Effect of Erica australis extract on Caco-2 cells, fibroblasts and selected pathogenic bacteria responsible for wound infection

Nunes, Ricardo José; Rodrigues, Susana; Paśko, Paweł; Tyszka-Czochara, Małgorzata; Grenha, Ana; Carvalho, Isabel S.

doi:10.1016/j.indcrop.2013.10.015

Cited by 19 publications

(12 citation statements)

References 23 publications

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“…Within the tested concentrations, WOLE presented impact neither on the viability of HTB‐140 and WM793 melanoma cells, nor normal skin fibroblasts. Such results are consistent with our previous study where normal fibroblasts had been reported to have been less susceptible to plant extracts than cancer cell lines . The activity of the tested extract was however significantly lower in comparison to the standard cytotoxic drug used – mitoxantrone ( IC 50 2 – 5 and 0.2 – 0.45 μg/ml for cancer and normal cells, respectively) …”

Section: Resultssupporting

confidence: 92%

Identification of Predominant Phytochemical Compounds and Cytotoxic Activity of Wild Olive Leaves (Olea europaea L. ssp. sylvestris) Harvested in South Portugal

Makowska-Wąs

Galanty

Gdula‐Argasińska

et al. 2017

Chemistry & Biodiversity

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This study has been aimed at providing a qualitative and quantitative evaluation of selected phytochemicals such as phenolic acids, flavonoids, oleuropein, fatty acids profile, and volatile oil compounds, present in wild olive leaves harvested in Portugal, as well as at determining their antioxidant and cytotoxic potential against human melanoma HTB-140 and WM793, prostate cancer DU-145 and PC-3, hepatocellular carcinoma Hep G2 cell lines, as well as normal human skin fibroblasts BJ and prostate epithelial cells PNT2. Gallic, protocatechuic, p-hydroxybenzoic, vanillic acids, apigenin 7-O-glucoside, luteolin 7-O-glucoside, and rutin were identified in olive leaves. The amount of oleuropein was equal to 22.64 g/kg dry weight. (E)-Anethole (32.35%), fenchone (11.89%), and (Z)-3-nonen-1-ol (8%) were found to be the main constituents of the oil volatile fraction, whereas palmitic, oleic, and alpha-linolenic acid were determined to be dominating fatty acids. Olive leaves methanol extract was observed to exerted a significant, selective cytotoxic effect on DU-145 and PC-3 cell lines. Except the essential oil composition, evaluated wild olive leaves, with regard to their quantitative and qualitative composition, do not substantially differ from the leaves of other cultivars grown for industrial purposes and they reveal considerable antioxidant and cytotoxic properties. Thus, the wild species may prove to be suitable for use in traditional medicine as cancer chemoprevention.

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Section: Resultssupporting

confidence: 92%

Identification of Predominant Phytochemical Compounds and Cytotoxic Activity of Wild Olive Leaves (Olea europaea L. ssp. sylvestris) Harvested in South Portugal

Makowska-Wąs

Galanty

Gdula‐Argasińska

et al. 2017

Chemistry & Biodiversity

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“…The optical density was measured at 570 nm (the reference wavelength was 630 nm) using a microplate reader (Tecan Austria GmbH, Grodig, Austria). Results were reported using the following formula: (average OD value of three measurements for each experimental group/average OD value of control group) Â 100% (Nunes et al 2014). …”

Section: Mtt Assaymentioning

confidence: 99%

Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs

Nunes

Paśko

Tyszka-Czochara

et al. 2016

Pharmaceutical Biology

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Context: Crataegus monogyna L. (Rosaceae) (CM), Equisetum telmateia L. (Equisataceae) (ET), Geranium purpureum Vil. (Geraniaceae) (GP), Mentha suaveolens Ehrh. (Lamiaceae) (MS), and Lavandula stoechas L. spp. luisieri (Lamiaceae) (LS) are all medicinal. Objective: To evaluate the antioxidant, antiproliferative and antimicrobial activities of plant extracts and quantify individual phenolics and zinc. Material and methods: Aerial part extracts were prepared with water (W), ethanol (E) and an 80% mixture (80EW). Antioxidant activity was measured with TAA, FRAP and RP methods. Phenolics were quantified with a HPLC. Zinc was quantified using voltammetry. Antibacterial activity (after 48 h) was tested using Enterococcus faecalis, Bacillus cereus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Listeria monocytogenes. Antiproliferative activity (after 24 h) was tested using HEP G2 cells and fibroblasts. Results: Solvents influenced results; the best were E and 80EW. GP had the highest antioxidant activity (TAA and FRAP of 536.90 mg AAE/g dw and 783.48 mg TE/g dw, respectively). CM had the highest zinc concentration (37.21 mg/kg) and phenolic variety, with neochlorogenic acid as the most abundant (92.91 mg/100 g dw). LS was rich in rosmarinic acid (301.71 mg/100 g dw). GP and LS inhibited the most microorganisms: B. cereus, E. coli and S. aureus. GP also inhibited E. faecalis. CM had the lowest MIC: 5830 lg/mL. The antibacterial activity is explained by the phenolics present. LS and CM showed the most significant anti-proliferative activity, which is explained by their zinc content. Conclusion: The most promising plants for further studies are CM, LS and GP.ARTICLE HISTORY

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“…Since ancient times, Erica species have been used as a traditional medicine for the treatment of burns and wounds by the local people in Anatolia. The studies have been showed that Erica species possess antiulcer [8,9], antimicrobial [10,11], antibacterial, cytotoxic [12][13][14][15] and antioxidant [16][17][18][19][20] activities. It has also been reported that Erica species have (poly) phenolic, flavonoid, coumarin and triterpenoid, compounds [21][22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Chemical analysis and in vitro antioxidant and anticholinesterase activities of essential oils and extracts from different parts of Erica manipuliflora

Kuş¹,

Taş²,

Küçükaydın³

et al. 2019

Sanat

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This study reports the chemical composition of essential oils and in vitro antioxidant and anticholinesterase activities of essential oils, hexane, ethyl acetate, methanol, and butanol extracts of aerial, flower and leaves parts of Erica manipuliflora. GC and GC/MS analyses were used for identification of essential oils. Totals of 47 compounds were detected in the essential oils of aerial, flower and leaves parts accounting for 99.99%, 99.88% and 99.97%, respectively. The major components of the aerial, flower and leaves parts of essential oils were Germacren D (14.76%, 15.55% and 13.58%), tau-cadinol (7.53%, 4.11% and 8.96%), caryophyllene oxide (3.92%, 5.17% and 8.55%), βcaryophyllene (7.24%, 5.97% and 7.73%), and α-terpineol (6.85%, 6.14% and 4.18%), respectively. The essential oils of aerial (34.49%) and leaves (37.01%) parts consisted of mainly sesquiterpene hydrocarbons whereas essential oil of flower part (42.58%) included monoterpenoids. The essential oils and extracts were screened for their antioxidant and anticholinesterase activities. The results of activities showed that extracts possessed the highest antioxidant activity while essential oils had the highest anticholinesterase activity. This finding supposes that E. manipuliflora may be considered as valuable natural source with antioxidant and anticholinesterase properties for food, cosmetic and pharmaceutical industries.

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Effect of Erica australis extract on Caco-2 cells, fibroblasts and selected pathogenic bacteria responsible for wound infection

Abstract: 25Plants from the genus Erica are used in many countries to treat several ailments. In this

Cited by 19 publications

References 23 publications

Identification of Predominant Phytochemical Compounds and Cytotoxic Activity of Wild Olive Leaves (Olea europaea L. ssp. sylvestris) Harvested in South Portugal

Identification of Predominant Phytochemical Compounds and Cytotoxic Activity of Wild Olive Leaves (Olea europaea L. ssp. sylvestris) Harvested in South Portugal

Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs

Chemical analysis and in vitro antioxidant and anticholinesterase activities of essential oils and extracts from different parts of Erica manipuliflora

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