Effect of Fluocinolone Acetonide on Human Dental Pulp Cells: Cytotoxicity, Proliferation, and Extracellular Matrix Formation

Incorporation of anti-inflammatory agent into calcium hydroxide pulp capping material: An in vitro study of physical and mechanical properties

“…Thus, application of fluocinolone acetonide in vital pulp therapy was discouraged, and it should be used with cautions 27) . Our recent study showed the appropriate concentrations of fluocinolone acetonide in promotion of early healing response of pulp cells were 0.1-10 µmol/ L 18) . Fluocinolone acetonide, therefore, was incorporated into the calcium hydroxide hard setting cement at the selected concentration (50 mmol/L) to release the suitable concentration.…”

“…This period corresponds to the early phase of wound healing, the inflammatory and proliferative phases which start immediately and a few days after the treatment, respectively [28][29][30] . Since 0.1 to 10 µmol/L fluocinolone acetonide has stimulatory effect on cell proliferation and extracellular matrix formation 18) , the initial burst release of fluocinolone acetonide should be beneficial to healing. However, the activity of released fluocinolone acetonide is still not known and has to be confirmed by further in vitro and in vivo studies.…”

“…This might be because uncontrolled release of the steroids which cause unpredictable long-term success and side effects. Recently, a number of evidence has shown the benefits of steroid usage for reduction inflammation and stimulation of pulp healing 7,8,12,18) . Thus, short-term application of a corticosteroid to inflamed dental pulp can be considered to resolve inflammation and consequently promote healing 18) .…”

“…Recently, a number of evidence has shown the benefits of steroid usage for reduction inflammation and stimulation of pulp healing 7,8,12,18) . Thus, short-term application of a corticosteroid to inflamed dental pulp can be considered to resolve inflammation and consequently promote healing 18) . Fluocinolone acetonide is a synthetic corticosteroid commonly used for topical application in the management of dermatologic disorders and oral vesiculoerosive lesions [19][20][21] .…”

“…Increased proliferation of cultured human skin fibroblasts was noted over a wide range of concentrations 23) . Recently, a wide range of concentration of fluocinolone acetonide (0.1-10 µmol/L) demonstrated positive effects on human dental pulp cell proliferation, and both fibronectin and type I collagen synthesis 18) . With the known anti-inflammatory effect, it may have some potential in stimulation of healing of dental pulp tissue before dentin bridge formation.…”