Effect of FSH treatment on cumulus oocyte complex recovery by ovum pick up and in vitro embryo production in beef donor cows

Ongaratto, Felipe Ledur; Cedeño, A.; Rodríguez-Villamil, P.; Tríbulo, A.; Bó, G. A.

doi:10.1016/j.anireprosci.2020.106274

Cited by 17 publications

(22 citation statements)

References 25 publications

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“…The mean number of medium and large follicles significantly (p< 0.05) higher in FSH stimulated group (7.18±0.64 and 5.94±1.0) compared to non-stimulated group (2.19±0.34 and 1.25±0.32) (Table 2). Present results are in accordance with the studies on beef cows (Goodhand et al2000;Jeyakumar, 2004), HF cows (Vieira et al 2014;Silva et al 2017;Ongaratto et al 2020) and in Ongole cattle (Sriman narayana, 2019). It is known that FSH causes the stimulation of follicular growth that caused the increased number of medium and large follicles available for aspiration in FSH stimulated group.…”

Section: Effect Of Fsh Stimulation Of Ovaries On In Vitro Maturation ...supporting

confidence: 92%

“…But the recovery rate was lower in FSH stimulated group (32.24%) compare to non-stimulated group (47.27%) (Table 3). Similarly in HF cows the oocyte recovery rate was low in FSH treated group (60%) than in without FSH stimulation (75%) group (Ongaratto et al 2020). The number oocytes recovered in FSH treated group in the present study were comparable to the earlier studies on HF cows (Jeyakumar, 2004;Aller et al 2010;Vieira et al 2016;Silva et al 2017;Srimannarayana, 2019;Ongaratto et al 2020).…”

Section: Effect Of Fsh Stimulation Of Ovaries On In Vitro Maturation ...supporting

confidence: 86%

“…Similarly in HF cows the oocyte recovery rate was low in FSH treated group (60%) than in without FSH stimulation (75%) group (Ongaratto et al 2020). The number oocytes recovered in FSH treated group in the present study were comparable to the earlier studies on HF cows (Jeyakumar, 2004;Aller et al 2010;Vieira et al 2016;Silva et al 2017;Srimannarayana, 2019;Ongaratto et al 2020). The number oocytes recovered in the present study was lower than the recovery rates of the Ongole cattle (Srimannarayana, 2019).…”

Section: Effect Of Fsh Stimulation Of Ovaries On In Vitro Maturation ...supporting

confidence: 79%

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Effect of FSH Stimulation of Ovaries on in vitro Maturation of Sahiwal Oocytes Collected by Ovum Pick-up (OPU) Method

Vennapureddy

Arunakumari

Reddy

et al. 2022

IJAR

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Background: For conservation of breeds and faster multiplication of superior germplasm in a short period, ovum pick-up- In vitro embryo production (OPU-IVEP) in combination with embryo transfer (ET) can be a viable alternative to the multiple ovulation embryo transfer (MOET). The aim of the present study was to evaluate the effect of FSH stimulation on follicular population, oocyte recovery rate and in vitro maturation of oocytes collected by ovum pick-up in Sahiwal cows. Methods: Sixteen Sahiwal cows aged 3-6 years were randomly divided into two groups. Animals in group 1 (Non-stimulated, n=8) were subjected to ovum pick-up (OPU) twice at 96h interval at random stage of estrous cycle. Animals in group 2 (FSH stimulated, n=8) were subjected to FSH prestimulation (Follitropin-V, 200 mg i/m in 3 tapering doses 100, 60, 40 mg) prior to OPU 1 and with a duration of 96 hrs interval OPU 2 performed. Result: There was significant difference (p less than 0.05) observed between FSH stimulated and non-stimulated group in mean number of follicles available for aspiration (15.31±1.56 and 6.88±0.55, respectively), mean number of oocytes recovered (4.94±0.70 and 3.25±0.40, respectively), cumulus cell expansion rate (92.95 and 83.7% respectively) and 1st polar body extrusion rate (74.64 and 67.44%, respectively). In conclusion, 200 mg of FSH stimulation with decreasing dose prior to OPU increased the follicular population and increased more number of medium and large follicles available for aspiration. FSH pre-treatment also improved the mean number of oocyte recovery and maturation rate compared to non-stimulated group.

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Section: Effect Of Fsh Stimulation Of Ovaries On In Vitro Maturation ...supporting

confidence: 92%

Section: Effect Of Fsh Stimulation Of Ovaries On In Vitro Maturation ...supporting

confidence: 86%

Section: Effect Of Fsh Stimulation Of Ovaries On In Vitro Maturation ...supporting

confidence: 79%

See 1 more Smart Citation

Effect of FSH Stimulation of Ovaries on in vitro Maturation of Sahiwal Oocytes Collected by Ovum Pick-up (OPU) Method

Vennapureddy

Arunakumari

Reddy

et al. 2022

IJAR

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“…FSH has an important role in the cumulus cells expansion and the final maturation of COCs in cows and other mammals (15). The results of the present study showed a significant reduction in FSHr mRNA expression of the matured COCs in SCE group in comparison with that of the NH group.…”

Section: Discussionsupporting

confidence: 50%

“…Growth differentiation factor (GDF9) and steroidogenic acute regulatory (StAR) proteins play a major role in oocyte developmental competence (13,14). In addition, the expression of follicle-stimulating hormone receptor (FSHr) has an important role in the cumulus cells expansion and the final maturation of COCs (15). Oocyte development, follicular growth, and estrous cyclicity were adversely affected in lactating cows with infections of the uterus or mammary gland (16,17).…”

Section: Introductionmentioning

confidence: 99%

Effects of Pre-ovulatory Follicular Fluid of Repeat Breeder Dairy Cows on Bovine Fertility Transcriptomic Markers and Oocytes Maturation and Fertilization Capacity

et al. 2021

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The current study aimed to determine the effects of the preovulatory follicular fluid (FF) of normal heifer (NH) and repeat breeder cows with subclinical endometritis (SCE) or without (nSCE) on oocyte maturation (Experiment 1) and fertilization rates (Experiment 2). Moreover, the pattern of gene expression of cumulus oocyte-complexes was evaluated in Experiment 1. In Experiment 1, nuclear maturation in the nSCE group was higher, compared to that in the SCE group (P = 0.05). In addition, the oocyte nuclear maturation in the normal heifer was significantly higher, in comparison to that of SCE groups (P < 0.05). Furthermore, the mean percentage of normal oocyte fertilization was higher in the nSCE group, compared to that in the SCE group (P < 0.05). The expressions of growth differentiation factor, GDF9; steroidogenic acute regulatory, StAR and follicle-stimulating hormone receptor, FSHr in the NH group were significantly higher, compared to those in SCE and nSCE groups (P < 0.05). Moreover, the expressions of all genes in the nSCE group were not significant, in comparison to those in the SCE group (P > 0.05). The supplementation of oocyte maturation medium with FF from pre-ovulatory follicles of repeat breeder cows resulted in less oocyte maturation and cumulus cell expansion. In conclusion, the lower fertility in RB cows could be ascribed to the lower oocyte maturation rate and less expression of GDF9, StAR, and FSHr in the cumulus-oocyte complexes.

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Effects of hormone sources on developmental competence of oocytes by ovum pickup in Japanese black cattle

Liu,

Chen,

Huang

et al. 2024

Animal Reproduction Science

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Effect of FSH treatment on cumulus oocyte complex recovery by ovum pick up and in vitro embryo production in beef donor cows

Cited by 17 publications

References 25 publications

Effect of FSH Stimulation of Ovaries on in vitro Maturation of Sahiwal Oocytes Collected by Ovum Pick-up (OPU) Method

Effect of FSH Stimulation of Ovaries on in vitro Maturation of Sahiwal Oocytes Collected by Ovum Pick-up (OPU) Method

Effects of Pre-ovulatory Follicular Fluid of Repeat Breeder Dairy Cows on Bovine Fertility Transcriptomic Markers and Oocytes Maturation and Fertilization Capacity

Effects of hormone sources on developmental competence of oocytes by ovum pickup in Japanese black cattle

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