Effect of experimental varicocele in rats on testicular oxidative stress status

Koksal, Turker; Erdoğru, Ti̇bet; Toptas, Behiye; Gülkesen, Kemal Hakan; Usta, Murat; Baykal, Aslı; Baykara, Mehmet

doi:10.1046/j.1439-0272.2002.00500.x

Cited by 25 publications

(19 citation statements)

References 34 publications

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“…It was postulated that increasing levels of MDA in testicular biopsies of infertile men are associated with higher grades of varicocele [22]. In another study by the same author, they could not find a relationship between oxidative status and testicular dysfunction in adolescent rats with varicocele [23].…”

Section: Discussionmentioning

confidence: 95%

Testicular Oxidative Stress

Ozdamar

Soylu²,

Çulha³

et al. 2004

Urol Int

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Objective: This present study was undertaken to determine the levels of malondialdehyde (MDA), nitric oxide (NO) and total antioxidant status (TAS) in testes of adolescent rats with experimental bilateral varicocele and to determine the effects of oxidative stress on testis produced by varicocele. Methods: 6-week-old, male Wistar rats, weighing 146–334 g (228.37 ± 41.34 g), were randomly allocated into two groups. The first group underwent selective and bilateral partial ligation of the spermatic vein (n = 28), and the second group underwent a sham operation and served as the controls (n = 15). Animals were sacrificed 12 weeks after surgery and dilatation of the spermatic veins was observed in the first study group. Bilateral orchiectomy was performed in all rats, and MDA, NO and TAS levels were measured. Results: In the study group, the mean MDA (SEM) level was 15.58 ± 6.07 µmol/g protein, and in the control group, it was 11.59 ± 3.86 µmol/g protein, respectively; this difference was statistically significant (p < 0.05). The mean NO level was 82.73 ± 77.84 nmol/g protein in the study group, whereas 28.65 ± 20.18 nmol/g protein in the control group, this difference was also statistically significant (p < 0.005). The mean TAS levels of the study and control groups were 0.91 ± 0.32 and 1.78 ± 0.46 nmol/g tissue, respectively, and this difference was also statistically significant (p < 0.001). But there was no correlation between these three parameters (MDA↔TAS: r = –0.103, p > 0.05; MDA↔NO: r = –0.104, p > 0.05; NO↔TAS: r = –0.123, p > 0.05). Conclusion: These findings suggest that varicocele may change the testicular oxidative status and may play a role in testicular dysfunction that causes infertility.

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Section: Discussionmentioning

confidence: 95%

Testicular Oxidative Stress

Ozdamar

Soylu²,

Çulha³

et al. 2004

Urol Int

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“…However, the exact aetiology of this condition remains unknown. Several studies have suggested that oxidative stress, which is mainly caused by reactive oxygen species (ROS), is involved in the pathogenesis of varicocele in human and rat (Köksal et al, 2000(Köksal et al, , 2002Romeo et al, 2003). Under normal conditions, antioxidants maintain ROS at low levels.…”

Section: Introductionmentioning

confidence: 99%

“…The levels of lipid peroxidation products are increased in testis and semen in patients with varicocele (Sharma and Agarwal, 1996;Köksal et al, 2002). A specific and stable end-product of lipid peroxidation, the aldehyde 4-hydroxy-2-nonenal (4-HNE), can diffuse within, or even escape from, the cell and attack targets far from the site of the original free radical event (Esterbauer et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Increased expression of Leydig cell haem oxygenase-1 preserves spermatogenesis in varicocele

Shiraishi

Naito

2005

Human Reproduction

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BACKGROUND: Oxidative stress is involved in the pathogenesis of testicular disorders. Haem oxygenase-1 (HO-1) plays an important cytoprotective role against oxidative stress. We investigated the presence of oxidative stress, represented by generation of 4-hydroxy-2-nonenal (4-HNE)-modified proteins, and expression of HO-1 in varicocele testes of human. METHODS: Thirty testicular biopsies from patients with left varicocele and 10 from patients with normal spermatogenesis were included. Generation of 4-HNE-modified proteins was examined as a marker of oxidative stress. Expression of HO-1 was assessed by western blotting and immunohistochemistry. The expression was compared with clinico-pathological parameters. RESULTS: Increased generation of 4-HNEmodified proteins was observed in varicocele testes. HO-1 expression was significantly correlated with varicocele grade (P < 0.01 in grade 2 and 3 compared to control) and expression of 4-HNE-modified proteins (r 5 0.508, P < 0.01). The immunoreactivity was increased in Leydig cells in varicocele testes. There were significant correlations between age, total motile sperm count, Johnsen's mean score and HO-1:4-HNE-modified protein ratio (r 5 0.206, 0.405 and 0.408, P 5 0.027, 0.027 and 0.025 respectively). CONCLUSIONS: In testes with varicocele, there are increases in 4-HNE-modified proteins, suggesting that oxidative stress is present. Increased HO-1 expression, mainly in Leydig cells, is considered to protect the cells against oxidative stresses in varicocele testes.

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“…As observed in this study, the MDA level significantly elevated in varicocele-induced rat compared to Control group. MDA levels increased in the semen of patients with varicocele (Koksal et al, 2003;Cervellione et al, 2006) so, increased MDA levels is associated with higher grades of varicocele (Koksal et al, 2002).…”

Section: Discussionmentioning

confidence: 98%

“…Surgical varicocele involves partial obstruction of the left renal vein, causing a varicosity of the left spermatic vein (Koksal et al, 2002). However, the mechanisms responsible for impaired spermatogenesis in animals with iatrogenically induced varicoceles remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Magnesium sulfate protects testis against unilateral varicocele in Rat

2017

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The aim of this study was to investigate the effect of magnesium sulfate (MgSO 4 ) on experimental unilateral varicocele-induced in rats. Sixty male Wistar rats were randomly divided into six experimental groups (n = 10). The Control group had no received any medications and surgery. The Sham group had no received any medication, abdominal cavity was opened without varicocele-induced. Varicocele group: abdominal cavity was opened, varicocele done without any medication treatment. In group 4 abdominal cavity was opened, varicocele-induced then animals orally treated with MgSO 4 (25 mg/kg) for 42 days. The groups 5 and 6 were similar to group 4, except animals received 50 and 100 mg/kg of MgSO 4 , respectively. At the end of days 21 and 42, the abdomen was opened, the left testis extracted for histopathological studies. Also, from the cauda epididymis semen samples were collected to determine malondialdehyde, superoxide dismutase and glutathione peroxidase values. According to the results, there was a significant difference in testis damage grade in Varicocele group compared to the Control group (P < 0.05). The MgSO 4 significantly improved testis damage grade on day 42 (P < 0.05). The MgSO 4 treatment, dose dependently improved seminiferous tubules with many spermatocytes in the seminiferous tubules in experimental unilateral varicocele established rats after 21 and 42 days (P < 0.05). Also, administration of the MgSO 4 via a dose dependent manner significantly normalized malondialdehyde, superoxide dismutase and glutathione peroxidase values to the physiologic level in varicocele-induced rats after 21 and 42 days (P < 0.05). These results suggest administration of the MgSO 4 protects testis against unilateral varicocele in rat.

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Effect of experimental varicocele in rats on testicular oxidative stress status

Cited by 25 publications

References 34 publications

Testicular Oxidative Stress

Testicular Oxidative Stress

Increased expression of Leydig cell haem oxygenase-1 preserves spermatogenesis in varicocele

Magnesium sulfate protects testis against unilateral varicocele in Rat

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