Effect of Gellan Gum/Tuna Skin Film in Guided Bone Regeneration in Artificial Bone Defect in Rabbit Calvaria

Abstract

It is necessary to prevent the invasion of soft tissue into bone defects for successful outcomes in guided bone regeneration (GBR). For this reason, many materials are used as protective barriers to bone defects. In this study, a gellan gum/tuna skin gelatin (GEL/TSG) film was prepared, and its effectiveness in bone regeneration was evaluated. The film exhibited average cell viability in vitro. Experimental bone defects were prepared in rabbit calvaria, and a bone graft procedure with beta-tricalcium phosphate was done. The film was used as a membrane of GBR and compared with results using a commercial collagen membrane. Grafted material did not show dispersion outside of bone defects and the film did not collapse into the bone defect. New bone formation was comparable to that using the collagen membrane. These results suggest that the GEL/TSG film could be used as a membrane for GBR.

Biocompatibility and biodegradation analysis of Nile Tilapia gelatin and apatite membranes

“…Commercial membranes of bovine, porcine or equine xenogeneic collagen have a minimum integrity time of 30 days (ARAÚJO et al, 2021). This reinforces the candidacy of innovative fish biocomposites within the new generation of resorbable membranes for guided bone regeneration (GBR), which can already count on collagenous biopolymers with enzymatic degradation, with rare reports of apatite composites, reabsorbed by multinucleated foreign body giant cells (DANIELETTO-ZANNA et al, 2020;JUNG et al, 2020).…”

“…G elatin has the ability to integrate well into any internal environment, whether animal or human, reducing chronic infl ammation (OGAWA et al, 2017). In the GBR of the calvaria of rabbits using tuna skin gelatin, Jung et al (2020) observed the preservation of the material's mechanical properties and inhibition of soft tissue invasion in the area for up to 4 weeks of the experiment. Sbricoli et al (2020) further reinforce the biomimicry of collagen membranes with the native type I molecule in periodontal connective tissue itself.…”

Recent advances in biofunctional guided bone regeneration materials for repairing defective alveolar and maxillofacial bone: A review

“…This membrane is composed of collagen fibers and glycosaminoglycan, especially hyaluronic acid and chondroitin sulfate, and has a mechanical strength similar to that of commercial GBR collagen membranes, and thus, has the potential to be used for bone repair applications. Jung et al developed a gellan gum/tuna skin gelatin film and evaluated its effectiveness in rabbit calvaria bone regeneration combined with tri-calcium phosphate (TCP) implantation [29] . The repair effect was comparable with that of a commercial collagen membrane.…”

Evaluation of Porcine Collagen Membranes Used with Guided Bone Regeneration for Critical Defects: A Histological, Histomorphometric, Immunohistochemical, and Inflammatory Profile Analysis

“…6 These membranes must have certain fundamental characteristics, such as biocompatibility, stability, and adequate resorption time. 8 Grafts of porcine origin also have a similar degree of compatibility with grafts of human origin. 9 In GBR, success depends on the rate of resorption as well as the membrane's effectiveness as a barrier 8 to remain long enough for repair to occur.…”

“…8 Grafts of porcine origin also have a similar degree of compatibility with grafts of human origin. 9 In GBR, success depends on the rate of resorption as well as the membrane's effectiveness as a barrier 8 to remain long enough for repair to occur. 4 A wide variety of CMs are on the market, such as Bio-Gide (Geistlich Pharma AG, Wolhusen, Switzerland), which has high density and low porosity and is one of the most studied and utilized membranes in regeneration processes.…”