Effect of ferulic acid from Hibiscus mutabilis on filarial parasite Setaria cervi: Molecular and biochemical approaches

Saini, Prasanta; Gayen, Prajna; Nayak, Ananya; Kumar, Deepak; Mukherjee, Niladri; Pal, Buddhadev; Babu, Santi P. Sinha

doi:10.1016/j.parint.2012.04.002

Cited by 50 publications

(30 citation statements)

References 48 publications

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“…Effect of Asparagus adscendens Roxb [19], Effect of alcoholic and aqueous extracts of the fruits of the Ficus racemosa Linn., significantly inhibited the spontaneous movements of the whole worm. [20], ethyl acetate extract of Vitex negundo leaves [21], and in another study Methanolic [22]. Ciprofloxacin was also assessed for antifilarial activity in present research.…”

Section: Disscusionmentioning

confidence: 94%

Antifilarial activity of Butea monosperma L. leaves extracts against Setaria cervi

Deshmukh¹,

Sahare²,

Patidar³

et al. 2014

Trends Vector Res Parasitol

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Background: Usage of herbal drugs in traditional medicine is quite well known but largely empirical. Hence the present study was designed to screen the in vitro antifilarial effect of Butea monosperma L. plant leaves against Setaria cervi. Methods: Antifilarial activity of Methanol/Hexane-ethanol extracts of Butea monosperma L. (leaves) and Ciprofloxacin was explored against adult of Setaria cervi after incubation for 24 hrs with concentration range of 0.25 to 20 mg/ml for possible antifilarial effect by comparing with suitable control, in terms of motility inhibition assay and MTT reduction assay. Results: Butea monosperma L. plant leaves showed significant antifillarial activity against adult as compared to controls whereas activity demonstrated by Ciprofloxacin was comparatively less significant. Inhibitory concentrations (IC50) for the plant extracts with significant antifilarial activity against Setaria cervi adult in vitro system have been derived to be 1.25, 3.6 and 7.5 mg/ml Methanol, Hexane-ethanol extracts and Ciprofloxacin respectively. Conclusion: The present research investigation proved to be an additional frame in recording the plant extract's antifilarial activity. Methanol and Hexane-ethanol extracts of Butea monosperma L. plant leaves which shown significant antifilarial activity. The present research data highlights the importance of further depth research in this area.

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Section: Disscusionmentioning

confidence: 94%

Antifilarial activity of Butea monosperma L. leaves extracts against Setaria cervi

Deshmukh¹,

Sahare²,

Patidar³

et al. 2014

Trends Vector Res Parasitol

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“…Paraffin-embedded sections of control and treated (125 mg/ml of NBE) adult S. cervi were stained with Hoechst 33258 following the method of Saini et al (2012) and micrographed under a UV fluorescence microscope (Olympus, Tokyo, Japan) (Allen et al, 2001). …”

Section: Morphological Analysis Of Apoptosis By Staining With Hoechstmentioning

confidence: 99%

“…cDNA was prepared and the genes were amplified following the method of Saini et al (2012). Expression of apoptotic genes (egl 1, ced 3, and ced 4) and antiapoptotic gene ced 9 was studied.…”

Section: Reverse Transcriptase Polymerase Chain Reactionmentioning

confidence: 99%

“…The level of GSH was measured in treated and control parasites according to the method of Saini et al (2012) using 5,5 0 -dithiobis-(2-nitrobenzoate) [DTNB]. The clear supernatant from adult worm homogenate was mixed with an equal volume of 5% perchloric acid and centrifuged at 805g for 10 min at 4 C. The supernatant (100 ml) thus obtained was mixed with 1.88 ml 0.1 mol/L potassium phosphate buffer, pH 8.0 and 0.02 ml of 4% DTNB, incubated at room temperature for 3 min, and the absorbance was recorded at 412 nm.…”

Section: Enzyme Assaysmentioning

confidence: 99%

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Diospyros perigrenabark extract induced apoptosis in filarial parasiteSetaria cervithrough generation of reactive oxygen species

Saini

Mukherjee

et al. 2015

Pharmaceutical Biology

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Context: Lymphatic filariasis is a major neglected tropical disease. Diospyros perigrena Gurke (Ebenaceae) was selected for antifilarial chemotherapy because of unavailability of proper medicine. In India, different parts of this plant were used for the treatment of diabetes, diarrhea, dysentery, cholera, mouth ulcers, and wounds.Objective: The present study was undertaken to access antifilarial potential and mechanism of action of n-butanol extract (NBE) of D. perigrena stem bark on Setaria cervi Rudolphi (Onchocercidae). Materials and methods: In vitro efficacy and apoptotic mechanism were evaluated by Hoechst, TUNEL, DNA fragmentation assay, pro-and anti-apoptotic gene expression in NBE (250, 125, 62.5, 31.25, and 15.6 mg/ml)-treated S. cervi after 24 h of incubation. Reactive oxygen species (ROS) up-regulation was also determined by GSH, GST, SOD assays, and super oxide anion level.Results: Significant in vitro antifilarial activity of NBE was found 50% inhibitory concentration (IC 50 ): adult ¼ 57.6 mg/ml, microfilariae (mf) ¼ 56.1 mg/ml, and lethal dose (LD 100 ) in mf is 187.17 mg/ml) after 24 h of treatment. NBF-induced apoptosis was proved by Hoechst, TUNEL, RT-PCR, and Western blot method. NBF (250 mg/ml) decreased the level of GSH (17.8%) and GST (65.4%), increased SOD activity (1.42-fold) and super oxide anion production (1.32-fold) in the treated parasite which culminated into ROS up-regulation. Discussion and conclusion: NBE induced apoptosis in different life cycle stages of S. cervi. In future, a detailed study of NBF will give us a novel antifilarial compound which will be used for antifilarial chemotherapy.

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“…FA, a component of Hibiscus mutabilis, has also been demonstrated to have antimicrobial properties against common foodborne bacteria including E. coli, S. aureus, and L. monocytogenes (Borges et al 2013). Ferulic acid (FA) has been also shown to possess antifilarial activity against Setaria cervi (Saini et al 2012). However, the antiviral effects of Hibiscus calyx aqueous extracts and its components against foodborne viruses have not been reported to-date.…”

Section: Introductionmentioning

confidence: 99%

Aqueous Extracts of Hibiscus sabdariffa Calyces Decrease Hepatitis A Virus and Human Norovirus Surrogate Titers

2015

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Hibiscus sabdariffa extract is known to have antioxidant, anti-diabetic, and antimicrobial properties. However, their effects against foodborne viruses are currently unknown. The objective of this study was to determine the antiviral effects of aqueous extracts of H. sabdariffa against human norovirus surrogates (feline calicivirus (FCV-F9) and murine norovirus (MNV-1)) and hepatitis A virus (HAV) at 37 °C over 24 h. Individual viruses (~5 log PFU/ml) were incubated with 40 or 100 mg/ml of aqueous hibiscus extract (HE; pH 3.6), protocatechuic acid (PCA; 3 or 6 mg/ml, pH 3.6), ferulic acid (FA; 0.5 or 1 mg/ml; pH 4.0), malic acid (10 mM; pH 3.0), or phosphate buffered saline (pH 7.2 as control) at 37 °C over 24 h. Each treatment was replicated thrice and plaque assayed in duplicate. FCV-F9 titers were reduced to undetectable levels after 15 min with both 40 and 100 mg/ml HE. MNV-1 was reduced by 1.77 ± 0.10 and 1.88 ± 0.12 log PFU/ml after 6 h with 40 and 100 mg/ml HE, respectively, and to undetectable levels after 24 h by both concentrations. HAV was reduced to undetectable levels by both HE concentrations after 24 h. PCA at 3 mg/ml reduced FCV-F9 titers to undetectable levels after 6 h, MNV-1 by 0.53 ± 0.01 log PFU/ml after 6 h, and caused no significant change in HAV titers. FA reduced FCV-F9 to undetectable levels after 3 h and MNV-1 and HAV after 24 h. Transmission electron microscopy showed no conclusive results. The findings suggest that H. sabdariffa extracts have potential to prevent foodborne viral transmission.

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Effect of ferulic acid from Hibiscus mutabilis on filarial parasite Setaria cervi: Molecular and biochemical approaches

Cited by 50 publications

References 48 publications

Antifilarial activity of Butea monosperma L. leaves extracts against Setaria cervi

Antifilarial activity of Butea monosperma L. leaves extracts against Setaria cervi

Diospyros perigrenabark extract induced apoptosis in filarial parasiteSetaria cervithrough generation of reactive oxygen species

Aqueous Extracts of Hibiscus sabdariffa Calyces Decrease Hepatitis A Virus and Human Norovirus Surrogate Titers

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