Effect of Guanethidine-Induced Sympathectomy on Osteoblastic Activity in the Rat Femur Evaluated by ³H·Proline Autoradiography

Abstract

Osteoblastic activity in the rat femur was assessed following sympathectomy by injections of guanethidine sulfate from birth to 14 days of age. At ages 30, 45 and 90 days, osteogenesis was monitored by quantitative autoradiography using ³H-proline. Grain counts over periosteal osteoblasts of the femoral diaphysis showed a significant reduction in the uptake of ³H -proline in sympathectomized rats. The results indicate that the sympathetic innervation of bone influences osteoblastic activity and provide support for a role of the autonomic nervous system in the regulation of bone formation.

Treatment with beta-blockers, ACE inhibitors, and calcium-channel blockers is associated with a reduced fracture risk: a nationwide case–control study

“…In-vitro studies in different lines of bone cells, as well as in-vivo studies in experimental animals, have demonstrated effects of b-adrenergic receptor agonists and antagonists on bone metabolism, but the results reported have been conflicting. In different studies, b-adrenergic stimulation and inhibition have been reported to cause Antihypertensive drugs and fracture risk Rejnmark et al 585 anabolic as well as catabolic effects on bone [23][24][25][26][27]. In humans, there are only few and conflicting data on the effects of beta-blockers on bone metabolism and fracture risk.…”

Neuronal Activity in Ontogeny and Oncology

“…The sympathetic nervous system influences both osteoblast and osteoclast activity. Chemically sympathectomized mice demonstrate a decrease in osteoblastic activity, with reduced uptake of collagen precursors and deposition of the bone matrix [145,146]. Both β-adrenergic and glutamatergic signaling influences osteoblast function and bone remodeling.…”

Adrenergic regulation of bone metabolism: Possible involvement of sympathetic innervation of osteoblastic and osteoclastic cells

“…Singh et al (1981) and Herskovits and Singh (1984) demonstrated the modulation of osteoblastic activity following sympathectomy induced by guanethidine, which specifically destroys sympathetic adrenergic fibers.…”