Effect of flow on polymorphonuclear leukocyte/endothelial cell adhesion

Abstract

The effect of flow on the adhesion of polymorphonuclear leukocytes (PMNL) to vascular endothelium was investigated using a parallel plate chamber with a well-defined flow field. Washed PMNL were perfused over a monolayer of primary human umbilical vein endothelial cells (HUVEC) pretreated with formyl-methionyl-leucyl-phenylalanine (FMLP, 1 X 10(-7) mol/L) for five minutes. In other experiments HUVEC were pretreated with interleukin 1 (IL1,2 U/mL) for four hours. PMNL adhesion to stimulated and control HUVEC was measured over a physiologic range of wall shear stresses. PMNL adhesion to nylon-coated surface was also studied. At a wall shear stress of 0.98 dynes/cm2,283 +/- 37.3 PMNL/mm2 (mean +/- SEM) adhered to FMLP-treated HUVEC while 195 +/- 20.3 PMNL/mm2 adhered to control HUVEC. At 1.96 dynes/cm2, 68 +/- 14.1 PMNL/mm2 adhered to FMLP-treated HUVEC and 42 +/- 6.0 PMNL/mm2 adhered to control HUVEC. At 3.92 dynes/cm2, virtually no PMNL adherence was noted on either control or FMLP-treated HUVEC. On IL 1-treated HUVEC at 1.96 dynes/cm2, 371 +/- 25.8 PMNL/mm2 adhered while 28 +/- 2.9 PMNL/mm2 adhered to control HUVEC. PMNL adhesion to IL 1-treated and control HUVEC dropped to 10.2 +/- 3.8 and 6.8 +/- 3.5 PMNL/mm2, respectively, at 3.01 dynes/cm2. The effect of flow on PMNL adhesion appears to be an important factor in determining the outcome of the PMNL/HUVEC adhesive interaction under these experimental conditions.

Human melanoma integrins contribute to arrest and stabilization potential while flowing over extracellular matrix

“…A parallel plate flow chamber was constructed from Lexan to form a uniform channel with a small gap-to-width ratio, which ensured an approximation of two infinite parallel plates (for a description see ref. 12). A circular flow chamber design was used in which a polycarbonate shear deck supports a 35 mm tissue culture dish (Coming.…”

Regulation of Leukocyte Recruitment by Local Wall Shear Rate and Leukocyte Delivery

“…also influences leukocyte recruitment to the venular wall. Studies in flow chambers have identified a critical shear threshold for rolling (8,21), have shown that rolling velocity is flow-dependent (1,26), and have shown that firm adhesion is not supported in the presence of elevated shear forces (22). However, the specific role of shear forces on leukocyte recruitment in vivo remains unclear, as indicated by apparent discrepancies between reports from isolated cell systems or in vivo models.…”

Intercellular adhesion molecule-1 and CD18 are involved in neutrophil adhesion and its cytotoxicity to cultured sinusoidal endothelial cells in rats

“…The role of the adhesion molecules in the interaction between the leukocytes and endothelial cells was pathophysiologically investigated in vivo by using an intravital microscope in the mesentric circulation of rats 24 and in vitro by using human umbilical vein endothelial cells in an in vitro adhesion assay. 13,14 These experiments showed that ICAM-1 is important for the adhesion of PMNs to the endothelium. CD11b/CD18 monoclonal antibody is reported to prevent the hepatic infiltration of PMNs and the necrosis of hepatocytes induced by the intraperitoneal injection of endotoxin.…”

“…The adhesion and migration of PMNs on SECs were assessed using an in vitro flow system. 13,14 The chamber con-Å Migrated PMNs 1 100 Ϭ (Adhered PMNs / Migrated PMNs). sisted of a collagen-coated coverslip with a confluent monolayer of SECs that was attached to a polycarbonate base.…”