Effect of guanethidine on dopamine in small intensely fluorescent cells of the superior cervical ganglion of the rat

Chemosensitivity, plasticity, and functional heterogeneity of paraganglionic cells in the rat coeliac‐superior mesenteric complex

“…The present findings confirm previous data based upon fluorescence histochemistry (Madariaga-Domich and Taxi, 1987;Santer et al, 1975) or immunohistochemistry (Borghini, 1992;Borghini et al, 1991a) that indicate paraganglionic cells are more abundant in the CSMC than in the superior cervical ganglion. Assuming that the number of neurons is approximately 13,000 (Baker and Santer, 1988), it can be deduced that paraganglionic cells in the CSMC represent 5-10% of the total catecholaminergic cell population.…”

“…Guanethidine has been demonstrated to be selectively accumulated in sympathetic neurons but not within other cell types, such as chromaffin and glomus cells or sensory neurons (Burnstock et al, 1971;Eranko and Eranko, 1971a,b;Jensen-Holm and Juul, 1970). In a previous study we have shown that chemical sympathectomy greatly reduced the number of noradrenergic neurons but increased the number of dopaminergic SIF cells in the rat superior cervical ganglion by 80% (Borghini et al 1991a). …”

Epidermal adrenergic signaling contributes to inflammation and pain sensitization in a rat model of complex regional pain syndrome

“…The guanethidine treatment was started at 14 days post-fracture and continued for 2 weeks (a total of 14 daily treatments, 50 mg/kg, subcutaneous injection, s.c., daily). This treatment protocol severely depletes NE levels in the peripheral sympathetic fibers [4; 37]. There were 2 sets of controls for these experiments, control rats that had no fracture or injections, and fracture control rats that were fractured/casted and received saline injections.…”