Effect of heterologous expression of genes involved in the elongation cycle of fatty acid synthesis on fatty acid production in Saccharomyces cerevisiae

Jung, Yeontae; Kim, Sangwoo; Lee, Sunhee; Ha, Kyoung-Su; Lee, Jinwon

doi:10.1007/s12257-014-0484-8

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…To reveal the molecular mechanism underlying the ecological adaptation of Sillago species, we estimated the dN/dS to identify the PSGs of Sillago species. A total of 44 orthologous genes were identified to be positively selected and might be involved in many biological processes, including the stress response (LTV1 [44], SMO [45], PSA [46,47], ABCB7 [48], UBIAD1 [49], COPA [50], MED27 [51,52], MED28 [51,52], SF3A1 [53], SF3B5 [53], TFIP11 [54], NUDT6 [55], POLλ [56], and DEPDC5 [57]), energy metabolism (APF [58] and IMMT [59]), carbohydrate metabolism (SUCLG1 [60]), amino acid metabolism (GCN4 [61] and CPD [62]), lipid metabolism (HUWE1 [63] and FABZ [64]), visual sense (AP4B1 [65] and PRPF8 [66]), growth and differentiation (ABTB1 [67], UBE4A [68], DOHH [69], and GAB1 [70]), embryogenesis (SBDS [71], TAF5L [72], and SEC8 [73]), and others.…”

Section: Positively Selected Genes Might Contribute To the Ecologicalmentioning

confidence: 99%

Comprehensive Transcriptome Analysis Reveals Insights into Phylogeny and Positively Selected Genes of Sillago Species

Lou

Zhang

Song

et al. 2020

Animals

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Sillago species lives in the demersal environments and face multiple stressors, such as localized oxygen depletion, sulfide accumulation, and high turbidity. In this study, we performed transcriptome analyses of seven Sillago species to provide insights into the phylogeny and positively selected genes of this species. After de novo assembly, 82,024, 58,102, 63,807, 85,990, 102,185, 69,748, and 102,903 unigenes were generated from S. japonica, S. aeolus, S. sp.1, S. sihama, S. sp.2, S. parvisquamis, and S. sinica, respectively. Furthermore, 140 shared orthologous exon markers were identified and then applied to reconstruct the phylogenetic relationships of the seven Sillago species. The reconstructed phylogenetic structure was significantly congruent with the prevailing morphological and molecular biological view of Sillago species relationships. In addition, a total of 44 genes were identified to be positively selected, and these genes were potential participants in the stress response, material (carbohydrate, amino acid and lipid) and energy metabolism, growth and differentiation, embryogenesis, visual sense, and other biological processes. We suspected that these genes possibly allowed Sillago species to increase their ecological adaptation to multiple environmental stressors.

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Section: Positively Selected Genes Might Contribute To the Ecologicalmentioning

confidence: 99%

Comprehensive Transcriptome Analysis Reveals Insights into Phylogeny and Positively Selected Genes of Sillago Species

Lou

Zhang

Song

et al. 2020

Animals

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“…The expression of type II FAS systems in S. cerevisiae have also been reported in four separate publications ( Table 1 , #2, #4, #5, #6). The separate expression of individual genes belonging to the FASII from Streptococcus pyogenes in yeast resulted in increased production of C16 and C18 fatty acids ( Jung et al, 2015 ) ( Table 1 , #2). Another report describes the expression of the FASII from E. coli successfully providing lipoic acid (C8) for a heterologous pyruvate dehydrogenase ( Lian and Zhao, 2016 ) ( Table 1 , #5).…”

Section: Introductionmentioning

confidence: 99%

Optimization of a hybrid bacterial/Arabidopsis thaliana fatty acid synthase system II in Saccharomyces cerevisiae

Pozdniakova

Cruz

Silva

et al. 2023

Metabolic Engineering Communications

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Heterologous expression of AoD9D enhances salt tolerance with increased accumulation of unsaturated fatty acid in transgenic Saccharomyces cerevisiae

Hú

et al. 2019

Journal of Industrial Microbiology and Biotechnology

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Salt stress can trigger several physiological responses in microorganisms such as the increasing accumulation of unsaturated fatty acid, which was biosynthesized by delta-9 fatty acid desaturases (D9D) at the first step. In the present study, two D9D genes, designated AoD9D1 and AoD9D2, were isolated from Aspergillus oryzae. The expression analysis showed that AoD9D1 and AoD9D2 were upregulated under salt stress. To investigate the function of AoD9D, transgenic Saccharomyces cerevisiae strains that heterologously expressed AoD9D were exposed to salinity condition. These transgenic strains exhibited greater tolerance to salt stress than wild-type strains, and the heterologous expression of AoD9D increased the content in unsaturated fatty acids as compared to control cells. Moreover, AoD9D1 and AoD9D2 both contained fatty acid desaturase (FAD) and cytochrome b5-like Heme/Steroid-binding domains (Cyt-b5). S. cerevisiae separately transformed with the gene fragments coding for the FAD and Cyt-b5 domains in the AoD9D1 protein grew better and accumulated a higher concentration of unsaturated FAs than the control. Altogether, the heterologous expression of AoD9D enhanced the tolerance of transgenic S. cerevisiae to high salinity stress with increased accumulation of unsaturated fatty acid. The results provide some practical basis for the successful development of salt-tolerant fermentation microorganisms.

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Effect of heterologous expression of genes involved in the elongation cycle of fatty acid synthesis on fatty acid production in Saccharomyces cerevisiae

Cited by 3 publications

References 21 publications

Comprehensive Transcriptome Analysis Reveals Insights into Phylogeny and Positively Selected Genes of Sillago Species

Comprehensive Transcriptome Analysis Reveals Insights into Phylogeny and Positively Selected Genes of Sillago Species

Optimization of a hybrid bacterial/Arabidopsis thaliana fatty acid synthase system II in Saccharomyces cerevisiae

Heterologous expression of AoD9D enhances salt tolerance with increased accumulation of unsaturated fatty acid in transgenic Saccharomyces cerevisiae

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