Effect of hemolysis on insulin determination by the Beckman Coulter Unicell DXI 800 immunoassay analyzer

Cook, Paul; Glenn, Christine; Armston, Annie

doi:10.1016/j.clinbiochem.2010.01.002

Cited by 21 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the appraisal of samples’ long‐term storage stability up to 12 months at −80 °C and −150 °C, FluidX® storage tubes (FluidX Ltd. Monks Heath Hall, Nether Alderley, United Kingdom) were used. The influence of hemolysis was investigated according to Cook et al …”

Section: Methodsmentioning

confidence: 99%

Targeted On‐line SPE‐LC‐MS/MS Assay for the Quantitation of 12 Apolipoproteins from Human Blood

et al. 2018

View full text Add to dashboard Cite

Laborious sample pretreatment of biological samples represents the most limiting factor for the translation of targeted proteomics assays from research to clinical routine. An optimized method for the simultaneous quantitation of 12 major apolipoproteins (apos) combining on-line SPE and fast LC-MS/MS analysis in 6.5 min total run time was developed, reducing the manual sample pretreatment time of 3 μL serum or plasma by 60%. Within-run and between-day imprecisions below 10 and 15% (n = 10) and high recovery rates (94-131%) were obtained applying the high-throughput setup. High-quality porcine trypsin was used, which outperformed cost-effective bovine trypsin regarding digestion efficiency. Comparisons with immunoassays and another LC-MS/MS assay demonstrated good correlation (Pearson's R: 0.81-0.98). Further, requirements on sample quality concerning sampling, processing, and long-term storage up to 1 year were investigated revealing significant influences of the applied sampling material and coagulant on quantitation results. Apo profiles of 1339 subjects of the LIFE-Adult-Study were associated with lifestyle and physiological parameters as well as establish parameters of lipid metabolism (e.g., triglycerides, cholesterol). Besides gender effects, most significant impact was seen regarding lipid-lowering medication. In conclusion, this novel highly standardized, high-throughput targeted proteomics assay utilizes a fast, simultaneous analysis of 12 apos from least sample amounts.

show abstract

Section: Methodsmentioning

confidence: 99%

Targeted On‐line SPE‐LC‐MS/MS Assay for the Quantitation of 12 Apolipoproteins from Human Blood

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Four ELISA kits for human insulin (10‐1113‐01, Mercodia; 80‐INSHU‐E01.1, Alpco; KT‐886, Epitope Diagnostics; ab200011, Abcam) were purchased from the respective manufacturers. Three CLIA kits were equipped with fully automated analyzers (Siemens Centaur XP, Siemens Healthineers; Unicel Dxl800, Beckman Coulter Diagnostics; Cobas e801, Roche Diagnostics) including reagents and necessary parts 15–17 . All kits are intended to be used to research and in‐vitro diagnosis depending on regional permission.…”

Section: Methodsmentioning

confidence: 99%

“…Three CLIA kits were equipped with fully automated analyzers (Siemens Centaur XP, Siemens Healthineers; Unicel Dxl800, Beckman Coulter Diagnostics; Cobas e801, Roche Diagnostics) including reagents and necessary parts. [15][16][17] All kits are intended to be used to research and in-vitro diagnosis depending on regional permission. Pooled human serum was obtained from Innovative Research (Canada) followed by homogenization, filtration, and aliquots in-house protocol (Supporting Information).…”

Section: Chemicalsmentioning

confidence: 99%

Measurement comparability of insulin assays using conventional immunoassay kits

Rosli

Kwon

Lim

et al. 2022

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Immunoassays are analytical methods that achieve the detection and quantification of analytes, particularly peptides and proteins in biological samples, through the formation of a stable complex between the analyte and a specific antibody. They represent very selective and sensitive techniques that have found application in several areas such as clinical chemistry, bioanalysis, pharmaceutical analysis, toxicological analysis, and environmental analysis. [1][2][3] Owing to their capacity for high throughput and significantly reduced average analytical times, through the simultaneous analysis of numerous samples with ultimate detection sensitivity, immunoassays are the preferred platform for most protein studies, particularly clinical diagnostics and drug development where specificity is critical. 4-6 Among various immunoassays, the most commonly employed in routine clinical settings are the enzyme-linked immunosorbent assay (ELISA)

show abstract

“…It is possible that different therapeutic molecules and their respective methods may show differences or different risks for such interference. It was discussed that examples had been observed where variations in matrices impacted final bioanalytical concentration values, one example being hemolysis effects on some insulin assays [14,15].…”

Section: Testing For Hemolysis and Lipemia Effects During Lba Methods Validationmentioning

confidence: 99%

2015 White Paper on Recent Issues in Bioanalysis: Focus on New Technologies and Biomarkers (Part 3 – Lba, Biomarkers and Immunogenicity)

Amaravadi

Song²,

Myler

et al. 2015

Bioanalysis

View full text Add to dashboard Cite

The 2015 9th Workshop on Recent Issues in Bioanalysis (9th WRIB) took place in Miami, Florida with participation of 600 professionals from pharmaceutical and biopharmaceutical companies, biotechnology companies, contract research organizations and regulatory agencies worldwide. WRIB was once again a 5 day, week-long event - A Full Immersion Bioanalytical Week - specifically designed to facilitate sharing, reviewing, discussing and agreeing on approaches to address the most current issues of interest in bioanalysis. The topics covered included both small and large molecules, and involved LCMS, hybrid LBA/LCMS and LBA approaches, including the focus on biomarkers and immunogenicity. This 2015 White Paper encompasses recommendations emerging from the extensive discussions held during the workshop, and is aimed to provide the bioanalytical community with key information and practical solutions on topics and issues addressed, in an effort to enable advances in scientific excellence, improved quality and better regulatory compliance. Due to its length, the 2015 edition of this comprehensive White Paper has been divided into three parts. Part 3 discusses the recommendations for large molecule bioanalysis using LBA, biomarkers and immunogenicity. Part 1 (small molecule bioanalysis using LCMS) and Part 2 (hybrid LBA/LCMS and regulatory inputs from major global health authorities) have been published in volume 7, issues 22 and 23 of Bioanalysis, respectively.

show abstract

Effect of hemolysis on insulin determination by the Beckman Coulter Unicell DXI 800 immunoassay analyzer

Cited by 21 publications

References 8 publications

Targeted On‐line SPE‐LC‐MS/MS Assay for the Quantitation of 12 Apolipoproteins from Human Blood

Targeted On‐line SPE‐LC‐MS/MS Assay for the Quantitation of 12 Apolipoproteins from Human Blood

Measurement comparability of insulin assays using conventional immunoassay kits

2015 White Paper on Recent Issues in Bioanalysis: Focus on New Technologies and Biomarkers (Part 3 – Lba, Biomarkers and Immunogenicity)

Contact Info

Product

Resources

About