Effect of gamma irradiation on lipid accumulation and expression of regulatory genes involved in lipid biosynthesis in Chlorella sp.

Tale, Manisha P.; Singh, Reema Devi; Kapadnis, B. P.; Ghosh, Subrata

doi:10.1007/s10811-017-1229-9

Cited by 14 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings might be attributed to slight oxidative stress caused by the treatment of gamma irradiation of microalgal species at low doses that led to enhance lipid biosynthesis and therefore increase the lipid accumulation in cells were obtained by irradiated Chlamydomonas sp. and S. ecornis cultures at doses of 25 and 300 Gy, respectively (Tale et al 2018). In a similar investigation, Choi et al (2014) revealed that inducing a mutant strain (Sd−Pm210) was performed by exposing S. dimorphus to a gamma irradiation dose of 800 Gy.…”

Section: Effect Of Gamma Irradiation Doses On the Lipid Content And Productivity Of Microalgaementioning

confidence: 96%

“…Microalgae are considered as the major feedstock of biodiesel due to large lipid amounts that stored into their cells, besides limited requirements for arable soil, as compared to higher plants (e.g. jatropha) (Rawat et al 2011, Medipally et al 2015, Srikanth et al 2015, Tale et al 2018.…”

Section: Introductionmentioning

confidence: 99%

“…lipids, proteins, and carbohydrates) (Abomohra et al 2016). As well, Tale et al (2018) reported that gamma irradiation treatment may also lead to a remarkable rise in the expression of regulatory genes that are basically involved in the biosynthesis of lipids like diacyl˗glycerol acyl˗transferase and acetyl˗CoA carboxylase, and consequently the accumulation of lipids into microalgal cells has increased. Furthermore, lipids contents of other biological materials may positively be affected by gamma irradiation.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of gamma irradiation on the growth and lipid productivity of green microalgae

Moussa

Abo-State

Abdel-Hafez

2020

Arab Universities Journal of Agricultural Sciences

View full text Add to dashboard Cite

In the present research, the impact of exposing Scenedesmus ecornis, S. communis, and Chlamydomonas sp. cultures to doses of gamma irradiation on their growth and productivity of lipids was investigated. Biomass concentration (g/L) of each microalga was periodically determined after exposure to a range of gamma irradiation doses of 0, 25, 50, 75, 100, 200, and 300 Gray (Gy) through 20 days of cultivation. Subsequently, the lipid content (%), and lipid productivity (mg/L day) of each species were evaluated. Results showed that the S. ecornis growth was positively affected by gamma irradiation, that the maximum concentration of biomass was obtained after 15 days at 1.3 g/L by the irradiated S. ecornis exposed to a dose of 300 Gy, while the non˗irradiated culture achieved up to 1.1 g/L. On the other hand, the growths of Chlamydomonas sp. and S. communis were reduced significantly by the radiation treatment. Significant variations have been also observed in the content of lipid and lipid productivity of each microalga. Irradiated S. ecornis at a dose of 300 Gy exhibited the highest content of lipid and lipid productivity to reach 28.4% and 24.9 mg/L day, respectively. Conversely, the best yields of lipid content and lipid productivity were achieved by the non˗irradiated culture of S. communis (24.4% and 16.6 mg/L day, respectively), compared to irradiated culture, regardless of the irradiation dose. The highest lipid content and lipid productivity gained by Chlamydomonas sp. were obtained by the cultures exposed to 25 Gy, being 27.3% and 21.3 mg/L day, respectively. In conclu-sion, results indicated that exposing cells of S. ecornis and Chlamydomonas sp. to specific doses of gamma˗rays has significantly stimulated lipid accumulation into cells, unlike S. communis which was negatively affected by gamma irradiation.

show abstract

Section: Effect Of Gamma Irradiation Doses On the Lipid Content And Productivity Of Microalgaementioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of gamma irradiation on the growth and lipid productivity of green microalgae

Moussa

Abo-State

Abdel-Hafez

2020

Arab Universities Journal of Agricultural Sciences

View full text Add to dashboard Cite

show abstract

“…Although the rational genetic/metabolic engineering of several targets within the genome has significantly enhanced TAPS production, there are still unknown target genes that are difficult to identify with rational approaches. Mutant breeding has been one of the alternative methods for permanently altering a gene(s) to obtain desired features ( Tale et al, 2018 ). This technique usually requires physical mutagens, such as ultraviolet (UV) light and γ-rays ( Lemontt, 1971 ; Lawrence et al, 1985 ; Sitepu et al, 2012 ) to induce mutations within a target genome, and then the mutant strains must be screened to identify those with the desired features.…”

Section: Introductionmentioning

confidence: 99%

Differences in the Fatty Acid Profile, Morphology, and Tetraacetylphytosphingosine-Forming Capability Between Wild-Type and Mutant Wickerhamomyces ciferrii

Choi

Hwang

Cho

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

One tetraacetylphytosphingosine (TAPS)-producing Wickerhamomyces ciferrii mutant was obtained by exposing wild-type W. ciferrii to γ-ray irradiation. The mutant named 736 produced up to 9.1 g/L of TAPS (218.7 mg-TAPS/g-DCW) during batch fermentation in comparison with 1.7 g/L of TAPS (52.2 mg-TAPS/g-DCW) for the wild type. The highest production, 17.7 g/L of TAPS (259.6 mg-TAPS/g-DCW), was obtained during fed-batch fermentation by mutant 736. Fatty acid (FA) analysis revealed an altered cellular FA profile of mutant 736: decrease in C16:0 and C16:1 FA levels, and increase in C18:1 and C18:2 FA levels. Although a significant change in the cellular FA profile was observed, scanning electron micrographs showed that morphology of wild-type and mutant 736 cells was similar. Genetic alteration analysis of eight TAPS biosynthesis-related genes revealed that there are no mutations in these genes in mutant 736; however, mRNA expression analysis indicated 30% higher mRNA expression of TCS10 among the eight genes in mutant 736 than that in the wild-type. Collectively, these results imply that the enhancement of TAPS biosynthesis in mutant 736 may be a consequence of system-level genetic and physiological alterations of a complicated metabolic network. Reverse metabolic engineering based on system-level omics analysis of mutant 736 can make the mutant more suitable for commercial production of TAPS.

show abstract

“…Some of the bioactives associated with skincare are the algal proteins, lipids, vitamins and secondary metabolites such as phenolics, hydrocarbons, pigments etc [12]. (1.33 MeV) has an ability to induce cytological, anatomical, biochemical, morphological and physiological alterations in cells and tissues [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Effect of Gamma Irradiation on Antioxidant Potential and Bioactives of a Cosmeceutically Significant Chlorella Emersonii Kj725233

Sawant

Singh

Ghosh

et al. 2019

Int J Pharm Pharm Sci

Self Cite

View full text Add to dashboard Cite

Objective : Gamma radiation induces free radicals with a corresponding alteration in the cell’s antioxidant defense system. The present study thus aimed at determining the role of gamma irradiation in improving the cosmeceutical potential of CEK in terms of antioxidants. Methods : C.emersonii KJ725233 was subjected to low (100 Gy) and high (1000 Gy) gamma irradiation doses and its effect on the chlorophyll content was evaluated. The quantitative alterations in the antioxidant content of CEK were evaluated by phosphomolybdenum assay (TAC), ferric reducing antioxidant potential (FRAP), 2, 2 - Dipheny-1-picryl hydrazyl radical scavenging assay (DPPH), total phenolic (TPC) and total flavonoid content (TFC). Also, the corresponding qualitative alterations in the bioactives of CEK were determined by GC-HRMS analysis. Results : A 179.57±2.55% increase in the total chlorophyll content alongwith a 71.76±2.96%, 32.08±2.16%, 11.67±0.89%, 42.85±8.0% and 31.37±3.18% increase was observed in the TAC, FRAP, DPPH radical scavenging, TPC and TFC was observed in CEK irradiated at 1000 Gy. GC-HRMS analysis revealed the induction of Vitamin E on irradiation at both the doses with a corresponding decrease in the phytol content whereas 100 Gy stimulated the induction of phytosterols. Conclusion : The potent intrinsic antioxidant activity of cosmeceutically significant CEK can be elevated with the induction of the most sought after antioxidant in cosmetology – Vitamin E on irradiation.

show abstract

Effect of gamma irradiation on lipid accumulation and expression of regulatory genes involved in lipid biosynthesis in Chlorella sp.

Cited by 14 publications

References 30 publications

Effect of gamma irradiation on the growth and lipid productivity of green microalgae

Effect of gamma irradiation on the growth and lipid productivity of green microalgae

Differences in the Fatty Acid Profile, Morphology, and Tetraacetylphytosphingosine-Forming Capability Between Wild-Type and Mutant Wickerhamomyces ciferrii

Effect of Gamma Irradiation on Antioxidant Potential and Bioactives of a Cosmeceutically Significant Chlorella Emersonii Kj725233

Contact Info

Product

Resources

About