dehulled lupin-based diets on growth, feed efficiency, nutrient digestibility and carcass composition of rainbow trout, Oncorhynchus mykiss (Walbaum)

Abstract

Effect of enzyme supplementation to dehulled lupin-based diets on growth, feed efficiency, nutrient digestibility and carcass composition of rainbow trout, Abstract High inclusion levels of dehulled lupin (Lupinus angustifolius) in salmonid diets signi¢cantly decrease growth rates. This may be caused by the high concentrations of non-starch polysaccharides including oligosaccharide (OS) in lupin. The antinutritive e¡ects of OS have not yet been fully investigated in ¢sh. The objective of this study was to determine the e¡ect of enzyme supplementation of dehulled lupin-based diets on the ¢sh performance. There were two control diets: a ¢sh meal-based diet with no plant protein (FM) and a diet that contained 50% dehulled lupin (L). Four experimental diets based on diet L and containing four di¡erent exogenous enzyme supplements were used: diet L(E) (Energex TM ); diet L(B) (Bio-Feed TM Pro); diet L(a) (Alpha galactosidase TM ); and diet L(Mix), which contained all the enzymes. Fish were randomly stocked into tanks in duplicate groups of 38 ¢sh, 16.58 AE 0.169 (SE) g, and were fed twice a day for 6 weeks. The supplemented enzymes did not improve weight gain in ¢sh fed lupin-based diets. However, mixed enzyme signi¢cantly improved Protein E⁄ciency Ratio (PER). Apparent digestibility of DM, CP and GE signi¢cantly improved in ¢sh-fed L(E) diet. None of the supplemented enzymes a¡ected digestive tract indices or carcass composition. Surprisingly, weight gain was signi¢cantly higher in ¢sh-fed L(a), L(E) and L(Mix) diets as compared with FM diet. Feed intake was signi¢cantly higher in ¢shfed L, L(a) and L(E) diets compared with the FM diet. It is concluded that storing of lupin kernel under a suitable condition may have partially hidden the positive e¡ects of exogenous enzymes through activating the endogenous enzymes.

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“…Improved growth performance and nutrient utilization of Labeo rohita fed DORB based diet supplemented with exogenous enzymes may be contributed to the elimination of anti-nutritional factors and improving the utilization of dietary energy and amino acid. Similar findings were also observed by Farhangi and Carter (2007); Lin et al (2007); Soltan (2009) in rainbow trout, hybrid tilapia and Nile tilapia, respectively, when they fed the diet supplemented with exogenous enzymes.…”

Effects of exogenous dietary enzyme on growth, body composition, and fatty acid profiles of cultured great sturgeon Huso huso fingerlings

“…Final weight of fingerlings fed with the control or enzyme-supplemented diets was between 46.13 and 53.03 g. Beluga fingerlings fed diets 250 mg/kg multienzyme exhibited higher weight gain (53.03 ± 0.15), and better growth rate is obtained by lower dietary enzyme concentration (Table 1). Farhangi and Carter (2007) found that dietary exogenous enzyme supplementation can significantly increase rainbow trout Oncorhynchus mykiss weight gain. In this study, feeding the fish with the diets containing 250 mg/kg resulted in a significantly lower feed conversion ratio compared with that of the control ( Table 1).…”

“…On the other hand, higher growth of fish larvae fed with the live food is attributed to the digestive enzymes activity present in live food (Kolkovski et al 1993). For this reason, in several fish species, e.g., gilthead seabream Sparus auratus (Kolkovski et al 1993), seabass Dicentrarchus labrax (Kolkovski et al 1997), rainbow trout Oncorhynchus mykiss (Farhangi and Carter 2007;Ogunkoya et al 2006), Japanese seabass Lateolabrax japonicus (Ai et al 2007), and African catfish Clarias gariepinus (Yildirim and Turan 2010), the influence of exogenous enzyme on growth performance of larvae has been well investigated.…”

Effects of enzyme complex SSF (solid state fermentation) in pellet diets for Nile tilapia

“…In a study with rainbow trout (Oncorhynchus mykiss), Farhangi & Carter (2007) reported that fish fed diets based on shelled lupines (Lupinus angustifolius) supplemented with α-galactosidase significantly improved weight gain, protein efficiency and apparent digestibility of dry matter and crude protein.…”