Effect of gamma radiation on life history traits of Aedes aegypti (L.)

Shetty, Vinaya; Shetty, N.J.; Harini, B. P.; Ananthanarayana, S. R.; Jha, S. K.; Chaubey, R.C.

doi:10.1016/j.parepi.2016.02.007

Cited by 29 publications

(21 citation statements)

References 26 publications

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“…Indeed, in other studies, Ae . aegypti females that mated with sterile males continued to lay eggs even when males have been exposed to doses in the range 10 to 300 Gy [39, 40]. Similarly, An .…”

Section: Discussionmentioning

confidence: 99%

Optimization of irradiation dose to Aedes aegypti and Ae. albopictus in a sterile insect technique program

et al. 2019

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The sterile insect technique (SIT) may offer a means to control the transmission of mosquito borne diseases. SIT involves the release of male insects that have been sterilized by exposure to ionizing radiation. We determined the effects of different doses of radiation on the survival and reproductive capacity of local strains of Aedes aegypti and Ae . albopictus in southern Mexico. The survival of irradiated pupae was invariably greater than 90% and did not differ significantly in either sex for either species. Irradiation had no significant adverse effects on the flight ability (capacity to fly out of a test device) of male mosquitoes, which consistently exceeded 91% in Ae . aegypti and 96% in Ae . albopictus . The average number of eggs laid per female was significantly reduced in Ae . aegypti at doses of 15 and 30 Gy and no eggs were laid by females that had been exposed to 50 Gy. Similarly, in Ae . albopictus , egg production was reduced at doses of 15 and 25 Gy and was eliminated at 35 Gy. In Ae . aegypti , fertility in males was eliminated at 70 Gy and was eliminated at 30 Gy in females, whereas in Ae . albopictus , the fertility of males that mated with untreated females was almost zero (0.1%) in the 50 Gy treatment and female fertility was eliminated at 35 Gy. Irradiation treatments resulted in reduced ovary length and fewer follicles in both species. The adult median survival time of both species was reduced by irradiation in a dose-dependent manner. However, sterilizing doses of 35 Gy and 50 Gy resulted in little reduction in survival times of males of Ae . albopictus and Ae . aegypti , respectively, indicating that these doses should be suitable for future evaluations of SIT-based control of these species. The results of the present study will be applied to studies of male sexual competitiveness and to stepwise evaluations of the sterile insect technique for population suppression of these vectors in Mexico.

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Section: Discussionmentioning

confidence: 99%

Optimization of irradiation dose to Aedes aegypti and Ae. albopictus in a sterile insect technique program

et al. 2019

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“…However, it may be possible to retain partial fitness if there is also selection for certain life history traits during inbreeding [80]. We show that laboratory populations maintained at low census sizes (N = 100) also experience inbreeding depression, and the loss of fitness correlates strongly with decreased effective population size.…”

Section: Discussionmentioning

confidence: 84%

A comprehensive assessment of inbreeding and laboratory adaptation inAedes aegyptimosquitoes

Ross

Endersby‐Harshman

Hoffmann

2017

Preprint

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Modified Aedes aegypti mosquitoes reared in laboratories are being released around the world to control wild mosquito populations and the diseases they transmit. Several efforts have failed due to poor competitiveness of the released mosquitoes. We hypothesized that colonized mosquito populations could suffer from inbreeding depression and adapt to laboratory conditions, reducing their performance in the field. We established replicate populations of Ae. aegypti mosquitoes collected from Queensland, Australia, and maintained them in the laboratory for twelve generations at different census sizes. Mosquito colonies maintained at small census sizes (≤100 individuals) suffered from inbreeding depression due to low effective population sizes which were only 25% of the census size as estimated by SNP markers. Populations that underwent full-sib mating for 9 consecutive generations had greatly reduced performance across all traits measured. We compared the established laboratory populations with their ancestral population resurrected from quiescent eggs for evidence of laboratory adaptation. The overall performance of laboratory populations maintained at a large census size (400 individuals) increased, potentially reflecting adaptation to artificial rearing conditions. However most individual traits were unaffected, and patterns of adaptation were not consistent across populations. Differences between replicate populations may indicate that founder effects and drift affect experimental outcomes. Though we find limited evidence of laboratory adaptation, mosquitoes maintained at low population sizes can clearly suffer fitness costs, compromising the success of "rear and release" strategies for arbovirus control.

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“…aegypti mosquitoes ranges from 60-80 Gy. Shetty et al (2016) reported the effect of gamma irradiation on doses of 0-50 Gy on fertility, egg hatching, appearance and age of Ae. aegypti mosquitoes.…”

Section: Discussionmentioning

confidence: 99%

A mutant variant of Nix gene on γ-irradiated Aedes aegypti as a male-determination factor

et al. 2019

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Abstract. Ernawan B, Surniyantoro HNE, Sugoro I, Tambunan USF. 2019. A mutant variant of Nix gene on γ-irradiated Aedes aegypti as a male-determination factor. Biodiversitas 20: 893-899. This study was aimed to examine the effect of γ-irradiation on the Nix gene sequence as a male-determination factor on Ae. aegypti. The novelty of the present study is the investigation on the gene mutation level of the deoxyribonucleic acid as an impact of ionizing radiation exposure. Males Ae. aegypti at the pupal stage were sterilized by applying 70 Gy γ-rays in varies dose-rates, i.e., 300, 600, 900, 1200 and 1500 Gy/h utilizing panoramic irradiator and males group without γ-irradiation as controls. Adult males that emerged from the pupal stage were assessed for the Nix gene sequence using a Real-Time Polymerase Chain Reaction method and were sequenced using Clustal Omega and Bioedit software, respectively. The results indicated that mutation occurred in Nix gene sequences were categorized as a point mutation. The change of Nix gene sequences was occurred between the nitrogenous base of guanine to adenine (G>A), thymine to adenine (T>A) and cytosine to adenine (C>A) in certain sequence numbers. There were six and nineteen on the forward-strand and reverse-strand of the Nix gene, respectively, due to γ-irradiation treatment. We conclude that γ-irradiation exposure causes point mutation on the Nix gene of males Ae. aegypti.

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Effect of gamma radiation on life history traits of Aedes aegypti (L.)

Cited by 29 publications

References 26 publications

Optimization of irradiation dose to Aedes aegypti and Ae. albopictus in a sterile insect technique program

Optimization of irradiation dose to Aedes aegypti and Ae. albopictus in a sterile insect technique program

A comprehensive assessment of inbreeding and laboratory adaptation inAedes aegyptimosquitoes

A mutant variant of Nix gene on γ-irradiated Aedes aegypti as a male-determination factor

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